Determination of cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid in the urine of a patient with cystathioninuria using column liquid chromatography-mass spectrometry

Kazunori Sugahara, Jun Ohta, Megumi Takemura, Hiroyuki Kodama

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A method for the measurement of cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid in the urine of a patient with cystathioninuria has been developed, using column liquid chromatography-mass spectrometry. Cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid were determined by scanning the [M + H]+ ions of each compound. The recoveries were 80-92.4% for cystathionine and 80-100% for perhydro-1,4-thiazepine-3,5-dicarboxylic acid after ion-exchange treatment. The results agreed well with those obtained using an amino acid analyser. The concentrations found for cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid were 1.289 ± 0.099 mg/ml and 0.310 ± 0.0067 mg/ml, respectively.

Original languageEnglish
Pages (from-to)318-323
Number of pages6
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume579
Issue number2
DOIs
Publication statusPublished - Sep 4 1992

ASJC Scopus subject areas

  • Chemistry(all)

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