Abstract
When DNA is exposed to NO or HNO2, oxanine (Oxa) is formed as a major guanine lesion. For highly sensitive detection of Oxa using ARP, a probe molecule for DNA damage detection, the reactivity of ARP to Oxa was examined. Oxa site-specifically embedded in an oligonucleotide reacted with ARP but it took relatively long time (ca. 24 h) to completely convert Oxa to an ARP-labeled form.
| Original language | English |
|---|---|
| Pages (from-to) | 239-240 |
| Number of pages | 2 |
| Journal | Nucleic acids research. Supplement (2001) |
| Issue number | 2 |
| Publication status | Published - Jan 1 2002 |
| Externally published | Yes |
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Detection of NO-induced DNA lesions by the modified aldehyde reactive probe (ARP) assay. / Nakano, Toshiaki; Terato, Hiroaki; Yoshioka, Yoshihiro; Ohyama, Yoshihiko; Kubo, Kihei; Ide, Hiroshi.
In: Nucleic acids research. Supplement (2001), No. 2, 01.01.2002, p. 239-240.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of NO-induced DNA lesions by the modified aldehyde reactive probe (ARP) assay.
AU - Nakano, Toshiaki
AU - Terato, Hiroaki
AU - Yoshioka, Yoshihiro
AU - Ohyama, Yoshihiko
AU - Kubo, Kihei
AU - Ide, Hiroshi
PY - 2002/1/1
Y1 - 2002/1/1
N2 - When DNA is exposed to NO or HNO2, oxanine (Oxa) is formed as a major guanine lesion. For highly sensitive detection of Oxa using ARP, a probe molecule for DNA damage detection, the reactivity of ARP to Oxa was examined. Oxa site-specifically embedded in an oligonucleotide reacted with ARP but it took relatively long time (ca. 24 h) to completely convert Oxa to an ARP-labeled form.
AB - When DNA is exposed to NO or HNO2, oxanine (Oxa) is formed as a major guanine lesion. For highly sensitive detection of Oxa using ARP, a probe molecule for DNA damage detection, the reactivity of ARP to Oxa was examined. Oxa site-specifically embedded in an oligonucleotide reacted with ARP but it took relatively long time (ca. 24 h) to completely convert Oxa to an ARP-labeled form.
UR - http://www.scopus.com/inward/record.url?scp=0042819884&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0042819884&partnerID=8YFLogxK
M3 - Article
C2 - 12903194
AN - SCOPUS:0042819884
SP - 239
EP - 240
JO - Nucleic acids research. Supplement (2001)
JF - Nucleic acids research. Supplement (2001)
IS - 2
ER -