Development of a bifunctional immunoliposome system for combined drug delivery and imaging in vivo

Bin Feng, Kazuhito Tomizawa, Hiroyuki Michiue, Xiao Jian Han, Shin ichi Miyatake, Hideki Matsui

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The diverse characteristics of immunoliposomes provide advantages for utilization in drug delivery systems. In this study, we fused the antibody affinity motif of protein A (ZZ) with Gaussia luciferase (GLase). The fused protein conjugated with an anti-epidermal growth factor receptor (EGFR) monoclonal antibody (GLase-ZZ-His-mAb) was effectively delivered into glioma cells expressing an activated EGFR mutant (EGFRvIII) and the bioluminescence was visualized in the cells. Immunoliposomes were further constructed with DSPE-PEG-MAL for covalent GLase-ZZ-His-mAb conjugation. A fluorescence dye (HPTS) encapsulated in immunoliposomes conjugated with GLase-ZZ-His-mAb was effectively delivered into EGFRvIII-expressing glioma cells. In a murine xenograft model of glioma, moreover, specific targeting of the immunoliposomes was visualized in the tumor. This new bifunctional immunoliposome system has the potential for drug delivery and imaging in vivo.

Original languageEnglish
Pages (from-to)4139-4145
Number of pages7
JournalBiomaterials
Volume31
Issue number14
DOIs
Publication statusPublished - May 1 2010

Keywords

  • Bioluminescence
  • Boron neutron capture therapy
  • EGFR
  • Gaussia luciferase
  • Glioma cells
  • Immunoliposome

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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