Abstract
Currently-used rodent tumor models, including transgenic tumor models, or subcutaneously growing tumors in mice, do not sufficiently represent clinical cancer. We report here development of methods to obtain a highly clinically-accurate rectal cancer model. This model was established by intrarectal transplantation of mouse rectal cancer cells, stably expressing green fluorescent protein (GFP), followed by disrupting the epithelial cell layer of the rectal mucosa by instilling an acetic acid solution. Early-stage tumor was detected in the rectal mucosa by 6 days after transplantation. The tumor then became invasive into the submucosal tissue. The tumor incidence was 100% and mean volume (±SD) was 1232.4 ± 994.7 mm3 at 4 weeks after transplantation detected by fluorescence imaging. Spontaneous lymph node metastasis and lung metastasis were also found approximately 4 weeks after transplantation in over 90% of mice. This rectal tumor model precisely mimics the natural history of rectal cancer and can be used to study early tumor development, metastasis, and discovery and evaluation of novel therapeutics for this treatment-resistant disease.
| Original language | English |
|---|---|
| Article number | e79453 |
| Journal | PLoS One |
| Volume | 8 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Nov 12 2013 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
Development of a clinically-precise mouse model of rectal cancer. / Kishimoto, Hiroyuki; Momiyama, Masashi; Aki, Ryoichi; Kimura, Hiroaki; Suetsugu, Atsushi; Bouvet, Michael; Fujiwara, Toshiyoshi; Hoffman, Robert M.
In: PLoS One, Vol. 8, No. 11, e79453, 12.11.2013.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of a clinically-precise mouse model of rectal cancer
AU - Kishimoto, Hiroyuki
AU - Momiyama, Masashi
AU - Aki, Ryoichi
AU - Kimura, Hiroaki
AU - Suetsugu, Atsushi
AU - Bouvet, Michael
AU - Fujiwara, Toshiyoshi
AU - Hoffman, Robert M.
PY - 2013/11/12
Y1 - 2013/11/12
N2 - Currently-used rodent tumor models, including transgenic tumor models, or subcutaneously growing tumors in mice, do not sufficiently represent clinical cancer. We report here development of methods to obtain a highly clinically-accurate rectal cancer model. This model was established by intrarectal transplantation of mouse rectal cancer cells, stably expressing green fluorescent protein (GFP), followed by disrupting the epithelial cell layer of the rectal mucosa by instilling an acetic acid solution. Early-stage tumor was detected in the rectal mucosa by 6 days after transplantation. The tumor then became invasive into the submucosal tissue. The tumor incidence was 100% and mean volume (±SD) was 1232.4 ± 994.7 mm3 at 4 weeks after transplantation detected by fluorescence imaging. Spontaneous lymph node metastasis and lung metastasis were also found approximately 4 weeks after transplantation in over 90% of mice. This rectal tumor model precisely mimics the natural history of rectal cancer and can be used to study early tumor development, metastasis, and discovery and evaluation of novel therapeutics for this treatment-resistant disease.
AB - Currently-used rodent tumor models, including transgenic tumor models, or subcutaneously growing tumors in mice, do not sufficiently represent clinical cancer. We report here development of methods to obtain a highly clinically-accurate rectal cancer model. This model was established by intrarectal transplantation of mouse rectal cancer cells, stably expressing green fluorescent protein (GFP), followed by disrupting the epithelial cell layer of the rectal mucosa by instilling an acetic acid solution. Early-stage tumor was detected in the rectal mucosa by 6 days after transplantation. The tumor then became invasive into the submucosal tissue. The tumor incidence was 100% and mean volume (±SD) was 1232.4 ± 994.7 mm3 at 4 weeks after transplantation detected by fluorescence imaging. Spontaneous lymph node metastasis and lung metastasis were also found approximately 4 weeks after transplantation in over 90% of mice. This rectal tumor model precisely mimics the natural history of rectal cancer and can be used to study early tumor development, metastasis, and discovery and evaluation of novel therapeutics for this treatment-resistant disease.
UR - http://www.scopus.com/inward/record.url?scp=84896719129&partnerID=8YFLogxK
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U2 - 10.1371/journal.pone.0079453
DO - 10.1371/journal.pone.0079453
M3 - Article
C2 - 24265772
AN - SCOPUS:84896719129
VL - 8
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 11
M1 - e79453
ER -