Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL

Kunio Hirata; Kyoko Shinzawa-Itoh; Naomine Yano; Shuhei Takemura; Koji Kato; Miki Hatanaka; Kazumasa Muramoto; Takako Kawahara; Tomitake Tsukihara; Eiki Yamashita; Kensuke Tono; Go Ueno; Takaaki Hikima; Hironori Murakami; Yuichi Inubushi; Makina Yabashi; Tetsuya Ishikawa; Masaki Yamamoto; Takashi Ogura; Hiroshi Sugimoto; Jian Ren Shen; Shinya Yoshikawa; Hideo Ago

doi:10.1038/nmeth.2962

Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL

Kunio Hirata, Kyoko Shinzawa-Itoh, Naomine Yano, Shuhei Takemura, Koji Kato, Miki Hatanaka, Kazumasa Muramoto, Takako Kawahara, Tomitake Tsukihara, Eiki Yamashita, Kensuke Tono, Go Ueno, Takaaki Hikima, Hironori Murakami, Yuichi Inubushi, Makina Yabashi, Tetsuya Ishikawa, Masaki Yamamoto, Takashi Ogura, Hiroshi SugimotoJian Ren Shen, Shinya Yoshikawa, Hideo Ago

Research Institute for Interdisciplinary Science

Research output: Contribution to journal › Article › peer-review

201 Citations (Scopus)

Abstract

We report a method of femtosecond crystallography for solving radiation damage-free crystal structures of large proteins at sub-angstrom spatial resolution, using a large single crystal and the femtosecond pulses of an X-ray free-electron laser (XFEL). We demonstrated the performance of the method by determining a 1.9-Å radiation damage-free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.

Original language	English
Pages (from-to)	734-736
Number of pages	3
Journal	Nature Methods
Volume	11
Issue number	7
DOIs	https://doi.org/10.1038/nmeth.2962
Publication status	Published - Jul 2014

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1038/nmeth.2962

Cite this

Hirata, K., Shinzawa-Itoh, K., Yano, N., Takemura, S., Kato, K., Hatanaka, M., Muramoto, K., Kawahara, T., Tsukihara, T., Yamashita, E., Tono, K., Ueno, G., Hikima, T., Murakami, H., Inubushi, Y., Yabashi, M., Ishikawa, T., Yamamoto, M., Ogura, T., ... Ago, H. (2014). Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL. Nature Methods, 11(7), 734-736. https://doi.org/10.1038/nmeth.2962

Hirata, K, Shinzawa-Itoh, K, Yano, N, Takemura, S, Kato, K, Hatanaka, M, Muramoto, K, Kawahara, T, Tsukihara, T, Yamashita, E, Tono, K, Ueno, G, Hikima, T, Murakami, H, Inubushi, Y, Yabashi, M, Ishikawa, T, Yamamoto, M, Ogura, T, Sugimoto, H, Shen, JR, Yoshikawa, S & Ago, H 2014, 'Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL', Nature Methods, vol. 11, no. 7, pp. 734-736. https://doi.org/10.1038/nmeth.2962

@article{006aae1c0744411e9ac1c237b9da662b,

title = "Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL",

abstract = "We report a method of femtosecond crystallography for solving radiation damage-free crystal structures of large proteins at sub-angstrom spatial resolution, using a large single crystal and the femtosecond pulses of an X-ray free-electron laser (XFEL). We demonstrated the performance of the method by determining a 1.9-{\AA} radiation damage-free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.",

author = "Kunio Hirata and Kyoko Shinzawa-Itoh and Naomine Yano and Shuhei Takemura and Koji Kato and Miki Hatanaka and Kazumasa Muramoto and Takako Kawahara and Tomitake Tsukihara and Eiki Yamashita and Kensuke Tono and Go Ueno and Takaaki Hikima and Hironori Murakami and Yuichi Inubushi and Makina Yabashi and Tetsuya Ishikawa and Masaki Yamamoto and Takashi Ogura and Hiroshi Sugimoto and Shen, {Jian Ren} and Shinya Yoshikawa and Hideo Ago",

year = "2014",

month = jul,

doi = "10.1038/nmeth.2962",

language = "English",

volume = "11",

pages = "734--736",

journal = "PLoS Medicine",

issn = "1549-1277",

publisher = "Public Library of Science",

number = "7",

}

TY - JOUR

T1 - Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL

AU - Hirata, Kunio

AU - Shinzawa-Itoh, Kyoko

AU - Yano, Naomine

AU - Takemura, Shuhei

AU - Kato, Koji

AU - Hatanaka, Miki

AU - Muramoto, Kazumasa

AU - Kawahara, Takako

AU - Tsukihara, Tomitake

AU - Yamashita, Eiki

AU - Tono, Kensuke

AU - Ueno, Go

AU - Hikima, Takaaki

AU - Murakami, Hironori

AU - Inubushi, Yuichi

AU - Yabashi, Makina

AU - Ishikawa, Tetsuya

AU - Yamamoto, Masaki

AU - Ogura, Takashi

AU - Sugimoto, Hiroshi

AU - Shen, Jian Ren

AU - Yoshikawa, Shinya

AU - Ago, Hideo

PY - 2014/7

Y1 - 2014/7

N2 - We report a method of femtosecond crystallography for solving radiation damage-free crystal structures of large proteins at sub-angstrom spatial resolution, using a large single crystal and the femtosecond pulses of an X-ray free-electron laser (XFEL). We demonstrated the performance of the method by determining a 1.9-Å radiation damage-free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.

AB - We report a method of femtosecond crystallography for solving radiation damage-free crystal structures of large proteins at sub-angstrom spatial resolution, using a large single crystal and the femtosecond pulses of an X-ray free-electron laser (XFEL). We demonstrated the performance of the method by determining a 1.9-Å radiation damage-free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.

UR - http://www.scopus.com/inward/record.url?scp=84903612491&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903612491&partnerID=8YFLogxK

U2 - 10.1038/nmeth.2962

DO - 10.1038/nmeth.2962

M3 - Article

C2 - 24813624

AN - SCOPUS:84903612491

VL - 11

SP - 734

EP - 736

JO - PLoS Medicine

JF - PLoS Medicine

SN - 1549-1277

IS - 7

ER -

Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this