Detection of occult tumor cells in peripheral blood from patients with small cell lung cancer by reverse transcriptase-polymerase chain reaction

Akihiro Bessho, Masahiro Tabata, Katsuyuki Kiura, Ichiro Takata, Takuya Nagata, Nobukazu Fujimoto, Kouichi Kunisada, Hiroshi Ueoka, Mine Harada

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The reverse transcriptase-polymerase chain reaction (RT-PCR) of tumor-specific or -associated genes is a sensitive assay for detecting a minimal number of tumor cells in peripheral blood (PB) or bone marrow (BM). In this study, we determined whether mRNA of bombesin receptors is detectable in PB or peripheral blood progenitor cell (PBPC) samples from patients with small cell lung cancer. Among three bombesin-like peptide receptors, we used the neuromedin B receptor (NMB-R) gene as a target, because of the most frequent expression on SCLC cell lines. The lower limit of detection was one tumor cell in one million normal PB cells and there was no detection in normal PB or BM cells unlike a cytokeratin 19 gene. The NMB-R gene was detected in 14 (31.8%) of 44 PB samples from patients with SCLC at diagnosis and 2 (15.4%) of 13 samples of PBPC collected during a recovery phase after chemotherapy followed by administration of G-CSF (filgrastim). At diagnosis, patients whose PB was positive for the NMB-R gene had a significantly shorter survival than those who were negative. Our observation suggests that this assay may be useful in diagnosing metastatic disease and monitoring minimal residual disease in patients with SCLC.

Original languageEnglish
Pages (from-to)1149-1154
Number of pages6
JournalAnticancer Research
Volume20
Issue number2 B
Publication statusPublished - 2000

Fingerprint

Bombesin Receptors
Small Cell Lung Carcinoma
Reverse Transcriptase Polymerase Chain Reaction
Blood Cells
Genes
Neoplasms
Stem Cells
Keratin-19
Bombesin
Peptide Receptors
Residual Neoplasm
Granulocyte Colony-Stimulating Factor
Bone Marrow Cells
Limit of Detection
Cell Count
Bone Marrow
Observation
Drug Therapy
Cell Line
Messenger RNA

Keywords

  • Bombesin
  • GRP-receptor
  • Molecular detection
  • NMB-receptor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Detection of occult tumor cells in peripheral blood from patients with small cell lung cancer by reverse transcriptase-polymerase chain reaction. / Bessho, Akihiro; Tabata, Masahiro; Kiura, Katsuyuki; Takata, Ichiro; Nagata, Takuya; Fujimoto, Nobukazu; Kunisada, Kouichi; Ueoka, Hiroshi; Harada, Mine.

In: Anticancer Research, Vol. 20, No. 2 B, 2000, p. 1149-1154.

Research output: Contribution to journalArticle

Bessho, A, Tabata, M, Kiura, K, Takata, I, Nagata, T, Fujimoto, N, Kunisada, K, Ueoka, H & Harada, M 2000, 'Detection of occult tumor cells in peripheral blood from patients with small cell lung cancer by reverse transcriptase-polymerase chain reaction', Anticancer Research, vol. 20, no. 2 B, pp. 1149-1154.
Bessho, Akihiro ; Tabata, Masahiro ; Kiura, Katsuyuki ; Takata, Ichiro ; Nagata, Takuya ; Fujimoto, Nobukazu ; Kunisada, Kouichi ; Ueoka, Hiroshi ; Harada, Mine. / Detection of occult tumor cells in peripheral blood from patients with small cell lung cancer by reverse transcriptase-polymerase chain reaction. In: Anticancer Research. 2000 ; Vol. 20, No. 2 B. pp. 1149-1154.
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