Detection of NO-induced DNA lesions by the modified aldehyde reactive probe (ARP) assay.

Toshiaki Nakano, Hiroaki Terato, Yoshihiro Yoshioka, Yoshihiko Ohyama, Kihei Kubo, Hiroshi Ide

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

When DNA is exposed to NO or HNO2, oxanine (Oxa) is formed as a major guanine lesion. For highly sensitive detection of Oxa using ARP, a probe molecule for DNA damage detection, the reactivity of ARP to Oxa was examined. Oxa site-specifically embedded in an oligonucleotide reacted with ARP but it took relatively long time (ca. 24 h) to completely convert Oxa to an ARP-labeled form.

Original languageEnglish
Pages (from-to)239-240
Number of pages2
JournalNucleic acids research. Supplement (2001)
Issue number2
Publication statusPublished - 2002
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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