Development of 16S rDNA-based PCR assay for detecting Centipeda periodontii and Selenomonas sputigena

S. Sawada, S. Kokeguchi, S. Takashiba, Y. Murayama

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

S. SAWADA, S. KOKEGUCHI, S. TAKASHIBA AND Y. MURAYAMA. 2000. To detect oral motile bacteria directly from dental plaque, specific PCR primers for Centipeda periodontii and Selenomonas sputigena were designed based on the sequence analysis of their 16S rDNA. The primers were specific and sensitive enough to amplify DNA fragments from the available oral bacteria. The detection limit was fewer than 10 bacterial cells per sample. It was also possible to detect these bacteria in dental plaque. The prevalence of these bacteria varied in each sample. The specific primers designed in this study may clarify the epidemiology of periodontal disease.

Original languageEnglish
Pages (from-to)423-426
Number of pages4
JournalLetters in Applied Microbiology
Volume30
Issue number6
DOIs
Publication statusPublished - 2000

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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