Development of 16S rDNA-based PCR assay for detecting Centipeda periodontii and Selenomonas sputigena

S. Sawada, Susumu Kokeguchi, Shogo Takashiba, Y. Murayama

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

S. SAWADA, S. KOKEGUCHI, S. TAKASHIBA AND Y. MURAYAMA. 2000. To detect oral motile bacteria directly from dental plaque, specific PCR primers for Centipeda periodontii and Selenomonas sputigena were designed based on the sequence analysis of their 16S rDNA. The primers were specific and sensitive enough to amplify DNA fragments from the available oral bacteria. The detection limit was fewer than 10 bacterial cells per sample. It was also possible to detect these bacteria in dental plaque. The prevalence of these bacteria varied in each sample. The specific primers designed in this study may clarify the epidemiology of periodontal disease.

Original languageEnglish
Pages (from-to)423-426
Number of pages4
JournalLetters in Applied Microbiology
Volume30
Issue number6
Publication statusPublished - 2000

Fingerprint

Selenomonas
Ribosomal DNA
Assays
Bacteria
assay
Dental Plaque
Polymerase Chain Reaction
bacterium
Epidemiology
Periodontal Diseases
epidemiology
Sequence Analysis
Limit of Detection
DNA

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Water Science and Technology
  • Fluid Flow and Transfer Processes
  • Microbiology
  • Ecology

Cite this

Development of 16S rDNA-based PCR assay for detecting Centipeda periodontii and Selenomonas sputigena. / Sawada, S.; Kokeguchi, Susumu; Takashiba, Shogo; Murayama, Y.

In: Letters in Applied Microbiology, Vol. 30, No. 6, 2000, p. 423-426.

Research output: Contribution to journalArticle

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