Development of a dynamic micro RI imaging system for single cells

G. Hirumi, F. Nishikido, H. Tashima, H. Wakizaka, T. Higuchi, H. Haneishi, T. Yamaya

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Studies on cell regulation are attracting worldwide attention in order to realize regenerative medicine. Therefore, a nuclear medicine imaging method, which can use tracers having substantially the same composition as a target biomolecule, is required. Autoradiography is a high-resolution nuclear medicine imaging method. However, this method does not have a dynamical imaging capability due to the principle on which it is based. In this research, we develop a nuclear medicine imaging system for dynamic cell observation. Specifically, in order to prevent broadening of the scintillation position because of scintillation light spreading in the scintillator, β-rays are detected by a thin scintillator plate. A scientific CMOS camera with low readout noise and high resolution was used to detect scintillation light. The scintillator plate was a CsI crystal connected to an optical fiber array. The scintillation light generated from the scintillator plate was extracted through optical fibers. The imaging lenses adopted in this research were a high-speed imaging and a conversion lens.Imaging resolution was adjusted to 6.5 × 6.5 mm 2 / pixel and exposure time was 10 s. In order to evaluate the imaging performance of the proposed system, we measured samples containing a radiopharmaceutical with a prototype system. In this measurement, two different activity 18 F-solutions were used as the imaging target. They were fixed with a uniform thickness of 0.4 mm. In the imaging results, we measured different image values depending on their activities. On the other hand, in the region of no 18 F-solutions, the image value was almost constant. The output had sufficient linearity for images with activity exceeding 0.05 Bq / pixel. Imaging results showed that our system has sufficient sensitivity for imaging uptake of 18 F by single cells.

Original languageEnglish
Title of host publication2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017 - Conference Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781538622827
DOIs
Publication statusPublished - Nov 12 2018
Externally publishedYes
Event2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017 - Atlanta, United States
Duration: Oct 21 2017Oct 28 2017

Publication series

Name2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017 - Conference Proceedings

Conference

Conference2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017
CountryUnited States
CityAtlanta
Period10/21/1710/28/17

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ASJC Scopus subject areas

  • Instrumentation
  • Radiology Nuclear Medicine and imaging
  • Nuclear and High Energy Physics

Cite this

Hirumi, G., Nishikido, F., Tashima, H., Wakizaka, H., Higuchi, T., Haneishi, H., & Yamaya, T. (2018). Development of a dynamic micro RI imaging system for single cells. In 2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017 - Conference Proceedings [8533008] (2017 IEEE Nuclear Science Symposium and Medical Imaging Conference, NSS/MIC 2017 - Conference Proceedings). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NSSMIC.2017.8533008