TY - JOUR
T1 - Determination of hydrogen peroxide with N,N-diethylaniline and 4-aminoantipyrine by use of an anion-exchange resin modified with manganese-tetrakis(sulphophenyl)porphine, as a substitute for peroxidase
AU - Saito, Yutaka
AU - Mifune, Masaki
AU - Nakashima, Suzuyo
AU - Odo, Junichi
AU - Tanaka, Yoshimasa
AU - Chikuma, Masahiko
AU - Tanaka, Hisashi
N1 - Funding Information:
Acknowledgement-This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan.
PY - 1987/7
Y1 - 1987/7
N2 - Amberlite IRA 900 anion-exchange resin modified with manganese-tetrakis(sulphophenyl)-porphine has been used as a catalyst instead of peroxidase for the determination of hydrogen peroxide by the reaction 2H2O2 + N,N-diethylaniline + 4-aminoantipyrine catalyst→ quinonoid dye (λmax 550 nm) + 4H2O. The apparent molar absorptivity for hydrogen peroxide was 1.1 × 104 1.mole-1.cm-1, coefficient of variation 0.7%. This value is approximately 84% of that obtained by the use of peroxidase as catalyst. Similar conditions to those in the enzymatic reaction were suitable for use of the modified resin as catalyst, and the results show it to be a good substitute for peroxidase in this reaction system.
AB - Amberlite IRA 900 anion-exchange resin modified with manganese-tetrakis(sulphophenyl)-porphine has been used as a catalyst instead of peroxidase for the determination of hydrogen peroxide by the reaction 2H2O2 + N,N-diethylaniline + 4-aminoantipyrine catalyst→ quinonoid dye (λmax 550 nm) + 4H2O. The apparent molar absorptivity for hydrogen peroxide was 1.1 × 104 1.mole-1.cm-1, coefficient of variation 0.7%. This value is approximately 84% of that obtained by the use of peroxidase as catalyst. Similar conditions to those in the enzymatic reaction were suitable for use of the modified resin as catalyst, and the results show it to be a good substitute for peroxidase in this reaction system.
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U2 - 10.1016/0039-9140(87)80086-3
DO - 10.1016/0039-9140(87)80086-3
M3 - Article
AN - SCOPUS:0000187085
VL - 34
SP - 667
EP - 669
JO - Talanta
JF - Talanta
SN - 0039-9140
IS - 7
ER -