TY - JOUR
T1 - Development of 16S rDNA-based PCR assay for detecting Centipeda periodontii and Selenomonas sputigena
AU - Sawada, S.
AU - Kokeguchi, S.
AU - Takashiba, S.
AU - Murayama, Y.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2000
Y1 - 2000
N2 - S. SAWADA, S. KOKEGUCHI, S. TAKASHIBA AND Y. MURAYAMA. 2000. To detect oral motile bacteria directly from dental plaque, specific PCR primers for Centipeda periodontii and Selenomonas sputigena were designed based on the sequence analysis of their 16S rDNA. The primers were specific and sensitive enough to amplify DNA fragments from the available oral bacteria. The detection limit was fewer than 10 bacterial cells per sample. It was also possible to detect these bacteria in dental plaque. The prevalence of these bacteria varied in each sample. The specific primers designed in this study may clarify the epidemiology of periodontal disease.
AB - S. SAWADA, S. KOKEGUCHI, S. TAKASHIBA AND Y. MURAYAMA. 2000. To detect oral motile bacteria directly from dental plaque, specific PCR primers for Centipeda periodontii and Selenomonas sputigena were designed based on the sequence analysis of their 16S rDNA. The primers were specific and sensitive enough to amplify DNA fragments from the available oral bacteria. The detection limit was fewer than 10 bacterial cells per sample. It was also possible to detect these bacteria in dental plaque. The prevalence of these bacteria varied in each sample. The specific primers designed in this study may clarify the epidemiology of periodontal disease.
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U2 - 10.1046/j.1472-765x.2000.00735.x
DO - 10.1046/j.1472-765x.2000.00735.x
M3 - Article
C2 - 10849269
AN - SCOPUS:0034130659
SN - 0266-8254
VL - 30
SP - 423
EP - 426
JO - Letters in Applied Microbiology
JF - Letters in Applied Microbiology
IS - 6
ER -