Detection of platypus-type l/d-peptide isomerase activity in aqueous extracts of papaya fruit

Kensuke Arakawa, Jennifer M S Koh, Ben Crossett, Allan M. Torres, Philip W. Kuchel

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Peptide isomerase catalyses the post-translational isomerisation of the l- to the d-form of an amino acid residue around the N/C-termini of substrate peptides. To date, some peptide isomerases have been found in a limited number of animal secretions and cells. We show here that papaya extracts have weak peptide isomerase activity. The activity was detected in each 30-100 kDa fraction of the flesh and the seed extracts of unripe and ripe papaya fruit. The definitive activity was confirmed in the ripe papaya extracts, but even then it was much less active than that of the other peptide isomerases previously reported. The activity was markedly inhibited by methanol, and partly so by amastatin and diethyl pyrocarbonate. This is the first report of peptide isomerase activity in a plant and suggests that perhaps every living organism may have some peptide isomerase activity.

Original languageEnglish
Pages (from-to)1659-1665
Number of pages7
JournalBiotechnology Letters
Volume34
Issue number9
DOIs
Publication statusPublished - Oct 2012

Fingerprint

Platypus
Carica
Isomerases
Fruits
Peptides
Fruit
Diethyl Pyrocarbonate
Isomerization
Methanol
Seed
Amino acids
Seeds
Animals
Amino Acids

Keywords

  • Carica papaya L.
  • d-Amino acid
  • Peptide isomerase
  • Peptidyl aminoacyl l/d-isomerase

ASJC Scopus subject areas

  • Biotechnology

Cite this

Detection of platypus-type l/d-peptide isomerase activity in aqueous extracts of papaya fruit. / Arakawa, Kensuke; Koh, Jennifer M S; Crossett, Ben; Torres, Allan M.; Kuchel, Philip W.

In: Biotechnology Letters, Vol. 34, No. 9, 10.2012, p. 1659-1665.

Research output: Contribution to journalArticle

Arakawa, Kensuke ; Koh, Jennifer M S ; Crossett, Ben ; Torres, Allan M. ; Kuchel, Philip W. / Detection of platypus-type l/d-peptide isomerase activity in aqueous extracts of papaya fruit. In: Biotechnology Letters. 2012 ; Vol. 34, No. 9. pp. 1659-1665.
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