Determination of histone by membrane filter collection with 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone and manganese (II)

Hiroshi Tominaga, Hiroko Kadobayashi, Takako Yamaguchi, Shinichiro Kamino, Michiko Nishimura, Yoshikazu Fujita

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We reported on a spectrophotometric determination of DNA binding protein, histone, with 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone and manganese(II) in a previous paper. In the present work, preconcentration method for the determination of histone, which involves the filtration of a histone - 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone - manganese (II) aggregate on a membrane filter, followed by its redissolution or tristimulus colorimetry, is described. Each of these methods is about 10-times more sensitive than the usual spectrophotometry.

Original languageEnglish
Pages (from-to)127-131
Number of pages5
JournalBunseki Kagaku
Volume55
Issue number2
DOIs
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

Manganese
Histones
Membranes
Colorimetry
DNA-Binding Proteins
Spectrophotometry

Keywords

  • 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone
  • Histone determination
  • Manganese(II)
  • Membrane filter

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Determination of histone by membrane filter collection with 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone and manganese (II). / Tominaga, Hiroshi; Kadobayashi, Hiroko; Yamaguchi, Takako; Kamino, Shinichiro; Nishimura, Michiko; Fujita, Yoshikazu.

In: Bunseki Kagaku, Vol. 55, No. 2, 2006, p. 127-131.

Research output: Contribution to journalArticle

Tominaga, H, Kadobayashi, H, Yamaguchi, T, Kamino, S, Nishimura, M & Fujita, Y 2006, 'Determination of histone by membrane filter collection with 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone and manganese (II)', Bunseki Kagaku, vol. 55, no. 2, pp. 127-131. https://doi.org/10.2116/bunsekikagaku.55.127
Tominaga, Hiroshi ; Kadobayashi, Hiroko ; Yamaguchi, Takako ; Kamino, Shinichiro ; Nishimura, Michiko ; Fujita, Yoshikazu. / Determination of histone by membrane filter collection with 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone and manganese (II). In: Bunseki Kagaku. 2006 ; Vol. 55, No. 2. pp. 127-131.
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