Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL

Kunio Hirata, Kyoko Shinzawa-Itoh, Naomine Yano, Shuhei Takemura, Koji Kato, Miki Hatanaka, Kazumasa Muramoto, Takako Kawahara, Tomitake Tsukihara, Eiki Yamashita, Kensuke Tono, Go Ueno, Takaaki Hikima, Hironori Murakami, Yuichi Inubushi, Makina Yabashi, Tetsuya Ishikawa, Masaki Yamamoto, Takashi Ogura, Hiroshi SugimotoJian Ren Shen, Shinya Yoshikawa, Hideo Ago

Research output: Contribution to journalArticle

166 Citations (Scopus)

Abstract

We report a method of femtosecond crystallography for solving radiation damage-free crystal structures of large proteins at sub-angstrom spatial resolution, using a large single crystal and the femtosecond pulses of an X-ray free-electron laser (XFEL). We demonstrated the performance of the method by determining a 1.9-Å radiation damage-free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.

Original languageEnglish
Pages (from-to)734-736
Number of pages3
JournalNature Methods
Volume11
Issue number7
DOIs
Publication statusPublished - Jul 2014

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Hirata, K., Shinzawa-Itoh, K., Yano, N., Takemura, S., Kato, K., Hatanaka, M., Muramoto, K., Kawahara, T., Tsukihara, T., Yamashita, E., Tono, K., Ueno, G., Hikima, T., Murakami, H., Inubushi, Y., Yabashi, M., Ishikawa, T., Yamamoto, M., Ogura, T., ... Ago, H. (2014). Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL. Nature Methods, 11(7), 734-736. https://doi.org/10.1038/nmeth.2962