Determination of cyanine-labeled amino acid enantiomers by cyclodextrin- modified capillary gel electrophoresis combined with diode laser fluorescence detection

Takashi Kaneta, Hiroki Shiba, Totaro Imasaka

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Enantiomeric amino acids were labeled with a cyanine derivative (Cy5) for selective determination by capillary electrophoresis combined with diode laser-induced fluorometry. Cyanine-labeled glutamic acid has a high negative charge so that it was difficult to detect by conventional capillary zone electrophoresis due to its electrophoretic mobility being greater than the electroosmotic mobility. Thus, cyclodextrin-modified capillary gel electrophoresis using replaceable poly(vinyl-pyrrolidone) (PVP), which effectively suppresses the electroosmotic flow, was employed to separate Cy5- labeled amino acid enantiomers. The separation of six amino acid enantiomers could be achieved by using 1% PVP solution containing 70 mM γ-cyclodextrin. It was also shown that PVP does not affect the selectivity of optical isomers.

Original languageEnglish
Pages (from-to)295-300
Number of pages6
JournalJournal of Chromatography A
Volume805
Issue number1-2
DOIs
Publication statusPublished - May 1 1998
Externally publishedYes

Keywords

  • Amino acids
  • Cyanine labelling
  • Derivatization, electrophoresis
  • Enantiomer separation
  • Poly(vinylpyrrolidone)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Determination of cyanine-labeled amino acid enantiomers by cyclodextrin- modified capillary gel electrophoresis combined with diode laser fluorescence detection'. Together they form a unique fingerprint.

Cite this