Development of a bifunctional immunoliposome system for combined drug delivery and imaging in vivo

Bin Feng, Kazuhito Tomizawa, Hiroyuki Michiue, Xiao Jian Han, Shin ichi Miyatake, Hideki Matsui

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The diverse characteristics of immunoliposomes provide advantages for utilization in drug delivery systems. In this study, we fused the antibody affinity motif of protein A (ZZ) with Gaussia luciferase (GLase). The fused protein conjugated with an anti-epidermal growth factor receptor (EGFR) monoclonal antibody (GLase-ZZ-His-mAb) was effectively delivered into glioma cells expressing an activated EGFR mutant (EGFRvIII) and the bioluminescence was visualized in the cells. Immunoliposomes were further constructed with DSPE-PEG-MAL for covalent GLase-ZZ-His-mAb conjugation. A fluorescence dye (HPTS) encapsulated in immunoliposomes conjugated with GLase-ZZ-His-mAb was effectively delivered into EGFRvIII-expressing glioma cells. In a murine xenograft model of glioma, moreover, specific targeting of the immunoliposomes was visualized in the tumor. This new bifunctional immunoliposome system has the potential for drug delivery and imaging in vivo.

Original languageEnglish
Pages (from-to)4139-4145
Number of pages7
JournalBiomaterials
Volume31
Issue number14
DOIs
Publication statusPublished - May 2010

Fingerprint

Drug Delivery Systems
Luciferases
Drug delivery
Glioma
Bioluminescence
Proteins
Imaging techniques
Monoclonal antibodies
Epidermal Growth Factor Receptor
Antibodies
Polyethylene glycols
Tumors
Dyes
Fluorescence
Cells
Antibody Affinity
Staphylococcal Protein A
Heterografts
Coloring Agents
Monoclonal Antibodies

Keywords

  • Bioluminescence
  • Boron neutron capture therapy
  • EGFR
  • Gaussia luciferase
  • Glioma cells
  • Immunoliposome

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Development of a bifunctional immunoliposome system for combined drug delivery and imaging in vivo. / Feng, Bin; Tomizawa, Kazuhito; Michiue, Hiroyuki; Han, Xiao Jian; Miyatake, Shin ichi; Matsui, Hideki.

In: Biomaterials, Vol. 31, No. 14, 05.2010, p. 4139-4145.

Research output: Contribution to journalArticle

Feng, Bin ; Tomizawa, Kazuhito ; Michiue, Hiroyuki ; Han, Xiao Jian ; Miyatake, Shin ichi ; Matsui, Hideki. / Development of a bifunctional immunoliposome system for combined drug delivery and imaging in vivo. In: Biomaterials. 2010 ; Vol. 31, No. 14. pp. 4139-4145.
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