Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL

Kunio Hirata; Kyoko Shinzawa-Itoh; Naomine Yano; Shuhei Takemura; Koji Kato; Miki Hatanaka; Kazumasa Muramoto; Takako Kawahara; Tomitake Tsukihara; Eiki Yamashita; Kensuke Tono; Go Ueno; Takaaki Hikima; Hironori Murakami; Yuichi Inubushi; Makina Yabashi; Tetsuya Ishikawa; Masaki Yamamoto; Takashi Ogura; Hiroshi Sugimoto; Jian Ren Shen; Shinya Yoshikawa; Hideo Ago

doi:10.1038/nmeth.2962

Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL

Kunio Hirata, Kyoko Shinzawa-Itoh, Naomine Yano, Shuhei Takemura, Koji Kato, Miki Hatanaka, Kazumasa Muramoto, Takako Kawahara, Tomitake Tsukihara, Eiki Yamashita, Kensuke Tono, Go Ueno, Takaaki Hikima, Hironori Murakami, Yuichi Inubushi, Makina Yabashi, Tetsuya Ishikawa, Masaki Yamamoto, Takashi Ogura, Hiroshi SugimotoJian Ren Shen, Shinya Yoshikawa, Hideo Ago

Research Institute for Interdisciplinary Science

Research output: Contribution to journal › Article › peer-review

176 Citations (Scopus)

Abstract

We report a method of femtosecond crystallography for solving radiation damage-free crystal structures of large proteins at sub-angstrom spatial resolution, using a large single crystal and the femtosecond pulses of an X-ray free-electron laser (XFEL). We demonstrated the performance of the method by determining a 1.9-Å radiation damage-free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.

Original language	English
Pages (from-to)	734-736
Number of pages	3
Journal	Nature Methods
Volume	11
Issue number	7
DOIs	https://doi.org/10.1038/nmeth.2962
Publication status	Published - Jul 2014

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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Hirata, K., Shinzawa-Itoh, K., Yano, N., Takemura, S., Kato, K., Hatanaka, M., Muramoto, K., Kawahara, T., Tsukihara, T., Yamashita, E., Tono, K., Ueno, G., Hikima, T., Murakami, H., Inubushi, Y., Yabashi, M., Ishikawa, T., Yamamoto, M., Ogura, T., ... Ago, H. (2014). Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL. Nature Methods, 11(7), 734-736. https://doi.org/10.1038/nmeth.2962

Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL. / Hirata, Kunio; Shinzawa-Itoh, Kyoko; Yano, Naomine; Takemura, Shuhei; Kato, Koji; Hatanaka, Miki; Muramoto, Kazumasa; Kawahara, Takako; Tsukihara, Tomitake; Yamashita, Eiki; Tono, Kensuke; Ueno, Go; Hikima, Takaaki; Murakami, Hironori; Inubushi, Yuichi; Yabashi, Makina; Ishikawa, Tetsuya; Yamamoto, Masaki; Ogura, Takashi; Sugimoto, Hiroshi; Shen, Jian Ren; Yoshikawa, Shinya; Ago, Hideo.

In: Nature Methods, Vol. 11, No. 7, 07.2014, p. 734-736.

Research output: Contribution to journal › Article › peer-review

Hirata, K, Shinzawa-Itoh, K, Yano, N, Takemura, S, Kato, K, Hatanaka, M, Muramoto, K, Kawahara, T, Tsukihara, T, Yamashita, E, Tono, K, Ueno, G, Hikima, T, Murakami, H, Inubushi, Y, Yabashi, M, Ishikawa, T, Yamamoto, M, Ogura, T, Sugimoto, H, Shen, JR, Yoshikawa, S & Ago, H 2014, 'Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL', Nature Methods, vol. 11, no. 7, pp. 734-736. https://doi.org/10.1038/nmeth.2962

Hirata, Kunio ; Shinzawa-Itoh, Kyoko ; Yano, Naomine ; Takemura, Shuhei ; Kato, Koji ; Hatanaka, Miki ; Muramoto, Kazumasa ; Kawahara, Takako ; Tsukihara, Tomitake ; Yamashita, Eiki ; Tono, Kensuke ; Ueno, Go ; Hikima, Takaaki ; Murakami, Hironori ; Inubushi, Yuichi ; Yabashi, Makina ; Ishikawa, Tetsuya ; Yamamoto, Masaki ; Ogura, Takashi ; Sugimoto, Hiroshi ; Shen, Jian Ren ; Yoshikawa, Shinya ; Ago, Hideo. / Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL. In: Nature Methods. 2014 ; Vol. 11, No. 7. pp. 734-736.

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abstract = "We report a method of femtosecond crystallography for solving radiation damage-free crystal structures of large proteins at sub-angstrom spatial resolution, using a large single crystal and the femtosecond pulses of an X-ray free-electron laser (XFEL). We demonstrated the performance of the method by determining a 1.9-{\AA} radiation damage-free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.",

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AU - Kato, Koji

AU - Hatanaka, Miki

AU - Muramoto, Kazumasa

AU - Kawahara, Takako

AU - Tsukihara, Tomitake

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AU - Inubushi, Yuichi

AU - Yabashi, Makina

AU - Ishikawa, Tetsuya

AU - Yamamoto, Masaki

AU - Ogura, Takashi

AU - Sugimoto, Hiroshi

AU - Shen, Jian Ren

AU - Yoshikawa, Shinya

AU - Ago, Hideo

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Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL

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