Determination of hydrogen peroxide with N,N-diethylaniline and 4-aminoantipyrine by use of an anion-exchange resin modified with manganese-tetrakis(sulphophenyl)porphine, as a substitute for peroxidase

Yutaka Saito, Masaki Mifune, Suzuyo Nakashima, Junichi Odo, Yoshimasa Tanaka, Masahiko Chikuma, Hisashi Tanaka

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Abstract

Amberlite IRA 900 anion-exchange resin modified with manganese-tetrakis(sulphophenyl)-porphine has been used as a catalyst instead of peroxidase for the determination of hydrogen peroxide by the reaction 2H2O2 + N,N-diethylaniline + 4-aminoantipyrine catalyst→ quinonoid dye (λmax 550 nm) + 4H2O. The apparent molar absorptivity for hydrogen peroxide was 1.1 × 104 1.mole-1.cm-1, coefficient of variation 0.7%. This value is approximately 84% of that obtained by the use of peroxidase as catalyst. Similar conditions to those in the enzymatic reaction were suitable for use of the modified resin as catalyst, and the results show it to be a good substitute for peroxidase in this reaction system.

Original languageEnglish
Pages (from-to)667-669
Number of pages3
JournalTalanta
Volume34
Issue number7
DOIs
Publication statusPublished - Jul 1987

ASJC Scopus subject areas

  • Analytical Chemistry

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