Determination of formaldehyde in urine by headspace gas chromatography

Akito Takeuchi; Tomoko Takigawa; Mikiko Abe; Toshio Kawai; Yoko Endo; Tomojiro Yasugi; Ginji Endo; Keiki Ogino

doi:10.1007/s00128-007-9172-0

Determination of formaldehyde in urine by headspace gas chromatography

Akito Takeuchi, Tomoko Takigawa, Mikiko Abe, Toshio Kawai, Yoko Endo, Tomojiro Yasugi, Ginji Endo, Keiki Ogino

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

Formaldehyde is a carcinogen to which humans are exposed daily, but few methods are available to quantify formaldehyde in biological samples. We developed a simple, sensitive and rapid technique for the quantification of formaldehyde in urine by derivatization with O-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine, using a headspace sampler coupled to a gas chromatograph equipped with an electron capture detector. The detection limit was 1.08 μg/L. The overall recovery of formaldehyde spiked in urine was 99%. The concentration of formaldehyde in urine obtained from healthy volunteers ranged from 56.85 to 144.57 μg/L. This method can be used successfully to measure formaldehyde in urine.

Original language	English
Pages (from-to)	1-4
Number of pages	4
Journal	Bulletin of Environmental Contamination and Toxicology
Volume	79
Issue number	1
DOIs	https://doi.org/10.1007/s00128-007-9172-0
Publication status	Published - Jul 2007

Keywords

Formaldehyde
Gas chromatography
Headspace analysis
Human urine
O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine

ASJC Scopus subject areas

Toxicology
Pollution
Health, Toxicology and Mutagenesis

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abstract = "Formaldehyde is a carcinogen to which humans are exposed daily, but few methods are available to quantify formaldehyde in biological samples. We developed a simple, sensitive and rapid technique for the quantification of formaldehyde in urine by derivatization with O-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine, using a headspace sampler coupled to a gas chromatograph equipped with an electron capture detector. The detection limit was 1.08 μg/L. The overall recovery of formaldehyde spiked in urine was 99%. The concentration of formaldehyde in urine obtained from healthy volunteers ranged from 56.85 to 144.57 μg/L. This method can be used successfully to measure formaldehyde in urine.",

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AU - Takeuchi, Akito

AU - Takigawa, Tomoko

AU - Abe, Mikiko

AU - Kawai, Toshio

AU - Endo, Yoko

AU - Yasugi, Tomojiro

AU - Endo, Ginji

AU - Ogino, Keiki

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