Detection of thermospermine and spermine by HPLC in plants

Taku Takahashi, Ayaka Takano, Jun Ichi Kakehi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Thermospermine, a structural isomer of spermine, is widely spread in the plant kingdom and has recently been shown to play a key role in the repression of xylem differentiation in vascular plants. However, a standard high-performance liquid chromatography (HPLC) protocol for detecting polyamines as their dansyl derivative cannot distinguish themospermine from spermine. These isomers become separated from each other after benzoylation. In this chapter, we describe a simple protocol for extraction, benzoylation, and HPLC detection of thermospermine and spermine with other polyamines from plant material.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages69-73
Number of pages5
Volume1694
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1694
ISSN (Print)1064-3745

Keywords

  • Arabidopsis
  • Benzoylation
  • HPLC
  • Spermine
  • Thermospermine

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Takahashi, T., Takano, A., & Kakehi, J. I. (2018). Detection of thermospermine and spermine by HPLC in plants. In Methods in Molecular Biology (Vol. 1694, pp. 69-73). (Methods in Molecular Biology; Vol. 1694). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7398-9_6