Detection of varicella-zoster virus dna by field-inversion gel electrophoresis

Yujiro Arao, M. Yoshida, Z. L. Bai, Y. Kori, A. Nakatsukasa, Y. Takei, K. Aoji, Masao Yamada, F. Uno, K. Miyoshi, S. Nii

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A new method for detection of varicella-zoster virus (VZV) DNA using field-inversion gel electrophoresis (FIGE) was devised. VZV-genomic DNA could be differentiated from the host cell DNA of human embryonic lung (HEL) fibroblasts infected with VZV under electrophoretic conditions allowing resolution of linear and double-stranded DNAs in the 49-230 kilobase pairs (Kb) range. The detection of VZV-genomic DNA from infected HEL cells was successful regardless of whether the VZV was a laboratory strain, live vaccine strain, or fresh isolate. Under the same electrophoretic conditions, DNA of VZV-infected HEL cells could be clearly differentiated from DNA obtained from HEL cells infected with herpes simplex virus type 1 (HSV-1), type 2 (HSV-2), or human cytomegalovirus (HCMV). Furthermore, VZV genomic DNA could be detected from as small a sample as 1.9 x 104 VZV-infected HEL cells. Finally, we could detect VZV genomic DNA from 10 samples of vesicle tissue (blister lids, each about 1-4 mm2) and one sample of vesicle fluid (about 5 μl) obtained from patients diagnosed as having herpes-zoster. The results of this study indicate that FIGE is a simple and promising method for the detection of VZV from clinical materials as well as infected in vitro cultured cells.

Original languageEnglish
Pages (from-to)269-282
Number of pages14
JournalMicrobiology and Immunology
Volume34
Issue number3
Publication statusPublished - 1990

Fingerprint

Human Herpesvirus 3
Pulsed Field Gel Electrophoresis
DNA
Lung
Human Herpesvirus 2
Herpes Zoster
Human Herpesvirus 1
Blister
Cytomegalovirus
Cultured Cells
Vaccines
Fibroblasts

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

Cite this

Arao, Y., Yoshida, M., Bai, Z. L., Kori, Y., Nakatsukasa, A., Takei, Y., ... Nii, S. (1990). Detection of varicella-zoster virus dna by field-inversion gel electrophoresis. Microbiology and Immunology, 34(3), 269-282.

Detection of varicella-zoster virus dna by field-inversion gel electrophoresis. / Arao, Yujiro; Yoshida, M.; Bai, Z. L.; Kori, Y.; Nakatsukasa, A.; Takei, Y.; Aoji, K.; Yamada, Masao; Uno, F.; Miyoshi, K.; Nii, S.

In: Microbiology and Immunology, Vol. 34, No. 3, 1990, p. 269-282.

Research output: Contribution to journalArticle

Arao, Y, Yoshida, M, Bai, ZL, Kori, Y, Nakatsukasa, A, Takei, Y, Aoji, K, Yamada, M, Uno, F, Miyoshi, K & Nii, S 1990, 'Detection of varicella-zoster virus dna by field-inversion gel electrophoresis', Microbiology and Immunology, vol. 34, no. 3, pp. 269-282.
Arao Y, Yoshida M, Bai ZL, Kori Y, Nakatsukasa A, Takei Y et al. Detection of varicella-zoster virus dna by field-inversion gel electrophoresis. Microbiology and Immunology. 1990;34(3):269-282.
Arao, Yujiro ; Yoshida, M. ; Bai, Z. L. ; Kori, Y. ; Nakatsukasa, A. ; Takei, Y. ; Aoji, K. ; Yamada, Masao ; Uno, F. ; Miyoshi, K. ; Nii, S. / Detection of varicella-zoster virus dna by field-inversion gel electrophoresis. In: Microbiology and Immunology. 1990 ; Vol. 34, No. 3. pp. 269-282.
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