Determination of growth stages and metabolic profiles in Brachypodium distachyon for comparison of developmental context with Triticeae crops

Yoshihiko Onda, Kei Hashimoto, Takuhiro Yoshida, Tetsuya Sakurai, Yuji Sawada, Masami Yokota Hirai, Kiminori Toyooka, Keiichi Mochida, Kazuo Shinozaki

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Brachypodium distachyon is an emerging model plant for studying biological phenomena in temperate grasses. Study of the growth scale is essential to analyse spatio-temporal changes in molecular factors throughout the life cycle. For sensitive and robust staging based on morphology in B. distachyon, we demonstrated the utility of the BBCH (Biologische Bundesanstalt, Bundessortenamt and CHemical industry) scale, which is comparable to the Zadoks scale conventionally used for Triticeae crops. We compared the chronological progression of B. distachyon accessions Bd21 and Bd3-1, in addition to the progression of Chinese Spring wheat. The comparison of growth stages illustrates the morphological similarities and differences in the timing of life cycle events. Furthermore,we compared metabolite accumulation patterns across different growth stages and across different stress conditions using a widely targeted metabolome analysis. Metabolic profiling determined commonalities and specificities in chemical properties that were dependent on organisms, growth stages and/or stress conditions. Most metabolites accumulated equivalently in B. distachyon and wheat. This qualitative similarity indicated the superiority of B. distachyon as a model for Triticeae crops. The growth scale of B. distachyon should provide a conceptual framework for comparative analysis and for knowledge integration between this model grass and crops in the Pooideae subfamily.

Original languageEnglish
Article number20150964
JournalProceedings of the Royal Society B: Biological Sciences
Volume282
Issue number1811
DOIs
Publication statusPublished - Jul 8 2015
Externally publishedYes

Fingerprint

Brachypodium
Brachypodium distachyon
Metabolome
Crops
developmental stages
crop
crops
Growth
Metabolites
metabolomics
Life cycle
Poaceae
Life Cycle Stages
metabolite
life cycle
wheat
Triticum
grass
life cycle (organisms)
Spatio-Temporal Analysis

Keywords

  • BBCH scale
  • Brachypodium distachyon
  • Growth staging
  • Metabolome
  • Pooideae
  • Zadoks scale

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Determination of growth stages and metabolic profiles in Brachypodium distachyon for comparison of developmental context with Triticeae crops. / Onda, Yoshihiko; Hashimoto, Kei; Yoshida, Takuhiro; Sakurai, Tetsuya; Sawada, Yuji; Hirai, Masami Yokota; Toyooka, Kiminori; Mochida, Keiichi; Shinozaki, Kazuo.

In: Proceedings of the Royal Society B: Biological Sciences, Vol. 282, No. 1811, 20150964, 08.07.2015.

Research output: Contribution to journalArticle

Onda, Yoshihiko ; Hashimoto, Kei ; Yoshida, Takuhiro ; Sakurai, Tetsuya ; Sawada, Yuji ; Hirai, Masami Yokota ; Toyooka, Kiminori ; Mochida, Keiichi ; Shinozaki, Kazuo. / Determination of growth stages and metabolic profiles in Brachypodium distachyon for comparison of developmental context with Triticeae crops. In: Proceedings of the Royal Society B: Biological Sciences. 2015 ; Vol. 282, No. 1811.
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