Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL

Kunio Hirata, Kyoko Shinzawa-Itoh, Naomine Yano, Shuhei Takemura, Koji Kato, Miki Hatanaka, Kazumasa Muramoto, Takako Kawahara, Tomitake Tsukihara, Eiki Yamashita, Kensuke Tono, Go Ueno, Takaaki Hikima, Hironori Murakami, Yuichi Inubushi, Makina Yabashi, Tetsuya Ishikawa, Masaki Yamamoto, Takashi Ogura, Hiroshi SugimotoJian-Ren Shen, Shinya Yoshikawa, Hideo Ago

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

We report a method of femtosecond crystallography for solving radiation damage-free crystal structures of large proteins at sub-angstrom spatial resolution, using a large single crystal and the femtosecond pulses of an X-ray free-electron laser (XFEL). We demonstrated the performance of the method by determining a 1.9-Å radiation damage-free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.

Original languageEnglish
Pages (from-to)734-736
Number of pages3
JournalNature Methods
Volume11
Issue number7
DOIs
Publication statusPublished - 2014

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X ray lasers
Free electron lasers
Radiation damage
Lasers
Crystal structure
X-Rays
Electrons
Radiation
X rays
Crystallography
Electron Transport Complex IV
Ultrashort pulses
Membrane Proteins
Proteins
Single crystals

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Hirata, K., Shinzawa-Itoh, K., Yano, N., Takemura, S., Kato, K., Hatanaka, M., ... Ago, H. (2014). Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL. Nature Methods, 11(7), 734-736. https://doi.org/10.1038/nmeth.2962

Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL. / Hirata, Kunio; Shinzawa-Itoh, Kyoko; Yano, Naomine; Takemura, Shuhei; Kato, Koji; Hatanaka, Miki; Muramoto, Kazumasa; Kawahara, Takako; Tsukihara, Tomitake; Yamashita, Eiki; Tono, Kensuke; Ueno, Go; Hikima, Takaaki; Murakami, Hironori; Inubushi, Yuichi; Yabashi, Makina; Ishikawa, Tetsuya; Yamamoto, Masaki; Ogura, Takashi; Sugimoto, Hiroshi; Shen, Jian-Ren; Yoshikawa, Shinya; Ago, Hideo.

In: Nature Methods, Vol. 11, No. 7, 2014, p. 734-736.

Research output: Contribution to journalArticle

Hirata, K, Shinzawa-Itoh, K, Yano, N, Takemura, S, Kato, K, Hatanaka, M, Muramoto, K, Kawahara, T, Tsukihara, T, Yamashita, E, Tono, K, Ueno, G, Hikima, T, Murakami, H, Inubushi, Y, Yabashi, M, Ishikawa, T, Yamamoto, M, Ogura, T, Sugimoto, H, Shen, J-R, Yoshikawa, S & Ago, H 2014, 'Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL', Nature Methods, vol. 11, no. 7, pp. 734-736. https://doi.org/10.1038/nmeth.2962
Hirata, Kunio ; Shinzawa-Itoh, Kyoko ; Yano, Naomine ; Takemura, Shuhei ; Kato, Koji ; Hatanaka, Miki ; Muramoto, Kazumasa ; Kawahara, Takako ; Tsukihara, Tomitake ; Yamashita, Eiki ; Tono, Kensuke ; Ueno, Go ; Hikima, Takaaki ; Murakami, Hironori ; Inubushi, Yuichi ; Yabashi, Makina ; Ishikawa, Tetsuya ; Yamamoto, Masaki ; Ogura, Takashi ; Sugimoto, Hiroshi ; Shen, Jian-Ren ; Yoshikawa, Shinya ; Ago, Hideo. / Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL. In: Nature Methods. 2014 ; Vol. 11, No. 7. pp. 734-736.
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