Development of 5006 full-length CDNAs in barley

A tool for accessing cereal genomics resources

Kazuhiro Sato, Tadasu Shin-I, Motoaki Seki, Kazuo Shinozaki, Hideya Yoshida, Kazuyoshi Takeda, Yukiko Yamazaki, Matthieu Conte, Yuji Kohara

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

A collection of 5006 full-length (FL) cDNA sequences was developed in barley. Fifteen mRNA samples from various organs and treatments were pooled to develop a cDNA library using the CAP trapper method. More than 60 of the clones were confirmed to have complete coding sequences, based on comparison with rice amino acid and UniProt sequences. Blastn homologies (E

Original languageEnglish
Pages (from-to)81-89
Number of pages9
JournalDNA Research
Volume16
Issue number2
DOIs
Publication statusPublished - Apr 2009

Fingerprint

Hordeum
Genomics
Gene Library
Amino Acid Sequence
Complementary DNA
Clone Cells
Messenger RNA
Oryza
Edible Grain

Keywords

  • Full-length cDNA
  • Gene ontology
  • Hordeum vulgare
  • MRNA

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Medicine(all)

Cite this

Sato, K., Shin-I, T., Seki, M., Shinozaki, K., Yoshida, H., Takeda, K., ... Kohara, Y. (2009). Development of 5006 full-length CDNAs in barley: A tool for accessing cereal genomics resources. DNA Research, 16(2), 81-89. https://doi.org/10.1093/dnares/dsn034

Development of 5006 full-length CDNAs in barley : A tool for accessing cereal genomics resources. / Sato, Kazuhiro; Shin-I, Tadasu; Seki, Motoaki; Shinozaki, Kazuo; Yoshida, Hideya; Takeda, Kazuyoshi; Yamazaki, Yukiko; Conte, Matthieu; Kohara, Yuji.

In: DNA Research, Vol. 16, No. 2, 04.2009, p. 81-89.

Research output: Contribution to journalArticle

Sato, K, Shin-I, T, Seki, M, Shinozaki, K, Yoshida, H, Takeda, K, Yamazaki, Y, Conte, M & Kohara, Y 2009, 'Development of 5006 full-length CDNAs in barley: A tool for accessing cereal genomics resources', DNA Research, vol. 16, no. 2, pp. 81-89. https://doi.org/10.1093/dnares/dsn034
Sato, Kazuhiro ; Shin-I, Tadasu ; Seki, Motoaki ; Shinozaki, Kazuo ; Yoshida, Hideya ; Takeda, Kazuyoshi ; Yamazaki, Yukiko ; Conte, Matthieu ; Kohara, Yuji. / Development of 5006 full-length CDNAs in barley : A tool for accessing cereal genomics resources. In: DNA Research. 2009 ; Vol. 16, No. 2. pp. 81-89.
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