Determination of formaldehyde in urine by headspace gas chromatography

Akito Takeuchi, Tomoko Takigawa, Mikiko Abe, Toshio Kawai, Yoko Endo, Tomojiro Yasugi, Ginji Endo, Keiki Ogino

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Formaldehyde is a carcinogen to which humans are exposed daily, but few methods are available to quantify formaldehyde in biological samples. We developed a simple, sensitive and rapid technique for the quantification of formaldehyde in urine by derivatization with O-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine, using a headspace sampler coupled to a gas chromatograph equipped with an electron capture detector. The detection limit was 1.08 μg/L. The overall recovery of formaldehyde spiked in urine was 99%. The concentration of formaldehyde in urine obtained from healthy volunteers ranged from 56.85 to 144.57 μg/L. This method can be used successfully to measure formaldehyde in urine.

Original languageEnglish
Pages (from-to)1-4
Number of pages4
JournalBulletin of Environmental Contamination and Toxicology
Volume79
Issue number1
DOIs
Publication statusPublished - Jul 2007

Fingerprint

formaldehyde
Formaldehyde
Gas chromatography
Gas Chromatography
urine
gas chromatography
Urine
Carcinogens
Hydroxylamine
carcinogen
sampler
Limit of Detection
Healthy Volunteers
Gases
Electrons
Detectors
Recovery
electron
gas

Keywords

  • Formaldehyde
  • Gas chromatography
  • Headspace analysis
  • Human urine
  • O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Toxicology

Cite this

Determination of formaldehyde in urine by headspace gas chromatography. / Takeuchi, Akito; Takigawa, Tomoko; Abe, Mikiko; Kawai, Toshio; Endo, Yoko; Yasugi, Tomojiro; Endo, Ginji; Ogino, Keiki.

In: Bulletin of Environmental Contamination and Toxicology, Vol. 79, No. 1, 07.2007, p. 1-4.

Research output: Contribution to journalArticle

Takeuchi, A, Takigawa, T, Abe, M, Kawai, T, Endo, Y, Yasugi, T, Endo, G & Ogino, K 2007, 'Determination of formaldehyde in urine by headspace gas chromatography', Bulletin of Environmental Contamination and Toxicology, vol. 79, no. 1, pp. 1-4. https://doi.org/10.1007/s00128-007-9172-0
Takeuchi, Akito ; Takigawa, Tomoko ; Abe, Mikiko ; Kawai, Toshio ; Endo, Yoko ; Yasugi, Tomojiro ; Endo, Ginji ; Ogino, Keiki. / Determination of formaldehyde in urine by headspace gas chromatography. In: Bulletin of Environmental Contamination and Toxicology. 2007 ; Vol. 79, No. 1. pp. 1-4.
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