Detection of novel orthoreovirus genomes in shrew (Crocidura hirta) and fruit bat (Rousettus aegyptiacus)

Hayato Harima, Michihito Sasaki, Masahiro Kajihara, Akina Mori-Kajihara, Bernard M Hang'ombe, Katendi Changula, Yasuko Orba, Hirohito Ogawa, Martin Simuunza, Reiko Yoshida, Aaron Mweene, Ayato Takada, Hirofumi Sawa

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Abstract

Orthoreoviruses have been indentified in several mammals, however, there is no information about orthoreoviruses in shrews. In this study, we screened wild animals in Zambia, including shrews, rodents, and bats for the detection of orthoreoviruses. Two orthoreovirus RNA genomes were detected from a shrew intestinal-contents (1/24) and a bat colon (1/96) sample by reverse-transcription (RT)-PCR targeting the RNA-dependent RNA polymerase gene of orthoreoviruses. Phylogenetic analyses revealed that each of the identified orthoreoviruses formed a distinct branch among members of the Orthoreovirus genus. This is the first report that shrews are susceptible to orthoreovirus infection. Our results suggest the existence of undiscovered orthoreoviruses in shrews and provide important information about the genetic diversity of orthoreoviruses.

Original languageEnglish
JournalThe Journal of veterinary medical science
DOIs
Publication statusE-pub ahead of print - Dec 20 2019
Externally publishedYes

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Harima, H., Sasaki, M., Kajihara, M., Mori-Kajihara, A., Hang'ombe, B. M., Changula, K., Orba, Y., Ogawa, H., Simuunza, M., Yoshida, R., Mweene, A., Takada, A., & Sawa, H. (2019). Detection of novel orthoreovirus genomes in shrew (Crocidura hirta) and fruit bat (Rousettus aegyptiacus). The Journal of veterinary medical science. https://doi.org/10.1292/jvms.19-0424