Detection of QTLs to reduce cadmium content in rice grains using LAC23/Koshihikari chromosome segment substitution lines

Tadashi Abe, Yasunori Nonoue, Nozomi Ono, Motoyasu Omoteno, Masato Kuramata, Shuichi Fukuoka, Toshio Yamamoto, Masahiro Yano, Satoru Ishikawa

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

To advance the identification of quantitative trait loci (QTLs) to reduce Cd content in rice (Oryza sativa L.) grains and breed low-Cd cultivars, we developed a novel population consisting of 46 chromosome segment substitution lines (CSSLs) in which donor segments of LAC23, a cultivar reported to have a low grain Cd content, were substituted into the Koshihikari genetic background. The parental cultivars and 32 CSSLs (the minimum set required for whole-genome coverage) were grown in two fields with different natural levels of soil Cd. QTL mapping by single-marker analysis using ANOVA indicated that eight chromosomal regions were associated with grain Cd content and detected a major QTL (qlGCd3) with a high F-test value in both fields (F = 9.19 and 5.60) on the long arm of chromosome 3. The LAC23 allele at qlGCd3 was associated with reduced grain Cd levels and appeared to reduce Cd transport from the shoots to the grains. Fine substitution mapping delimited qlGCd3 to a 3.5-Mbp region. Our results suggest that the low-Cd trait of LAC23 is controlled by multiple QTLs, and qlGCd3 is a promising candidate QTL to reduce the Cd level of rice grain.

Original languageEnglish
Pages (from-to)284-291
Number of pages8
JournalBreeding Science
Volume63
Issue number3
DOIs
Publication statusPublished - Oct 8 2013
Externally publishedYes

Keywords

  • Chromosome segment substitution lines (CSSLs)
  • Low cadmium (Cd)
  • Paddy field
  • Quantitative trait locus (QTL)
  • Rice grain

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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