Development of a bifunctional immunoliposome system for combined drug delivery and imaging in vivo

Bin Feng; Kazuhito Tomizawa; Hiroyuki Michiue; Xiao Jian Han; Shin ichi Miyatake; Hideki Matsui

doi:10.1016/j.biomaterials.2010.01.086

Development of a bifunctional immunoliposome system for combined drug delivery and imaging in vivo

Bin Feng, Kazuhito Tomizawa, Hiroyuki Michiue, Xiao Jian Han, Shin ichi Miyatake, Hideki Matsui

Neutron Therapy Research Center

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

The diverse characteristics of immunoliposomes provide advantages for utilization in drug delivery systems. In this study, we fused the antibody affinity motif of protein A (ZZ) with Gaussia luciferase (GLase). The fused protein conjugated with an anti-epidermal growth factor receptor (EGFR) monoclonal antibody (GLase-ZZ-His-mAb) was effectively delivered into glioma cells expressing an activated EGFR mutant (EGFRvIII) and the bioluminescence was visualized in the cells. Immunoliposomes were further constructed with DSPE-PEG-MAL for covalent GLase-ZZ-His-mAb conjugation. A fluorescence dye (HPTS) encapsulated in immunoliposomes conjugated with GLase-ZZ-His-mAb was effectively delivered into EGFRvIII-expressing glioma cells. In a murine xenograft model of glioma, moreover, specific targeting of the immunoliposomes was visualized in the tumor. This new bifunctional immunoliposome system has the potential for drug delivery and imaging in vivo.

Original language	English
Pages (from-to)	4139-4145
Number of pages	7
Journal	Biomaterials
Volume	31
Issue number	14
DOIs	https://doi.org/10.1016/j.biomaterials.2010.01.086
Publication status	Published - May 2010

Keywords

Bioluminescence
Boron neutron capture therapy
EGFR
Gaussia luciferase
Glioma cells
Immunoliposome

ASJC Scopus subject areas

Bioengineering
Ceramics and Composites
Biophysics
Biomaterials
Mechanics of Materials

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10.1016/j.biomaterials.2010.01.086

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title = "Development of a bifunctional immunoliposome system for combined drug delivery and imaging in vivo",

abstract = "The diverse characteristics of immunoliposomes provide advantages for utilization in drug delivery systems. In this study, we fused the antibody affinity motif of protein A (ZZ) with Gaussia luciferase (GLase). The fused protein conjugated with an anti-epidermal growth factor receptor (EGFR) monoclonal antibody (GLase-ZZ-His-mAb) was effectively delivered into glioma cells expressing an activated EGFR mutant (EGFRvIII) and the bioluminescence was visualized in the cells. Immunoliposomes were further constructed with DSPE-PEG-MAL for covalent GLase-ZZ-His-mAb conjugation. A fluorescence dye (HPTS) encapsulated in immunoliposomes conjugated with GLase-ZZ-His-mAb was effectively delivered into EGFRvIII-expressing glioma cells. In a murine xenograft model of glioma, moreover, specific targeting of the immunoliposomes was visualized in the tumor. This new bifunctional immunoliposome system has the potential for drug delivery and imaging in vivo.",

keywords = "Bioluminescence, Boron neutron capture therapy, EGFR, Gaussia luciferase, Glioma cells, Immunoliposome",

author = "Bin Feng and Kazuhito Tomizawa and Hiroyuki Michiue and Han, {Xiao Jian} and Miyatake, {Shin ichi} and Hideki Matsui",

note = "Funding Information: This work was supported by a Grant-in-aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan and by a Grant-in-aid for Scientific Research from the Ministry of Health, Labour and Welfare of Japan . ",

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doi = "10.1016/j.biomaterials.2010.01.086",

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AU - Michiue, Hiroyuki

AU - Han, Xiao Jian

AU - Miyatake, Shin ichi

AU - Matsui, Hideki

N1 - Funding Information: This work was supported by a Grant-in-aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan and by a Grant-in-aid for Scientific Research from the Ministry of Health, Labour and Welfare of Japan .

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N2 - The diverse characteristics of immunoliposomes provide advantages for utilization in drug delivery systems. In this study, we fused the antibody affinity motif of protein A (ZZ) with Gaussia luciferase (GLase). The fused protein conjugated with an anti-epidermal growth factor receptor (EGFR) monoclonal antibody (GLase-ZZ-His-mAb) was effectively delivered into glioma cells expressing an activated EGFR mutant (EGFRvIII) and the bioluminescence was visualized in the cells. Immunoliposomes were further constructed with DSPE-PEG-MAL for covalent GLase-ZZ-His-mAb conjugation. A fluorescence dye (HPTS) encapsulated in immunoliposomes conjugated with GLase-ZZ-His-mAb was effectively delivered into EGFRvIII-expressing glioma cells. In a murine xenograft model of glioma, moreover, specific targeting of the immunoliposomes was visualized in the tumor. This new bifunctional immunoliposome system has the potential for drug delivery and imaging in vivo.

AB - The diverse characteristics of immunoliposomes provide advantages for utilization in drug delivery systems. In this study, we fused the antibody affinity motif of protein A (ZZ) with Gaussia luciferase (GLase). The fused protein conjugated with an anti-epidermal growth factor receptor (EGFR) monoclonal antibody (GLase-ZZ-His-mAb) was effectively delivered into glioma cells expressing an activated EGFR mutant (EGFRvIII) and the bioluminescence was visualized in the cells. Immunoliposomes were further constructed with DSPE-PEG-MAL for covalent GLase-ZZ-His-mAb conjugation. A fluorescence dye (HPTS) encapsulated in immunoliposomes conjugated with GLase-ZZ-His-mAb was effectively delivered into EGFRvIII-expressing glioma cells. In a murine xenograft model of glioma, moreover, specific targeting of the immunoliposomes was visualized in the tumor. This new bifunctional immunoliposome system has the potential for drug delivery and imaging in vivo.

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KW - Boron neutron capture therapy

KW - EGFR

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KW - Glioma cells

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Development of a bifunctional immunoliposome system for combined drug delivery and imaging in vivo

Abstract

Keywords

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