Determination of formaldehyde in urine by headspace gas chromatography

Akito Takeuchi, Tomoko Takigawa, Mikiko Abe, Toshio Kawai, Yoko Endo, Tomojiro Yasugi, Ginji Endo, Keiki Ogino

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)


Formaldehyde is a carcinogen to which humans are exposed daily, but few methods are available to quantify formaldehyde in biological samples. We developed a simple, sensitive and rapid technique for the quantification of formaldehyde in urine by derivatization with O-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine, using a headspace sampler coupled to a gas chromatograph equipped with an electron capture detector. The detection limit was 1.08 μg/L. The overall recovery of formaldehyde spiked in urine was 99%. The concentration of formaldehyde in urine obtained from healthy volunteers ranged from 56.85 to 144.57 μg/L. This method can be used successfully to measure formaldehyde in urine.

Original languageEnglish
Pages (from-to)1-4
Number of pages4
JournalBulletin of Environmental Contamination and Toxicology
Issue number1
Publication statusPublished - Jul 2007


  • Formaldehyde
  • Gas chromatography
  • Headspace analysis
  • Human urine
  • O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine

ASJC Scopus subject areas

  • Toxicology
  • Pollution
  • Health, Toxicology and Mutagenesis


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