Determination of cyanine-labeled amino acid enantiomers by cyclodextrin- modified capillary gel electrophoresis combined with diode laser fluorescence detection

Takashi Kaneta, Hiroki Shiba, Totaro Imasaka

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Enantiomeric amino acids were labeled with a cyanine derivative (Cy5) for selective determination by capillary electrophoresis combined with diode laser-induced fluorometry. Cyanine-labeled glutamic acid has a high negative charge so that it was difficult to detect by conventional capillary zone electrophoresis due to its electrophoretic mobility being greater than the electroosmotic mobility. Thus, cyclodextrin-modified capillary gel electrophoresis using replaceable poly(vinyl-pyrrolidone) (PVP), which effectively suppresses the electroosmotic flow, was employed to separate Cy5- labeled amino acid enantiomers. The separation of six amino acid enantiomers could be achieved by using 1% PVP solution containing 70 mM γ-cyclodextrin. It was also shown that PVP does not affect the selectivity of optical isomers.

Original languageEnglish
Pages (from-to)295-300
Number of pages6
JournalJournal of Chromatography A
Volume805
Issue number1-2
DOIs
Publication statusPublished - May 1 1998
Externally publishedYes

Fingerprint

Semiconductor Lasers
Enantiomers
Cyclodextrins
Capillary Electrophoresis
Electrophoresis
Semiconductor lasers
Fluorescence
Gels
Amino Acids
Electroosmosis
Pyrrolidinones
Capillary electrophoresis
Fluorometry
Electrophoretic mobility
Isomers
Glutamic Acid
Derivatives
cyanine dye 5

Keywords

  • Amino acids
  • Cyanine labelling
  • Derivatization, electrophoresis
  • Enantiomer separation
  • Poly(vinylpyrrolidone)

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Determination of cyanine-labeled amino acid enantiomers by cyclodextrin- modified capillary gel electrophoresis combined with diode laser fluorescence detection. / Kaneta, Takashi; Shiba, Hiroki; Imasaka, Totaro.

In: Journal of Chromatography A, Vol. 805, No. 1-2, 01.05.1998, p. 295-300.

Research output: Contribution to journalArticle

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