Development and characterization of recombinant chromosome substitution lines (RCSLs) using Hordeum vulgare subsp. spontaneum as a source of donor alleles in a Hordeum vulgare subsp. vulgare background

I. Matus, A. Corey, T. Filichkin, P. M. Hayes, M. I. Vales, J. Kling, O. Riera-Lizarazu, Kazuhiro Sato, W. Powell, R. Waugh

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

The ancestor of barley (Hordeum vulgare subsp. spontaneum) may be a source of novel alleles for crop improvement. We developed a set of recombinant chromosome substitution lines (RCSLs) using an accession of H. vulgare subsp. spontaneum (Caesarea 26-24, from Israel) as the donor and Hordeum vulgare subsp. vulgare 'Harrington' (the North American malting quality standard) as the recurrent parent via two backcrosses to the recurrent parent, followed by six generations of selfing. Here we report (i) the genomic architecture of the RCSLs, as inferred by simple sequence repeat (SSR) markers, and (ii) the effects of H. vulgare subsp. spontaneum genome segment introgressions in terms of three classes of phenotypes: inflorescence yield components, malting quality traits, and domestication traits. Significant differences among the RCSLs were detected for all phenotypes measured. The phenotypic effects of the introgressions were assessed using association analysis, and these were referenced to quantitative trait loci (QTL) reported in the literature. Hordeum vulgare subsp. spontaneum, despite its overall inferior phenotype, contributed some favorable alleles for agronomic and malting quality traits. In most cases, the introgression of the ancestral genome resulted in a loss of desirable phenotypes in the cultivated parent. Although disappointing from a plant breeding perspective, this finding may prove to be a useful tool for gene discovery.

Original languageEnglish
Pages (from-to)1010-1023
Number of pages14
JournalGenome
Volume46
Issue number6
DOIs
Publication statusPublished - Dec 2003

Fingerprint

Hordeum vulgare subsp. vulgare
Hordeum vulgare subsp. spontaneum
substitution lines
Hordeum
malting quality
Chromosomes
Alleles
introgression
alleles
phenotype
Phenotype
genome
domestication
selfing
plant breeding
Genome
yield components
Israel
Inflorescence
quantitative trait loci

Keywords

  • Association analysis
  • Genetic resources
  • Hordeum vulgare subsp spontaneum
  • Hordeum vulgare subsp. vulgare
  • QTLs

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biotechnology
  • Genetics
  • Genetics(clinical)

Cite this

Development and characterization of recombinant chromosome substitution lines (RCSLs) using Hordeum vulgare subsp. spontaneum as a source of donor alleles in a Hordeum vulgare subsp. vulgare background. / Matus, I.; Corey, A.; Filichkin, T.; Hayes, P. M.; Vales, M. I.; Kling, J.; Riera-Lizarazu, O.; Sato, Kazuhiro; Powell, W.; Waugh, R.

In: Genome, Vol. 46, No. 6, 12.2003, p. 1010-1023.

Research output: Contribution to journalArticle

Matus, I. ; Corey, A. ; Filichkin, T. ; Hayes, P. M. ; Vales, M. I. ; Kling, J. ; Riera-Lizarazu, O. ; Sato, Kazuhiro ; Powell, W. ; Waugh, R. / Development and characterization of recombinant chromosome substitution lines (RCSLs) using Hordeum vulgare subsp. spontaneum as a source of donor alleles in a Hordeum vulgare subsp. vulgare background. In: Genome. 2003 ; Vol. 46, No. 6. pp. 1010-1023.
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AU - Corey, A.

AU - Filichkin, T.

AU - Hayes, P. M.

AU - Vales, M. I.

AU - Kling, J.

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AU - Sato, Kazuhiro

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