Detection of NO-induced DNA lesions by the modified aldehyde reactive probe (ARP) assay.

Toshiaki Nakano; Hiroaki Terato; Yoshihiro Yoshioka; Yoshihiko Ohyama; Kihei Kubo; Hiroshi Ide

Detection of NO-induced DNA lesions by the modified aldehyde reactive probe (ARP) assay.

Toshiaki Nakano, Hiroaki Terato, Yoshihiro Yoshioka, Yoshihiko Ohyama, Kihei Kubo, Hiroshi Ide

Research output: Contribution to journal › Article

Abstract

When DNA is exposed to NO or HNO2, oxanine (Oxa) is formed as a major guanine lesion. For highly sensitive detection of Oxa using ARP, a probe molecule for DNA damage detection, the reactivity of ARP to Oxa was examined. Oxa site-specifically embedded in an oligonucleotide reacted with ARP but it took relatively long time (ca. 24 h) to completely convert Oxa to an ARP-labeled form.

Original language	English
Pages (from-to)	239-240
Number of pages	2
Journal	Nucleic acids research. Supplement (2001)
Issue number	2
Publication status	Published - 2002
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

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Nakano, T., Terato, H., Yoshioka, Y., Ohyama, Y., Kubo, K., & Ide, H. (2002). Detection of NO-induced DNA lesions by the modified aldehyde reactive probe (ARP) assay. Nucleic acids research. Supplement (2001), (2), 239-240.

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abstract = "When DNA is exposed to NO or HNO2, oxanine (Oxa) is formed as a major guanine lesion. For highly sensitive detection of Oxa using ARP, a probe molecule for DNA damage detection, the reactivity of ARP to Oxa was examined. Oxa site-specifically embedded in an oligonucleotide reacted with ARP but it took relatively long time (ca. 24 h) to completely convert Oxa to an ARP-labeled form.",

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AU - Kubo, Kihei

AU - Ide, Hiroshi

PY - 2002

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