Detection of t(14;18)(q32;q21) in hyperdiploid cells by fluorescence in situ hybridization in a patient with Hodgkin disease

I. Miura, A. Tamura, M. Taniwaki, S. Nakamura, H. Nakamine, Tadashi Yoshino, R. Ichinohasama, A. B. Miura

Research output: Contribution to journalArticle

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Abstract

The most frequent nonrandom chromosome rearrangements in B-cell non-Hodgkin lymphoma (NHL) is the t(14;18)(q32;q21) found in follicular lymphomas. The t(14;18) in Hodgkin disease (HD) was rarely observed using cytogenetic techniques. Although Southern blot analysis failed to demonstrate the t(14;18), there have been conflicting reports concerning the occurrence of the translocation using polymerase chain reaction (PCR) methods in HD. In some HD tissues, the translocation might be derived from background lymphocytes rather than Hodgkin and Reed-Sternberg (HRS) cells, because B-cells with t(14;18) are regularly generated in normal individuals. However, the cells bearing the translocation have remained unidentified. We describe a patient with HD who showed t(14;18) in hyperdiploid cells using fluorescence in situ hybridization (FISH) and HRS cells which were strongly positive for BCL2 by immunohistochemistry. These findings suggest that HRS cells may have a t(14;18).

Original languageEnglish
Pages (from-to)97-101
Number of pages5
JournalCancer Genetics and Cytogenetics
Volume123
Issue number2
DOIs
Publication statusPublished - 2000

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Polyploidy
Fluorescence In Situ Hybridization
Hodgkin Disease
Reed-Sternberg Cells
Follicular Lymphoma
Cytogenetic Analysis
B-Cell Lymphoma
Southern Blotting
Non-Hodgkin's Lymphoma
B-Lymphocytes
Chromosomes
Immunohistochemistry
Lymphocytes
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

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Detection of t(14;18)(q32;q21) in hyperdiploid cells by fluorescence in situ hybridization in a patient with Hodgkin disease. / Miura, I.; Tamura, A.; Taniwaki, M.; Nakamura, S.; Nakamine, H.; Yoshino, Tadashi; Ichinohasama, R.; Miura, A. B.

In: Cancer Genetics and Cytogenetics, Vol. 123, No. 2, 2000, p. 97-101.

Research output: Contribution to journalArticle

Miura, I. ; Tamura, A. ; Taniwaki, M. ; Nakamura, S. ; Nakamine, H. ; Yoshino, Tadashi ; Ichinohasama, R. ; Miura, A. B. / Detection of t(14;18)(q32;q21) in hyperdiploid cells by fluorescence in situ hybridization in a patient with Hodgkin disease. In: Cancer Genetics and Cytogenetics. 2000 ; Vol. 123, No. 2. pp. 97-101.
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AU - Miura, I.

AU - Tamura, A.

AU - Taniwaki, M.

AU - Nakamura, S.

AU - Nakamine, H.

AU - Yoshino, Tadashi

AU - Ichinohasama, R.

AU - Miura, A. B.

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AB - The most frequent nonrandom chromosome rearrangements in B-cell non-Hodgkin lymphoma (NHL) is the t(14;18)(q32;q21) found in follicular lymphomas. The t(14;18) in Hodgkin disease (HD) was rarely observed using cytogenetic techniques. Although Southern blot analysis failed to demonstrate the t(14;18), there have been conflicting reports concerning the occurrence of the translocation using polymerase chain reaction (PCR) methods in HD. In some HD tissues, the translocation might be derived from background lymphocytes rather than Hodgkin and Reed-Sternberg (HRS) cells, because B-cells with t(14;18) are regularly generated in normal individuals. However, the cells bearing the translocation have remained unidentified. We describe a patient with HD who showed t(14;18) in hyperdiploid cells using fluorescence in situ hybridization (FISH) and HRS cells which were strongly positive for BCL2 by immunohistochemistry. These findings suggest that HRS cells may have a t(14;18).

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