Development of a dynamic micro RI imaging system for single cells

G. Hirumi, F. Nishikido, H. Tashima, H. Wakizaka, T. Higuchi, H. Haneishi, T. Yamaya

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Studies on cell regulation are attracting worldwide attention in order to realize regenerative medicine. Therefore, a nuclear medicine imaging method, which can use tracers having substantially the same composition as a target biomolecule, is required. Autoradiography is a high-resolution nuclear medicine imaging method. However, this method does not have a dynamical imaging capability due to the principle on which it is based. In this research, we develop a nuclear medicine imaging system for dynamic cell observation. Specifically, in order to prevent broadening of the scintillation position because of scintillation light spreading in the scintillator, β-rays are detected by a thin scintillator plate. A scientific CMOS camera with low readout noise and high resolution was used to detect scintillation light. The scintillator plate was a CsI crystal connected to an optical fiber array. The scintillation light generated from the scintillator plate was extracted through optical fibers. The imaging lenses adopted in this research were a high-speed imaging and a conversion lens.Imaging resolution was adjusted to 6.5 × 6.5 mm 2 / pixel and exposure time was 10 s. In order to evaluate the imaging performance of the proposed system, we measured samples containing a radiopharmaceutical with a prototype system. In this measurement, two different activity 18 F-solutions were used as the imaging target. They were fixed with a uniform thickness of 0.4 mm. In the imaging results, we measured different image values depending on their activities. On the other hand, in the region of no 18 F-solutions, the image value was almost constant. The output had sufficient linearity for images with activity exceeding 0.05 Bq / pixel. Imaging results showed that our system has sufficient sensitivity for imaging uptake of 18 F by single cells.

Original languageEnglish
Title of host publication2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017 - Conference Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781538622827
DOIs
Publication statusPublished - Nov 12 2018
Externally publishedYes
Event2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017 - Atlanta, United States
Duration: Oct 21 2017Oct 28 2017

Publication series

Name2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017 - Conference Proceedings

Conference

Conference2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017
CountryUnited States
CityAtlanta
Period10/21/1710/28/17

Fingerprint

Nuclear Medicine
Imaging systems
scintillation counters
nuclear medicine
scintillation
Optical Fibers
Imaging techniques
Light
Lenses
cells
Regenerative Medicine
Scintillation
Radiopharmaceuticals
optical fibers
pixels
lenses
Nuclear medicine
Phosphors
Autoradiography
autoradiography

ASJC Scopus subject areas

  • Instrumentation
  • Radiology Nuclear Medicine and imaging
  • Nuclear and High Energy Physics

Cite this

Hirumi, G., Nishikido, F., Tashima, H., Wakizaka, H., Higuchi, T., Haneishi, H., & Yamaya, T. (2018). Development of a dynamic micro RI imaging system for single cells. In 2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017 - Conference Proceedings [8533008] (2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017 - Conference Proceedings). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NSSMIC.2017.8533008

Development of a dynamic micro RI imaging system for single cells. / Hirumi, G.; Nishikido, F.; Tashima, H.; Wakizaka, H.; Higuchi, T.; Haneishi, H.; Yamaya, T.

2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017 - Conference Proceedings. Institute of Electrical and Electronics Engineers Inc., 2018. 8533008 (2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017 - Conference Proceedings).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hirumi, G, Nishikido, F, Tashima, H, Wakizaka, H, Higuchi, T, Haneishi, H & Yamaya, T 2018, Development of a dynamic micro RI imaging system for single cells. in 2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017 - Conference Proceedings., 8533008, 2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017 - Conference Proceedings, Institute of Electrical and Electronics Engineers Inc., 2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017, Atlanta, United States, 10/21/17. https://doi.org/10.1109/NSSMIC.2017.8533008
Hirumi G, Nishikido F, Tashima H, Wakizaka H, Higuchi T, Haneishi H et al. Development of a dynamic micro RI imaging system for single cells. In 2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017 - Conference Proceedings. Institute of Electrical and Electronics Engineers Inc. 2018. 8533008. (2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017 - Conference Proceedings). https://doi.org/10.1109/NSSMIC.2017.8533008
Hirumi, G. ; Nishikido, F. ; Tashima, H. ; Wakizaka, H. ; Higuchi, T. ; Haneishi, H. ; Yamaya, T. / Development of a dynamic micro RI imaging system for single cells. 2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017 - Conference Proceedings. Institute of Electrical and Electronics Engineers Inc., 2018. (2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017 - Conference Proceedings).
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