Development of a cylindrical apparatus for membrane-surface liquid culture and production of kojic acid using Aspergillus oryzae NRRL484

Yasushi Wakisaka, Takayuki Segawa, Koreyoshi Imamura, Takaharu Sakiyama, Kazuhiro Nakanishi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To apply membrane-surface liquid culture (MSLC), reported in previous papers (J. Ferment. Bioeng., 80: 35-40, 41-46, 1995) to large-scale production, a novel bioreactor was constructed in which molds were grown on the outer surface of a cylindrical porous membrane facing the air with liquid medium running down along its inner surface. We cultivated Aspergillus oryzae NRRL484 to produce kojic acid as a model secondary metabolite. An SE20 membrane composed of polysulfone with a nominal pore diameter of 0.2 μm (Fuji Photo Film Co.) was found to be most suitable based on the facts that the mass transfer through the membrane had no appreciable effect on the kojic acid production and the mycelia did not pass through the membrane pores. Kojic acid was produced at higher levels than in shaking flask culture. The highest kojic acid concentration in the batch MSLCs using the cylindrical membrane module with an active surface area of 220 cm2 was 14, 45, and 60 mg/ml for glucose concentrations in the medium of 5, 10, and 20%, respectively. In the shaking flask cultures using 10 and 20% glucose, the maximum kojic acid concentrations were 24 and 22 mg/ml, respectively. In the MSLC, the liquid medium was quite clear without any contamination of cells. Furthermore, the mold was highly stable against autolysis, and continuous cultivation with holding cells on the membrane surface could be conducted for over 70 d. When the average feed rate of the medium containing 10% glucose and 0.1% yeast extract was 30 ml/d, the steady-state kojic acid concentration at the outlet of the MSLC apparatus was in the range of 45-50 mg/ml.

Original languageEnglish
Pages (from-to)488-494
Number of pages7
JournalJournal of Fermentation and Bioengineering
Volume85
Issue number5
DOIs
Publication statusPublished - 1998

Fingerprint

Aspergillus oryzae
Aspergillus
Membranes
Acids
Liquids
Glucose
Fungi
Autolysis
kojic acid
Mycelium
Bioreactors
Polysulfones
Motion Pictures
Molds
Metabolites
Yeast
Yeasts
Air
Cell Membrane
Contamination

Keywords

  • Bioreactor
  • Kojic acid
  • Membrane-surface liquid culture
  • Mold

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Development of a cylindrical apparatus for membrane-surface liquid culture and production of kojic acid using Aspergillus oryzae NRRL484. / Wakisaka, Yasushi; Segawa, Takayuki; Imamura, Koreyoshi; Sakiyama, Takaharu; Nakanishi, Kazuhiro.

In: Journal of Fermentation and Bioengineering, Vol. 85, No. 5, 1998, p. 488-494.

Research output: Contribution to journalArticle

@article{a69338f667fe4a20ba6f6c507350d6c9,
title = "Development of a cylindrical apparatus for membrane-surface liquid culture and production of kojic acid using Aspergillus oryzae NRRL484",
abstract = "To apply membrane-surface liquid culture (MSLC), reported in previous papers (J. Ferment. Bioeng., 80: 35-40, 41-46, 1995) to large-scale production, a novel bioreactor was constructed in which molds were grown on the outer surface of a cylindrical porous membrane facing the air with liquid medium running down along its inner surface. We cultivated Aspergillus oryzae NRRL484 to produce kojic acid as a model secondary metabolite. An SE20 membrane composed of polysulfone with a nominal pore diameter of 0.2 μm (Fuji Photo Film Co.) was found to be most suitable based on the facts that the mass transfer through the membrane had no appreciable effect on the kojic acid production and the mycelia did not pass through the membrane pores. Kojic acid was produced at higher levels than in shaking flask culture. The highest kojic acid concentration in the batch MSLCs using the cylindrical membrane module with an active surface area of 220 cm2 was 14, 45, and 60 mg/ml for glucose concentrations in the medium of 5, 10, and 20{\%}, respectively. In the shaking flask cultures using 10 and 20{\%} glucose, the maximum kojic acid concentrations were 24 and 22 mg/ml, respectively. In the MSLC, the liquid medium was quite clear without any contamination of cells. Furthermore, the mold was highly stable against autolysis, and continuous cultivation with holding cells on the membrane surface could be conducted for over 70 d. When the average feed rate of the medium containing 10{\%} glucose and 0.1{\%} yeast extract was 30 ml/d, the steady-state kojic acid concentration at the outlet of the MSLC apparatus was in the range of 45-50 mg/ml.",
keywords = "Bioreactor, Kojic acid, Membrane-surface liquid culture, Mold",
author = "Yasushi Wakisaka and Takayuki Segawa and Koreyoshi Imamura and Takaharu Sakiyama and Kazuhiro Nakanishi",
year = "1998",
doi = "10.1016/S0922-338X(98)80067-6",
language = "English",
volume = "85",
pages = "488--494",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "The Society for Biotechnology, Japan",
number = "5",

}

TY - JOUR

T1 - Development of a cylindrical apparatus for membrane-surface liquid culture and production of kojic acid using Aspergillus oryzae NRRL484

AU - Wakisaka, Yasushi

AU - Segawa, Takayuki

AU - Imamura, Koreyoshi

AU - Sakiyama, Takaharu

AU - Nakanishi, Kazuhiro

PY - 1998

Y1 - 1998

N2 - To apply membrane-surface liquid culture (MSLC), reported in previous papers (J. Ferment. Bioeng., 80: 35-40, 41-46, 1995) to large-scale production, a novel bioreactor was constructed in which molds were grown on the outer surface of a cylindrical porous membrane facing the air with liquid medium running down along its inner surface. We cultivated Aspergillus oryzae NRRL484 to produce kojic acid as a model secondary metabolite. An SE20 membrane composed of polysulfone with a nominal pore diameter of 0.2 μm (Fuji Photo Film Co.) was found to be most suitable based on the facts that the mass transfer through the membrane had no appreciable effect on the kojic acid production and the mycelia did not pass through the membrane pores. Kojic acid was produced at higher levels than in shaking flask culture. The highest kojic acid concentration in the batch MSLCs using the cylindrical membrane module with an active surface area of 220 cm2 was 14, 45, and 60 mg/ml for glucose concentrations in the medium of 5, 10, and 20%, respectively. In the shaking flask cultures using 10 and 20% glucose, the maximum kojic acid concentrations were 24 and 22 mg/ml, respectively. In the MSLC, the liquid medium was quite clear without any contamination of cells. Furthermore, the mold was highly stable against autolysis, and continuous cultivation with holding cells on the membrane surface could be conducted for over 70 d. When the average feed rate of the medium containing 10% glucose and 0.1% yeast extract was 30 ml/d, the steady-state kojic acid concentration at the outlet of the MSLC apparatus was in the range of 45-50 mg/ml.

AB - To apply membrane-surface liquid culture (MSLC), reported in previous papers (J. Ferment. Bioeng., 80: 35-40, 41-46, 1995) to large-scale production, a novel bioreactor was constructed in which molds were grown on the outer surface of a cylindrical porous membrane facing the air with liquid medium running down along its inner surface. We cultivated Aspergillus oryzae NRRL484 to produce kojic acid as a model secondary metabolite. An SE20 membrane composed of polysulfone with a nominal pore diameter of 0.2 μm (Fuji Photo Film Co.) was found to be most suitable based on the facts that the mass transfer through the membrane had no appreciable effect on the kojic acid production and the mycelia did not pass through the membrane pores. Kojic acid was produced at higher levels than in shaking flask culture. The highest kojic acid concentration in the batch MSLCs using the cylindrical membrane module with an active surface area of 220 cm2 was 14, 45, and 60 mg/ml for glucose concentrations in the medium of 5, 10, and 20%, respectively. In the shaking flask cultures using 10 and 20% glucose, the maximum kojic acid concentrations were 24 and 22 mg/ml, respectively. In the MSLC, the liquid medium was quite clear without any contamination of cells. Furthermore, the mold was highly stable against autolysis, and continuous cultivation with holding cells on the membrane surface could be conducted for over 70 d. When the average feed rate of the medium containing 10% glucose and 0.1% yeast extract was 30 ml/d, the steady-state kojic acid concentration at the outlet of the MSLC apparatus was in the range of 45-50 mg/ml.

KW - Bioreactor

KW - Kojic acid

KW - Membrane-surface liquid culture

KW - Mold

UR - http://www.scopus.com/inward/record.url?scp=0031799953&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031799953&partnerID=8YFLogxK

U2 - 10.1016/S0922-338X(98)80067-6

DO - 10.1016/S0922-338X(98)80067-6

M3 - Article

VL - 85

SP - 488

EP - 494

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 5

ER -