Determination of oxidized low-density lipoproteins (ox-LDL) versus ox-LDL/β2GPI complexes for the assessment of autoimmune-mediated atherosclerosis

Luis R. Lopez, Tammy R. Buckner, Beth L. Hurley, Kazuko Kobayashi, Eiji Matsuura

Research output: Chapter in Book/Report/Conference proceedingConference contribution

21 Citations (Scopus)

Abstract

The immunolocalization of oxidized low-density lipoproteins (ox-LDL), β2-glycoprotein I (β2GPI), CD4+/CD8+ immunoreactive lymphocytes, and immunoglobulins in atherosclerotic lesions strongly suggested an active participation of the immune system in atherogenesis. Oxidative stress leading to ox-LDL production is thought to play a central role in both the initiation and progression of atherosclerosis. ox-LDL is highly proinflammatory and chemotactic for macrophage/monocyte and immune cells. Enzyme-linked immunosorbent assays (ELISAs) to measure circulating ox-LDL have been developed and are being currently used to assess oxidative stress as risk factor or marker of atherosclerotic disease. ox-LDL interacts with β2GPI and circulating ox-LDL/β2GPI complexes have been demonstrated in patients with systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS). It has been postulated that β2GPI binds ox-LDL to neutralize its proinflammatory and proatherosclerotic effects. Because β2GPI is ubiquitous in plasma, its interaction with ox-LDL may mask oxidized epitopes recognized by capture antibodies potentially interfering with immunoassays results. The measurement of ox-LDL/β2GPI complexes may circumvent this interference representing a more physiological and accurate way of measuring ox-LDL.

Original languageEnglish
Title of host publicationAutoimmunity, Part A Basic Principles and New Diagnostic Tools
PublisherBlackwell Publishing Inc.
Pages303-310
Number of pages8
ISBN (Print)1573316636, 9781573316637
DOIs
Publication statusPublished - Aug 2007

Publication series

NameAnnals of the New York Academy of Sciences
Volume1109
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • Atherosclerosis
  • Autoimmunity
  • ELISA
  • Oxidative stress
  • Oxidized low-density lipoprotein (ox-LDL)
  • β2-glycoprotein I (βGPI)

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Fingerprint Dive into the research topics of 'Determination of oxidized low-density lipoproteins (ox-LDL) versus ox-LDL/β2GPI complexes for the assessment of autoimmune-mediated atherosclerosis'. Together they form a unique fingerprint.

  • Cite this

    Lopez, L. R., Buckner, T. R., Hurley, B. L., Kobayashi, K., & Matsuura, E. (2007). Determination of oxidized low-density lipoproteins (ox-LDL) versus ox-LDL/β2GPI complexes for the assessment of autoimmune-mediated atherosclerosis. In Autoimmunity, Part A Basic Principles and New Diagnostic Tools (pp. 303-310). (Annals of the New York Academy of Sciences; Vol. 1109). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1398.036