Detection of novel orthoreovirus genomes in shrew (Crocidura hirta) and fruit bat (rousettus aegyptiacus)

Hayato Harima, Michihito Sasaki, Masahiro Kajihara, Akina Mori-Kajihara, Bernard M. Hang’ombe, Katendi Changula, Yasuko Orba, Hirohito Ogawa, Martin Simuunza, Reiko Yoshida, Aaron Mweene, Ayato Takada, Hirofumi Sawa

Research output: Contribution to journalComment/debate

Abstract

Orthoreoviruses have been indentified in several mammals, however, there is no information about orthoreoviruses in shrews. In this study, we screened wild animals in Zambia, including shrews, rodents, and bats for the detection of orthoreoviruses. Two orthoreovirus RNA genomes were detected from a shrew intestinal-contents (1/24) and a bat colon (1/96) sample by reverse-transcription (RT)-PCR targeting the RNA-dependent RNA polymerase gene of orthoreoviruses. Phylogenetic analyses revealed that each of the identified orthoreoviruses formed a distinct branch among members of the Orthoreovirus genus. This is the first report that shrews are susceptible to orthoreovirus infection. Our results suggest the existence of undiscovered orthoreoviruses in shrews and provide important information about the genetic diversity of orthoreoviruses.

Original languageEnglish
Pages (from-to)162-167
Number of pages6
JournalJournal of Veterinary Medical Science
Volume82
Issue number2
DOIs
Publication statusPublished - 2020
Externally publishedYes

Keywords

  • Crocidura hirta
  • Orthoreovirus
  • Rousettus aegyptiacus
  • Zambia

ASJC Scopus subject areas

  • veterinary(all)

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  • Cite this

    Harima, H., Sasaki, M., Kajihara, M., Mori-Kajihara, A., Hang’ombe, B. M., Changula, K., Orba, Y., Ogawa, H., Simuunza, M., Yoshida, R., Mweene, A., Takada, A., & Sawa, H. (2020). Detection of novel orthoreovirus genomes in shrew (Crocidura hirta) and fruit bat (rousettus aegyptiacus). Journal of Veterinary Medical Science, 82(2), 162-167. https://doi.org/10.1292/jvms.19-0424