TY - JOUR
T1 - Determination of cyanine-labeled amino acid enantiomers by cyclodextrin- modified capillary gel electrophoresis combined with diode laser fluorescence detection
AU - Kaneta, Takashi
AU - Shiba, Hiroki
AU - Imasaka, Totaro
N1 - Funding Information:
This research is supported by Grants-in-Aid for Scientific Research from the Ministry of Education of Japan. T.K. thanks Mr. Kenneth P. Roberts for helpful discussions.
PY - 1998/5/1
Y1 - 1998/5/1
N2 - Enantiomeric amino acids were labeled with a cyanine derivative (Cy5) for selective determination by capillary electrophoresis combined with diode laser-induced fluorometry. Cyanine-labeled glutamic acid has a high negative charge so that it was difficult to detect by conventional capillary zone electrophoresis due to its electrophoretic mobility being greater than the electroosmotic mobility. Thus, cyclodextrin-modified capillary gel electrophoresis using replaceable poly(vinyl-pyrrolidone) (PVP), which effectively suppresses the electroosmotic flow, was employed to separate Cy5- labeled amino acid enantiomers. The separation of six amino acid enantiomers could be achieved by using 1% PVP solution containing 70 mM γ-cyclodextrin. It was also shown that PVP does not affect the selectivity of optical isomers.
AB - Enantiomeric amino acids were labeled with a cyanine derivative (Cy5) for selective determination by capillary electrophoresis combined with diode laser-induced fluorometry. Cyanine-labeled glutamic acid has a high negative charge so that it was difficult to detect by conventional capillary zone electrophoresis due to its electrophoretic mobility being greater than the electroosmotic mobility. Thus, cyclodextrin-modified capillary gel electrophoresis using replaceable poly(vinyl-pyrrolidone) (PVP), which effectively suppresses the electroosmotic flow, was employed to separate Cy5- labeled amino acid enantiomers. The separation of six amino acid enantiomers could be achieved by using 1% PVP solution containing 70 mM γ-cyclodextrin. It was also shown that PVP does not affect the selectivity of optical isomers.
KW - Amino acids
KW - Cyanine labelling
KW - Derivatization, electrophoresis
KW - Enantiomer separation
KW - Poly(vinylpyrrolidone)
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U2 - 10.1016/S0021-9673(98)00023-5
DO - 10.1016/S0021-9673(98)00023-5
M3 - Article
AN - SCOPUS:0032076470
VL - 805
SP - 295
EP - 300
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 1-2
ER -