Detection of the silkworm-pathogenic virus genomes by PCR.

Seiji Kageyasu, Tohru Hayakawa, Haruhiko Isawa, Shinichiro Asano, Ken Sahara, Toshihiko Iizuka, Hisanori Bando

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The five viruses, Bombyx mori densonucleosis virus type 1 (BmDNV-1), - type 2 (BmDNV-2), Bombyx mori nuclear polyhedrosis virus (BmNPV), Bombyx mori cytoplasmic polyhedrosis virus (BmCPV), and Infectious flacherie virus (IFV), are the agents causing fetal disease of the silkworm. The aim of this study is to establish a simple and highly sensitive PCR system for detection of all the viruses. A combination of the RT-PCR and the nested PCR was found to be effective to amplify DNA and/or RNA virus genome sequences in a reaction maintaining high sensitivity and specificity. In this report, the primer design and the condition of the PCR for the detection of all the viruses are described.

Original languageEnglish
Pages (from-to)477-483
Number of pages7
JournalJournal of Sericultural Science of Japan
Volume66
Issue number6
DOIs
Publication statusPublished - Jan 1 1997
Externally publishedYes

Fingerprint

Bombyx
silkworms
Viruses
Genes
Genome
Polymerase Chain Reaction
viruses
genome
Bombyx mori
Infectious flacherie virus
Bombyx mori nucleopolyhedrovirus
Cypovirus
Reoviridae
Fetal Diseases
Nucleopolyhedrovirus
DNA Viruses
RNA Viruses
reverse transcriptase polymerase chain reaction
Silkworm
Virus

Keywords

  • BmCPV
  • BmDNV
  • BmNPV
  • IFV
  • nested PCR
  • RT-PCR

ASJC Scopus subject areas

  • Polymers and Plastics

Cite this

Detection of the silkworm-pathogenic virus genomes by PCR. / Kageyasu, Seiji; Hayakawa, Tohru; Isawa, Haruhiko; Asano, Shinichiro; Sahara, Ken; Iizuka, Toshihiko; Bando, Hisanori.

In: Journal of Sericultural Science of Japan, Vol. 66, No. 6, 01.01.1997, p. 477-483.

Research output: Contribution to journalArticle

Kageyasu, S, Hayakawa, T, Isawa, H, Asano, S, Sahara, K, Iizuka, T & Bando, H 1997, 'Detection of the silkworm-pathogenic virus genomes by PCR.', Journal of Sericultural Science of Japan, vol. 66, no. 6, pp. 477-483. https://doi.org/10.11416/kontyushigen1930.66.477
Kageyasu, Seiji ; Hayakawa, Tohru ; Isawa, Haruhiko ; Asano, Shinichiro ; Sahara, Ken ; Iizuka, Toshihiko ; Bando, Hisanori. / Detection of the silkworm-pathogenic virus genomes by PCR. In: Journal of Sericultural Science of Japan. 1997 ; Vol. 66, No. 6. pp. 477-483.
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