Detection of serum miRNAs affecting liver apoptosis in a periodontitis rat model

Yoshio Sugiura, Toshiki Yoneda, Kohei Fujimori, Takayuki Maruyama, Hisataka Miyai, Terumasa Kobayashi, Daisuke Ekuni, Takaaki Tomofuji, Manabu Morita

Research output: Contribution to journalArticle

Abstract

Background/Aim: miRNA molecules have been attracting attention as genetic modifiers between organs. We examined the relationship between serum miRNA and targeted liver mRNA profiles in a periodontitis rat model, and the influence of periodontitis on the liver. Materials and Methods: Male Wistar rats (n=16, 8 weeks old) were randomly divided into two groups (8 rats each): control and periodontitis (ligature placement for 4 weeks). Serum miRNA and liver mRNA profiles were compared. Results: Periodontal destruction and hepatocyte apoptosis were induced in the periodontitis group. Microarray analysis indicated that 52 serum miRNAs and 33 liver mRNAs were expressed with a >1.5-fold change (FC) and a >2.0-FC (p<0.05), respectively, between the two groups. From the miRNA target genes, 12 genes equivalented to liver mRNAs with a >2.0-FC, among which, Hyou1, Chac1, and Bloc1s3 have apoptotic functions in our model. miRNAs upstream of these 3 mRNAs are miR-3591, miR-181a-2-3p and miR-6321. Conclusion: miR-3591, miR-181a-2-3p and miR-6321 induced hepatocyte apoptosis in our periodontitis rat model.

Original languageEnglish
Pages (from-to)117-123
Number of pages7
JournalIn Vivo
Volume34
Issue number1
DOIs
Publication statusPublished - Jan 1 2020

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Keywords

  • Liver
  • MicroRNAs
  • Periodontitis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

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