Detection of QTLs for salt tolerance in Asian barley (Hordeum vulgare l.) by association analysis with SNP markers

Hanen Sbei, Kazuhiro Sato, Tariq Shehzad, Moncef Harrabi, Kazutoshi Okuno

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Two hundred ninety-six Asian barley (Hordeum vulgare L.) accessions were assessed to detect QTLs underlying salt tolerance by association analysis using a 384 single nucleotide polymorphism (SNP) marker system. The experiment was laid out at the seedling stage in a hydroponic solution under control and 250 mM NaCl solution with three replications of four plants each. Salt tolerance was assessed by leaf injury score (LIS) and salt tolerance indices (STIs) of the number of leaves (NL), shoot length (SL), root length (RL), shoot dry weight (SDW) and root dry weight (RDW). LIS was scored from 1 to 5 according to the severity of necrosis and chlorosis observed on leaves. There was a wide variation in salt tolerance among Asian barley accessions. LIS and STI (SDW) were the most suitable traits for screening salt tolerance. Association was estimated between markers and traits to detect QTLs for LIS and STI (SDW). Seven significant QTLs were located on chromosomes 1H (2 QTLs), 2H (2 QTLs), 3H (1 QTL), 4H (1 QTL) and 5H (1 QTL). Five QTLs were associated with LIS and 2 QTLs with STI (SDW). Two QTLs associated with LIS were newly identified on chromosomes 3H and 4H.

Original languageEnglish
Pages (from-to)378-388
Number of pages11
JournalBreeding Science
Volume64
Issue number4
DOIs
Publication statusPublished - Jan 10 2015

Fingerprint

Salt-Tolerance
Hordeum
salt tolerance
Hordeum vulgare
single nucleotide polymorphism
Single Nucleotide Polymorphism
quantitative trait loci
barley
Wounds and Injuries
Weights and Measures
leaves
shoots
Hydroponics
Hypochromic Anemia
Chromosomes, Human, Pair 2
Seedlings
chromosomes
Necrosis
Chromosomes
chlorosis

Keywords

  • Association mapping
  • Hordeum vulgare L
  • Leaf injury score
  • Quantitative trait loci
  • Salt tolerance
  • Salt tolerance index
  • SNP markers

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Detection of QTLs for salt tolerance in Asian barley (Hordeum vulgare l.) by association analysis with SNP markers. / Sbei, Hanen; Sato, Kazuhiro; Shehzad, Tariq; Harrabi, Moncef; Okuno, Kazutoshi.

In: Breeding Science, Vol. 64, No. 4, 10.01.2015, p. 378-388.

Research output: Contribution to journalArticle

Sbei, Hanen ; Sato, Kazuhiro ; Shehzad, Tariq ; Harrabi, Moncef ; Okuno, Kazutoshi. / Detection of QTLs for salt tolerance in Asian barley (Hordeum vulgare l.) by association analysis with SNP markers. In: Breeding Science. 2015 ; Vol. 64, No. 4. pp. 378-388.
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