Determination of cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid in the urine of a patient with cystathioninuria using column liquid chromatography-mass spectrometry

Kazunori Sugahara, Jun Ohta, Megumi Takemura, Hiroyuki Kodama

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A method for the measurement of cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid in the urine of a patient with cystathioninuria has been developed, using column liquid chromatography-mass spectrometry. Cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid were determined by scanning the [M + H]+ ions of each compound. The recoveries were 80-92.4% for cystathionine and 80-100% for perhydro-1,4-thiazepine-3,5-dicarboxylic acid after ion-exchange treatment. The results agreed well with those obtained using an amino acid analyser. The concentrations found for cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid were 1.289 ± 0.099 mg/ml and 0.310 ± 0.0067 mg/ml, respectively.

Original languageEnglish
Pages (from-to)318-323
Number of pages6
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume579
Issue number2
DOIs
Publication statusPublished - Sep 4 1992

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Cystathionine
Column chromatography
Liquid chromatography
Liquid Chromatography
Mass spectrometry
Mass Spectrometry
Urine
Ion Exchange
Ion exchange
Ions
Scanning
Amino Acids
Recovery
perhydro-1,4-thiazepine-3,5-dicarboxylic acid
Gamma-cystathionase deficiency

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

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title = "Determination of cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid in the urine of a patient with cystathioninuria using column liquid chromatography-mass spectrometry",
abstract = "A method for the measurement of cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid in the urine of a patient with cystathioninuria has been developed, using column liquid chromatography-mass spectrometry. Cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid were determined by scanning the [M + H]+ ions of each compound. The recoveries were 80-92.4{\%} for cystathionine and 80-100{\%} for perhydro-1,4-thiazepine-3,5-dicarboxylic acid after ion-exchange treatment. The results agreed well with those obtained using an amino acid analyser. The concentrations found for cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid were 1.289 ± 0.099 mg/ml and 0.310 ± 0.0067 mg/ml, respectively.",
author = "Kazunori Sugahara and Jun Ohta and Megumi Takemura and Hiroyuki Kodama",
year = "1992",
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doi = "10.1016/0378-4347(92)80397-9",
language = "English",
volume = "579",
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T1 - Determination of cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid in the urine of a patient with cystathioninuria using column liquid chromatography-mass spectrometry

AU - Sugahara, Kazunori

AU - Ohta, Jun

AU - Takemura, Megumi

AU - Kodama, Hiroyuki

PY - 1992/9/4

Y1 - 1992/9/4

N2 - A method for the measurement of cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid in the urine of a patient with cystathioninuria has been developed, using column liquid chromatography-mass spectrometry. Cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid were determined by scanning the [M + H]+ ions of each compound. The recoveries were 80-92.4% for cystathionine and 80-100% for perhydro-1,4-thiazepine-3,5-dicarboxylic acid after ion-exchange treatment. The results agreed well with those obtained using an amino acid analyser. The concentrations found for cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid were 1.289 ± 0.099 mg/ml and 0.310 ± 0.0067 mg/ml, respectively.

AB - A method for the measurement of cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid in the urine of a patient with cystathioninuria has been developed, using column liquid chromatography-mass spectrometry. Cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid were determined by scanning the [M + H]+ ions of each compound. The recoveries were 80-92.4% for cystathionine and 80-100% for perhydro-1,4-thiazepine-3,5-dicarboxylic acid after ion-exchange treatment. The results agreed well with those obtained using an amino acid analyser. The concentrations found for cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid were 1.289 ± 0.099 mg/ml and 0.310 ± 0.0067 mg/ml, respectively.

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