Detoxification of 7-dehydrocholesterol fatal to Helicobacter pylori is a novel role of cholesterol glucosylation

Hirofumi Shimomura, Kouichi Hosoda, David J. Mcgee, Shunji Hayashi, Kenji Yokota, Yoshikazu Hiraia

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The glucosylation of free cholesterol (FC) by Helicobacter pylori cells has various biological significances for the survival of this bacterium. H. pylori cells with glucosylated FC are capable of evading host immune systems, such as phagocytosis by macrophages and activation of antigen-specific T cells, and surviving in the gastric mucosal tissues for long periods. An additional role of cholesterol glucosylation in the survival of H. pylori which is distinct from the role of escaping the host immune system, however, has yet to be identified. This study demonstrated that 7-dehydrocholesterol (7dFC), an FC precursor, is a toxic compound fatal to H. pylori cells, but the cell membrane of H. pylori is capable of absorbing this toxic sterol via glucosylation. In contrast to the case with 7dFC, no toxicity to H. pylori cells was detected from the glucosylated 7dFC. In addition, cgt gene mutant H. pylori cells that cannot glucosylate cholesterols had higher susceptibility to the toxic action of 7dFC than wild-type H. pylori cells. These results indicate that the cgt gene product of H. pylori serves to detoxify the sterol fatal to this bacterium and to permit this toxic sterol as a cell membrane lipid component. In summary, this study defined a novel role of cholesterol glucosylation in H. pylori.

Original languageEnglish
Pages (from-to)359-367
Number of pages9
JournalJournal of Bacteriology
Volume195
Issue number2
DOIs
Publication statusPublished - Jan 2013

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Helicobacter pylori
Cholesterol
Poisons
Sterols
Immune System
7-dehydrocholesterol
Bacteria
Toxic Actions
Macrophage Activation
Membrane Lipids
Phagocytosis
Genes
Stomach
Mucous Membrane
Cell Membrane
T-Lymphocytes
Antigens

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Detoxification of 7-dehydrocholesterol fatal to Helicobacter pylori is a novel role of cholesterol glucosylation. / Shimomura, Hirofumi; Hosoda, Kouichi; Mcgee, David J.; Hayashi, Shunji; Yokota, Kenji; Hiraia, Yoshikazu.

In: Journal of Bacteriology, Vol. 195, No. 2, 01.2013, p. 359-367.

Research output: Contribution to journalArticle

Shimomura, Hirofumi ; Hosoda, Kouichi ; Mcgee, David J. ; Hayashi, Shunji ; Yokota, Kenji ; Hiraia, Yoshikazu. / Detoxification of 7-dehydrocholesterol fatal to Helicobacter pylori is a novel role of cholesterol glucosylation. In: Journal of Bacteriology. 2013 ; Vol. 195, No. 2. pp. 359-367.
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