TY - JOUR
T1 - Detection of serum miRNAs affecting liver apoptosis in a periodontitis rat model
AU - Sugiura, Yoshio
AU - Yoneda, Toshiki
AU - Fujimori, Kohei
AU - Maruyama, Takayuki
AU - Miyai, Hisataka
AU - Kobayashi, Terumasa
AU - Ekuni, Daisuke
AU - Tomofuji, Takaaki
AU - Morita, Manabu
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (16K11855) from the Japan Society for the Promotion of Science, Tokyo, Japan.
Publisher Copyright:
© 2020 International Institute of Anticancer Research. All rights reserved.
PY - 2020
Y1 - 2020
N2 - Background/Aim: miRNA molecules have been attracting attention as genetic modifiers between organs. We examined the relationship between serum miRNA and targeted liver mRNA profiles in a periodontitis rat model, and the influence of periodontitis on the liver. Materials and Methods: Male Wistar rats (n=16, 8 weeks old) were randomly divided into two groups (8 rats each): control and periodontitis (ligature placement for 4 weeks). Serum miRNA and liver mRNA profiles were compared. Results: Periodontal destruction and hepatocyte apoptosis were induced in the periodontitis group. Microarray analysis indicated that 52 serum miRNAs and 33 liver mRNAs were expressed with a >1.5-fold change (FC) and a >2.0-FC (p<0.05), respectively, between the two groups. From the miRNA target genes, 12 genes equivalented to liver mRNAs with a >2.0-FC, among which, Hyou1, Chac1, and Bloc1s3 have apoptotic functions in our model. miRNAs upstream of these 3 mRNAs are miR-3591, miR-181a-2-3p and miR-6321. Conclusion: miR-3591, miR-181a-2-3p and miR-6321 induced hepatocyte apoptosis in our periodontitis rat model.
AB - Background/Aim: miRNA molecules have been attracting attention as genetic modifiers between organs. We examined the relationship between serum miRNA and targeted liver mRNA profiles in a periodontitis rat model, and the influence of periodontitis on the liver. Materials and Methods: Male Wistar rats (n=16, 8 weeks old) were randomly divided into two groups (8 rats each): control and periodontitis (ligature placement for 4 weeks). Serum miRNA and liver mRNA profiles were compared. Results: Periodontal destruction and hepatocyte apoptosis were induced in the periodontitis group. Microarray analysis indicated that 52 serum miRNAs and 33 liver mRNAs were expressed with a >1.5-fold change (FC) and a >2.0-FC (p<0.05), respectively, between the two groups. From the miRNA target genes, 12 genes equivalented to liver mRNAs with a >2.0-FC, among which, Hyou1, Chac1, and Bloc1s3 have apoptotic functions in our model. miRNAs upstream of these 3 mRNAs are miR-3591, miR-181a-2-3p and miR-6321. Conclusion: miR-3591, miR-181a-2-3p and miR-6321 induced hepatocyte apoptosis in our periodontitis rat model.
KW - Liver
KW - MicroRNAs
KW - Periodontitis
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U2 - 10.21873/invivo.11752
DO - 10.21873/invivo.11752
M3 - Article
C2 - 31882470
AN - SCOPUS:85077267431
SN - 0258-851X
VL - 34
SP - 117
EP - 123
JO - In Vivo
JF - In Vivo
IS - 1
ER -