Development of a genomic in situ hybridization method using Technovit 7100 sections of early wheat embryo

Keiichi Mochida, H. Tsujimoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

It is difficult to observe the behavior of chromosomes in early wheat embryos because they are wrapped in several cell layers of the ovary. Here we conducted genomic in situ hybridization on sections of ovary embedded in Technovit 7100, a resinous compound suitable for in situ hybridization of mRNA in sectioned tissues. With this resin it is possible to make thin sections with high resolution, no autofluorescence, and good water permeability. These features enable histochemical study using fluorescence microscopy. We established the most suitable conditions for the denaturation of target DNA embedded in Technovit resin, and performed GISH on them. Using this method, we identified Leymus mollis chromosomes in the young ovary of F1 hybrids between wheat and L. mollis. Furthermore, we observed the behavior of maize chromosomes in early wheat x maize hybrid embryos.

Original languageEnglish
Pages (from-to)257-260
Number of pages4
JournalBiotechnic and Histochemistry
Volume76
Issue number5-6
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Triticum
In Situ Hybridization
Ovary
Embryonic Structures
Chromosomes
Zea mays
Nucleic Acid Denaturation
Fluorescence Microscopy
Permeability
Messenger RNA
Water
Technovit 7100

Keywords

  • GISH
  • Glycol methacrylate resin
  • Super-wide cross
  • Technovit 7100
  • Triticum aestivum

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Anatomy

Cite this

Development of a genomic in situ hybridization method using Technovit 7100 sections of early wheat embryo. / Mochida, Keiichi; Tsujimoto, H.

In: Biotechnic and Histochemistry, Vol. 76, No. 5-6, 2001, p. 257-260.

Research output: Contribution to journalArticle

@article{036b5c41f20a42c2a6efc592f777f9e7,
title = "Development of a genomic in situ hybridization method using Technovit 7100 sections of early wheat embryo",
abstract = "It is difficult to observe the behavior of chromosomes in early wheat embryos because they are wrapped in several cell layers of the ovary. Here we conducted genomic in situ hybridization on sections of ovary embedded in Technovit 7100, a resinous compound suitable for in situ hybridization of mRNA in sectioned tissues. With this resin it is possible to make thin sections with high resolution, no autofluorescence, and good water permeability. These features enable histochemical study using fluorescence microscopy. We established the most suitable conditions for the denaturation of target DNA embedded in Technovit resin, and performed GISH on them. Using this method, we identified Leymus mollis chromosomes in the young ovary of F1 hybrids between wheat and L. mollis. Furthermore, we observed the behavior of maize chromosomes in early wheat x maize hybrid embryos.",
keywords = "GISH, Glycol methacrylate resin, Super-wide cross, Technovit 7100, Triticum aestivum",
author = "Keiichi Mochida and H. Tsujimoto",
year = "2001",
language = "English",
volume = "76",
pages = "257--260",
journal = "Biotechnic and Histochemistry",
issn = "1052-0295",
publisher = "Informa Healthcare",
number = "5-6",

}

TY - JOUR

T1 - Development of a genomic in situ hybridization method using Technovit 7100 sections of early wheat embryo

AU - Mochida, Keiichi

AU - Tsujimoto, H.

PY - 2001

Y1 - 2001

N2 - It is difficult to observe the behavior of chromosomes in early wheat embryos because they are wrapped in several cell layers of the ovary. Here we conducted genomic in situ hybridization on sections of ovary embedded in Technovit 7100, a resinous compound suitable for in situ hybridization of mRNA in sectioned tissues. With this resin it is possible to make thin sections with high resolution, no autofluorescence, and good water permeability. These features enable histochemical study using fluorescence microscopy. We established the most suitable conditions for the denaturation of target DNA embedded in Technovit resin, and performed GISH on them. Using this method, we identified Leymus mollis chromosomes in the young ovary of F1 hybrids between wheat and L. mollis. Furthermore, we observed the behavior of maize chromosomes in early wheat x maize hybrid embryos.

AB - It is difficult to observe the behavior of chromosomes in early wheat embryos because they are wrapped in several cell layers of the ovary. Here we conducted genomic in situ hybridization on sections of ovary embedded in Technovit 7100, a resinous compound suitable for in situ hybridization of mRNA in sectioned tissues. With this resin it is possible to make thin sections with high resolution, no autofluorescence, and good water permeability. These features enable histochemical study using fluorescence microscopy. We established the most suitable conditions for the denaturation of target DNA embedded in Technovit resin, and performed GISH on them. Using this method, we identified Leymus mollis chromosomes in the young ovary of F1 hybrids between wheat and L. mollis. Furthermore, we observed the behavior of maize chromosomes in early wheat x maize hybrid embryos.

KW - GISH

KW - Glycol methacrylate resin

KW - Super-wide cross

KW - Technovit 7100

KW - Triticum aestivum

UR - http://www.scopus.com/inward/record.url?scp=0035691886&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035691886&partnerID=8YFLogxK

M3 - Article

C2 - 11871747

AN - SCOPUS:0035691886

VL - 76

SP - 257

EP - 260

JO - Biotechnic and Histochemistry

JF - Biotechnic and Histochemistry

SN - 1052-0295

IS - 5-6

ER -