Determination of Hydrogen Peroxide with Phenol and 4-Aminoantipyrine by the use of a Resin Modified with Manganese-Tetrakis(sulfophenyl)porphine

Yutaka Saito; Masaki Mifune; Suzuyo Nakashima; Junichi Odo; Yoshimasa Tanaka; Masahiko Chikuma; Hisashi Tanaka

doi:10.2116/analsci.3.171

Determination of Hydrogen Peroxide with Phenol and 4-Aminoantipyrine by the use of a Resin Modified with Manganese-Tetrakis(sulfophenyl)porphine

Yutaka Saito, Masaki Mifune, Suzuyo Nakashima, Junichi Odo, Yoshimasa Tanaka, Masahiko Chikuma, Hisashi Tanaka

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

An anion-exchange resin, Amberlite IRA 900 modified with manganese-tetrakis(sulfophenyl)porphine (MnTPPS,) was used in place of peroxidase for the determination of hydrogen peroxide by the following enzyme-reaction: 2H2O2+phenol+4-aminoantipyrine peroxidase quinoid dye(λmax=505 nm)+4H2O. The calibration curve was linear in the range from 5 ng to 50 jig of hydrogen peroxide with a relative standard deviation of 1.05% (n=10). The absorbance of the quinoid dye formed by the use of Mn-TPPS, reached approximately 80% of that obtained by the control peroxidase method. The activity of MnTPPS, did not deteriorate over repeated uses. Only slight interferences of foreign substances were found except for the case of ascorbic acid.

Original language	English
Pages (from-to)	171-174
Number of pages	4
Journal	Analytical Sciences
Volume	3
Issue number	2
DOIs	https://doi.org/10.2116/analsci.3.171
Publication status	Published - 1987

Keywords

Peroxidase mimesis
hydrogen peroxide
manganese-tetrakis(sulfopheny!)porphine resin
peroxidase-like activity

ASJC Scopus subject areas

Analytical Chemistry

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10.2116/analsci.3.171

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title = "Determination of Hydrogen Peroxide with Phenol and 4-Aminoantipyrine by the use of a Resin Modified with Manganese-Tetrakis(sulfophenyl)porphine",

abstract = "An anion-exchange resin, Amberlite IRA 900 modified with manganese-tetrakis(sulfophenyl)porphine (MnTPPS,) was used in place of peroxidase for the determination of hydrogen peroxide by the following enzyme-reaction: 2H2O2+phenol+4-aminoantipyrine peroxidase quinoid dye(λmax=505 nm)+4H2O. The calibration curve was linear in the range from 5 ng to 50 jig of hydrogen peroxide with a relative standard deviation of 1.05% (n=10). The absorbance of the quinoid dye formed by the use of Mn-TPPS, reached approximately 80% of that obtained by the control peroxidase method. The activity of MnTPPS, did not deteriorate over repeated uses. Only slight interferences of foreign substances were found except for the case of ascorbic acid.",

keywords = "Peroxidase mimesis, hydrogen peroxide, manganese-tetrakis(sulfopheny!)porphine resin, peroxidase-like activity",

author = "Yutaka Saito and Masaki Mifune and Suzuyo Nakashima and Junichi Odo and Yoshimasa Tanaka and Masahiko Chikuma and Hisashi Tanaka",

year = "1987",

doi = "10.2116/analsci.3.171",

language = "English",

volume = "3",

pages = "171--174",

journal = "Analytical Sciences",

issn = "0910-6340",

publisher = "Japan Society for Analytical Chemistry",

number = "2",

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T1 - Determination of Hydrogen Peroxide with Phenol and 4-Aminoantipyrine by the use of a Resin Modified with Manganese-Tetrakis(sulfophenyl)porphine

AU - Saito, Yutaka

AU - Mifune, Masaki

AU - Nakashima, Suzuyo

AU - Odo, Junichi

AU - Tanaka, Yoshimasa

AU - Chikuma, Masahiko

AU - Tanaka, Hisashi

PY - 1987

Y1 - 1987

N2 - An anion-exchange resin, Amberlite IRA 900 modified with manganese-tetrakis(sulfophenyl)porphine (MnTPPS,) was used in place of peroxidase for the determination of hydrogen peroxide by the following enzyme-reaction: 2H2O2+phenol+4-aminoantipyrine peroxidase quinoid dye(λmax=505 nm)+4H2O. The calibration curve was linear in the range from 5 ng to 50 jig of hydrogen peroxide with a relative standard deviation of 1.05% (n=10). The absorbance of the quinoid dye formed by the use of Mn-TPPS, reached approximately 80% of that obtained by the control peroxidase method. The activity of MnTPPS, did not deteriorate over repeated uses. Only slight interferences of foreign substances were found except for the case of ascorbic acid.

AB - An anion-exchange resin, Amberlite IRA 900 modified with manganese-tetrakis(sulfophenyl)porphine (MnTPPS,) was used in place of peroxidase for the determination of hydrogen peroxide by the following enzyme-reaction: 2H2O2+phenol+4-aminoantipyrine peroxidase quinoid dye(λmax=505 nm)+4H2O. The calibration curve was linear in the range from 5 ng to 50 jig of hydrogen peroxide with a relative standard deviation of 1.05% (n=10). The absorbance of the quinoid dye formed by the use of Mn-TPPS, reached approximately 80% of that obtained by the control peroxidase method. The activity of MnTPPS, did not deteriorate over repeated uses. Only slight interferences of foreign substances were found except for the case of ascorbic acid.

KW - Peroxidase mimesis

KW - hydrogen peroxide

KW - manganese-tetrakis(sulfopheny!)porphine resin

KW - peroxidase-like activity

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DO - 10.2116/analsci.3.171

M3 - Article

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EP - 174

JO - Analytical Sciences

JF - Analytical Sciences

SN - 0910-6340

IS - 2

ER -

Determination of Hydrogen Peroxide with Phenol and 4-Aminoantipyrine by the use of a Resin Modified with Manganese-Tetrakis(sulfophenyl)porphine

Abstract

Keywords

ASJC Scopus subject areas

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