Detection of replicatire intermediate with closed terminus of Bombyx densonucleosis virus

Tohru Hayakawa, S. Asano, K. Sahara, T. Iizuka, H. Bando

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Two kinds of Bombyx densonucleosis virus (BmDNV), BmDNV-1 and 2, have been isolated from sericultural farms in Japan or China. These viruses are classified into the family Parvoviridae because of the small spherical virus particle containing a single-stranded linear DNA genome. Recent studies on the genome structure of these viruses suggested that BmDNV-2 was a new type of virus with unique replication mechanism, though that of BmDNV-1 was similar to parvoviruses. However, details about the replication mechanism of BmDNVs have not been reported so far. Here, in order to elucidate the difference on replication mechanism between BmDNVs and parvoviruses, we analyzed the structure of the replicative intermediate (RI) of BmDNV DNAs by PCR using specific primers designed for detection of RI with closed terminal structure (RI-CT) which is expected to be formed by replication with self-priming mechanism. PCR using the DNA from the cells infected with BmDNV-1 could detect the expected DNA fragment, showing the existence of RI-CT. On the other hand, no fragment could be amplified from the virion DNA of the BmDNVs and the DNA extracted from BmDNV-2-infected cells, respectively. These observations strongly suggested that the BmDNV-1 replicates with the 'self-priming and hairpin-transfer' mechanism similar to the human parvoviruses, while BmDNV-2 does not.

Original languageEnglish
Pages (from-to)393-399
Number of pages7
JournalArchives of Virology
Volume142
Issue number2
DOIs
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Bombyx
Viruses
Parvovirus
DNA
Virion
Parvoviridae
Genome
Viral Structures
Polymerase Chain Reaction
DNA Viruses
Single-Stranded DNA
China
Japan

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Detection of replicatire intermediate with closed terminus of Bombyx densonucleosis virus. / Hayakawa, Tohru; Asano, S.; Sahara, K.; Iizuka, T.; Bando, H.

In: Archives of Virology, Vol. 142, No. 2, 1997, p. 393-399.

Research output: Contribution to journalArticle

Hayakawa, Tohru ; Asano, S. ; Sahara, K. ; Iizuka, T. ; Bando, H. / Detection of replicatire intermediate with closed terminus of Bombyx densonucleosis virus. In: Archives of Virology. 1997 ; Vol. 142, No. 2. pp. 393-399.
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