Development and characterization of a silica monolith immobilized enzyme micro-bioreactor

Koei Kawakami, Yoshihide Sera, Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Several 10-cm-long capillary tubes [made of poly(ether ether ketone) (PEEK)] with inside diameters of 0.1-2.0 mm were filled with silica monolith-immobilized protease derived by in situ sol-gel transition from a 1:4 mixture of tetramethoxysilane and methyltrimethoxysilane. Transesterification between 20 mM (S)-(-)-glycidol and 0.4 M vinyl n-butyrate in an organic solvent was used as the test reaction. The substrate solution flowed through the column at a flow rate of 0.0004-5.0 mL·min-1. The conversion in the micro-bioreactor was higher than that in the batch reactor at a high liquid flow rate. When three tubes were connected in series, the conversion at a fixed ratio of the mass of the enzyme to the liquid flow rate was increased by approximately 50%, because of the tripling of the flow rate as compared to the case with a single tube. Changes in the tube diameter had no influence on the conversion at a fixed superficial liquid velocity. Further, the conversion increased with a decrease in the enzyme content. These results were ascribed to the apparent effect of liquid-solid mass transfer and were analyzed quantitatively using a simple mathematical model.

Original languageEnglish
Pages (from-to)236-240
Number of pages5
JournalIndustrial and Engineering Chemistry Research
Volume44
Issue number1
Publication statusPublished - Jan 5 2005
Externally publishedYes

Fingerprint

Immobilized Enzymes
Bioreactors
Silicon Dioxide
bioreactor
Enzymes
silica
Silica
Flow rate
enzyme
glycidol
liquid
Liquids
ether
Capillary tubes
Polyether ether ketones
Butyrates
Transesterification
Batch reactors
ketone
Organic solvents

ASJC Scopus subject areas

  • Polymers and Plastics
  • Environmental Science(all)
  • Chemical Engineering (miscellaneous)

Cite this

Development and characterization of a silica monolith immobilized enzyme micro-bioreactor. / Kawakami, Koei; Sera, Yoshihide; Sakai, Shinji; Ono, Tsutomu; Ijima, Hiroyuki.

In: Industrial and Engineering Chemistry Research, Vol. 44, No. 1, 05.01.2005, p. 236-240.

Research output: Contribution to journalArticle

Kawakami, Koei ; Sera, Yoshihide ; Sakai, Shinji ; Ono, Tsutomu ; Ijima, Hiroyuki. / Development and characterization of a silica monolith immobilized enzyme micro-bioreactor. In: Industrial and Engineering Chemistry Research. 2005 ; Vol. 44, No. 1. pp. 236-240.
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