Determination of oxidized low-density lipoproteins (ox-LDL) versus ox-LDL/β2GPI complexes for the assessment of autoimmune-mediated atherosclerosis

Luis R. Lopez, Tammy R. Buckner, Beth L. Hurley, Kazuko Kobayashi, Eiji Matsuura

Research output: Chapter in Book/Report/Conference proceedingConference contribution

21 Citations (Scopus)

Abstract

The immunolocalization of oxidized low-density lipoproteins (ox-LDL), β2-glycoprotein I (β2GPI), CD4+/CD8+ immunoreactive lymphocytes, and immunoglobulins in atherosclerotic lesions strongly suggested an active participation of the immune system in atherogenesis. Oxidative stress leading to ox-LDL production is thought to play a central role in both the initiation and progression of atherosclerosis. ox-LDL is highly proinflammatory and chemotactic for macrophage/monocyte and immune cells. Enzyme-linked immunosorbent assays (ELISAs) to measure circulating ox-LDL have been developed and are being currently used to assess oxidative stress as risk factor or marker of atherosclerotic disease. ox-LDL interacts with β2GPI and circulating ox-LDL/β2GPI complexes have been demonstrated in patients with systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS). It has been postulated that β2GPI binds ox-LDL to neutralize its proinflammatory and proatherosclerotic effects. Because β2GPI is ubiquitous in plasma, its interaction with ox-LDL may mask oxidized epitopes recognized by capture antibodies potentially interfering with immunoassays results. The measurement of ox-LDL/β2GPI complexes may circumvent this interference representing a more physiological and accurate way of measuring ox-LDL.

Original languageEnglish
Title of host publicationAnnals of the New York Academy of Sciences
Pages303-310
Number of pages8
Volume1109
DOIs
Publication statusPublished - Aug 2007

Publication series

NameAnnals of the New York Academy of Sciences
Volume1109
ISSN (Print)00778923
ISSN (Electronic)17496632

Fingerprint

LDL Lipoproteins
Atherosclerosis
Glycoproteins
Oxidative stress
Oxidative Stress
oxidized low density lipoprotein
Plasma interactions
Immunosorbents
Antiphospholipid Syndrome
Lymphocytes
Macrophages
Immune system
Masks
Immunoassay
Systemic Lupus Erythematosus
Immunoglobulins
Epitopes
Monocytes
Immune System
Assays

Keywords

  • β2-glycoprotein I (βGPI)
  • Atherosclerosis
  • Autoimmunity
  • ELISA
  • Oxidative stress
  • Oxidized low-density lipoprotein (ox-LDL)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lopez, L. R., Buckner, T. R., Hurley, B. L., Kobayashi, K., & Matsuura, E. (2007). Determination of oxidized low-density lipoproteins (ox-LDL) versus ox-LDL/β2GPI complexes for the assessment of autoimmune-mediated atherosclerosis. In Annals of the New York Academy of Sciences (Vol. 1109, pp. 303-310). (Annals of the New York Academy of Sciences; Vol. 1109). https://doi.org/10.1196/annals.1398.036

Determination of oxidized low-density lipoproteins (ox-LDL) versus ox-LDL/β2GPI complexes for the assessment of autoimmune-mediated atherosclerosis. / Lopez, Luis R.; Buckner, Tammy R.; Hurley, Beth L.; Kobayashi, Kazuko; Matsuura, Eiji.

Annals of the New York Academy of Sciences. Vol. 1109 2007. p. 303-310 (Annals of the New York Academy of Sciences; Vol. 1109).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lopez, LR, Buckner, TR, Hurley, BL, Kobayashi, K & Matsuura, E 2007, Determination of oxidized low-density lipoproteins (ox-LDL) versus ox-LDL/β2GPI complexes for the assessment of autoimmune-mediated atherosclerosis. in Annals of the New York Academy of Sciences. vol. 1109, Annals of the New York Academy of Sciences, vol. 1109, pp. 303-310. https://doi.org/10.1196/annals.1398.036
Lopez LR, Buckner TR, Hurley BL, Kobayashi K, Matsuura E. Determination of oxidized low-density lipoproteins (ox-LDL) versus ox-LDL/β2GPI complexes for the assessment of autoimmune-mediated atherosclerosis. In Annals of the New York Academy of Sciences. Vol. 1109. 2007. p. 303-310. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1398.036
Lopez, Luis R. ; Buckner, Tammy R. ; Hurley, Beth L. ; Kobayashi, Kazuko ; Matsuura, Eiji. / Determination of oxidized low-density lipoproteins (ox-LDL) versus ox-LDL/β2GPI complexes for the assessment of autoimmune-mediated atherosclerosis. Annals of the New York Academy of Sciences. Vol. 1109 2007. pp. 303-310 (Annals of the New York Academy of Sciences).
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