Abstract
Enantiomeric amino acids were labeled with a cyanine derivative (Cy5) for selective determination by capillary electrophoresis combined with diode laser-induced fluorometry. Cyanine-labeled glutamic acid has a high negative charge so that it was difficult to detect by conventional capillary zone electrophoresis due to its electrophoretic mobility being greater than the electroosmotic mobility. Thus, cyclodextrin-modified capillary gel electrophoresis using replaceable poly(vinyl-pyrrolidone) (PVP), which effectively suppresses the electroosmotic flow, was employed to separate Cy5- labeled amino acid enantiomers. The separation of six amino acid enantiomers could be achieved by using 1% PVP solution containing 70 mM γ-cyclodextrin. It was also shown that PVP does not affect the selectivity of optical isomers.
| Original language | English |
|---|---|
| Pages (from-to) | 295-300 |
| Number of pages | 6 |
| Journal | Journal of Chromatography A |
| Volume | 805 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - May 1 1998 |
| Externally published | Yes |
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Keywords
- Amino acids
- Cyanine labelling
- Derivatization, electrophoresis
- Enantiomer separation
- Poly(vinylpyrrolidone)
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Determination of cyanine-labeled amino acid enantiomers by cyclodextrin- modified capillary gel electrophoresis combined with diode laser fluorescence detection. / Kaneta, Takashi; Shiba, Hiroki; Imasaka, Totaro.
In: Journal of Chromatography A, Vol. 805, No. 1-2, 01.05.1998, p. 295-300.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determination of cyanine-labeled amino acid enantiomers by cyclodextrin- modified capillary gel electrophoresis combined with diode laser fluorescence detection
AU - Kaneta, Takashi
AU - Shiba, Hiroki
AU - Imasaka, Totaro
PY - 1998/5/1
Y1 - 1998/5/1
N2 - Enantiomeric amino acids were labeled with a cyanine derivative (Cy5) for selective determination by capillary electrophoresis combined with diode laser-induced fluorometry. Cyanine-labeled glutamic acid has a high negative charge so that it was difficult to detect by conventional capillary zone electrophoresis due to its electrophoretic mobility being greater than the electroosmotic mobility. Thus, cyclodextrin-modified capillary gel electrophoresis using replaceable poly(vinyl-pyrrolidone) (PVP), which effectively suppresses the electroosmotic flow, was employed to separate Cy5- labeled amino acid enantiomers. The separation of six amino acid enantiomers could be achieved by using 1% PVP solution containing 70 mM γ-cyclodextrin. It was also shown that PVP does not affect the selectivity of optical isomers.
AB - Enantiomeric amino acids were labeled with a cyanine derivative (Cy5) for selective determination by capillary electrophoresis combined with diode laser-induced fluorometry. Cyanine-labeled glutamic acid has a high negative charge so that it was difficult to detect by conventional capillary zone electrophoresis due to its electrophoretic mobility being greater than the electroosmotic mobility. Thus, cyclodextrin-modified capillary gel electrophoresis using replaceable poly(vinyl-pyrrolidone) (PVP), which effectively suppresses the electroosmotic flow, was employed to separate Cy5- labeled amino acid enantiomers. The separation of six amino acid enantiomers could be achieved by using 1% PVP solution containing 70 mM γ-cyclodextrin. It was also shown that PVP does not affect the selectivity of optical isomers.
KW - Amino acids
KW - Cyanine labelling
KW - Derivatization, electrophoresis
KW - Enantiomer separation
KW - Poly(vinylpyrrolidone)
UR - http://www.scopus.com/inward/record.url?scp=0032076470&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032076470&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(98)00023-5
DO - 10.1016/S0021-9673(98)00023-5
M3 - Article
AN - SCOPUS:0032076470
VL - 805
SP - 295
EP - 300
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - 1-2
ER -