Determination of polyamines by capillary electrophoresis using salicylaldehyde-5-sulfonate as a derivatizing reagent

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Abstract

Here we describe a protocol for the determination of polyamines using capillary electrophoresis with ultraviolet absorbance detection. Aliphatic polyamines were derivatized with salicylaldehyde-5-sulfonate (SAS) which formed Schiff base with amino groups, resulting in anionic derivatives. The derivatization of polyamines, including putrescine (PUT), cadaverine (CAD), spermidine (SPD), and spermine (SPM), was conducted in 10 mM HEPES buffer with pH 7.8 containing 5 mM SAS in a mixed solvent of water and ethanol. The SAS derivatives were separated using a background electrolyte composed of 10mMphosphate buffer with pH 7.8. Calibration curves were linear over a concentration range of 20–200 μM for CAD, PUT, SPD, and SPM, and the limits of detection (LOD) were several μM for all polyamines. Furthermore, solid phase extraction was coupled with the CE method to improve the LOD to sub-μM levels, and the calibration curves were linear over a concentration range of 1–20 μM for CAD, PUT, and SPD.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages61-68
Number of pages8
Volume1694
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1694
ISSN (Print)1064-3745

Keywords

  • Capillary electrophoresis
  • Polyamines
  • Salicylaldehyde-5-sulfonate

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Kaneta, T. (2018). Determination of polyamines by capillary electrophoresis using salicylaldehyde-5-sulfonate as a derivatizing reagent. In Methods in Molecular Biology (Vol. 1694, pp. 61-68). (Methods in Molecular Biology; Vol. 1694). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7398-9_5