Development of a genomic in situ hybridization method using Technovit 7100 sections of early wheat embryo

K. Mochida, H. Tsujimoto

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

It is difficult to observe the behavior of chromosomes in early wheat embryos because they are wrapped in several cell layers of the ovary. Here we conducted genomic in situ hybridization on sections of ovary embedded in Technovit 7100, a resinous compound suitable for in situ hybridization of mRNA in sectioned tissues. With this resin it is possible to make thin sections with high resolution, no autofluorescence, and good water permeability. These features enable histochemical study using fluorescence microscopy. We established the most suitable conditions for the denaturation of target DNA embedded in Technovit resin, and performed GISH on them. Using this method, we identified Leymus mollis chromosomes in the young ovary of F1 hybrids between wheat and L. mollis. Furthermore, we observed the behavior of maize chromosomes in early wheat x maize hybrid embryos.

Original languageEnglish
Pages (from-to)257-260
Number of pages4
JournalBiotechnic and Histochemistry
Volume76
Issue number5-6
Publication statusPublished - Dec 1 2001
Externally publishedYes

Keywords

  • GISH
  • Glycol methacrylate resin
  • Super-wide cross
  • Technovit 7100
  • Triticum aestivum

ASJC Scopus subject areas

  • Histology
  • Medical Laboratory Technology

Fingerprint Dive into the research topics of 'Development of a genomic in situ hybridization method using Technovit 7100 sections of early wheat embryo'. Together they form a unique fingerprint.

Cite this