Determination of histone by membrane filter collection with 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone and manganese (II)

Hiroshi Tominaga, Hiroko Kadobayashi, Takako Yamaguchi, Shinichiro Kamino, Michiko Nishimura, Yoshikazu Fujita

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We reported on a spectrophotometric determination of DNA binding protein, histone, with 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone and manganese(II) in a previous paper. In the present work, preconcentration method for the determination of histone, which involves the filtration of a histone - 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone - manganese (II) aggregate on a membrane filter, followed by its redissolution or tristimulus colorimetry, is described. Each of these methods is about 10-times more sensitive than the usual spectrophotometry.

Original languageEnglish
Pages (from-to)127-131
Number of pages5
JournalBunseki Kagaku
Volume55
Issue number2
DOIs
Publication statusPublished - 2006
Externally publishedYes

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Keywords

  • 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone
  • Histone determination
  • Manganese(II)
  • Membrane filter

ASJC Scopus subject areas

  • Analytical Chemistry

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