Detection of NO-induced DNA lesions by the modified aldehyde reactive probe (ARP) assay.

Toshiaki Nakano, Hiroaki Terato, Yoshihiro Yoshioka, Yoshihiko Ohyama, Kihei Kubo, Hiroshi Ide

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

When DNA is exposed to NO or HNO2, oxanine (Oxa) is formed as a major guanine lesion. For highly sensitive detection of Oxa using ARP, a probe molecule for DNA damage detection, the reactivity of ARP to Oxa was examined. Oxa site-specifically embedded in an oligonucleotide reacted with ARP but it took relatively long time (ca. 24 h) to completely convert Oxa to an ARP-labeled form.

Original languageEnglish
Pages (from-to)239-240
Number of pages2
JournalNucleic acids research. Supplement (2001)
Issue number2
Publication statusPublished - Jan 1 2002
Externally publishedYes

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DNA
Guanine
Oligonucleotides
DNA Damage
O-(biotinylcarbazoylmethyl)hydroxylamine
oxanine

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Detection of NO-induced DNA lesions by the modified aldehyde reactive probe (ARP) assay. / Nakano, Toshiaki; Terato, Hiroaki; Yoshioka, Yoshihiro; Ohyama, Yoshihiko; Kubo, Kihei; Ide, Hiroshi.

In: Nucleic acids research. Supplement (2001), No. 2, 01.01.2002, p. 239-240.

Research output: Contribution to journalArticle

Nakano, Toshiaki ; Terato, Hiroaki ; Yoshioka, Yoshihiro ; Ohyama, Yoshihiko ; Kubo, Kihei ; Ide, Hiroshi. / Detection of NO-induced DNA lesions by the modified aldehyde reactive probe (ARP) assay. In: Nucleic acids research. Supplement (2001). 2002 ; No. 2. pp. 239-240.
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