Detection of neutrophils and possible involvement of interleukin-8 in diffuse lamellar keratitis after laser in situ keratomileusis

Naoko Asano-Kato, Ikuko Toda, Shigeto Shimmura, Tomoko Noda-Tsuruya, Kazumi Fukagawa, Masaru Yoshinaga, Akihiro Matsukawa, Kazuo Tsubota

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Purpose: To investigate the cell populations in diffuse lamellar keratitis (DLK) infiltration after laser in situ keratomileusis and the possible mechanism underlying the infiltration. Setting: Department of Ophthalmology, Tokyo Dental College, Chiba, Japan. Methods: To develop DLK in rabbit eyes, 25 μL of lipopolysaccharide (LPS) solution at a concentration of 50 μg/mL was applied to the stromal bed beneath corneal flaps. For control rabbits, phosphate-buffered saline was applied. Postoperative examination by slitlamp microscopy was performed for 3 days after surgery. Rabbit eyes were excised and examined for histopathology with hematoxylin and eosin staining. Immunohistochemical analysis for interleukin (IL)-8 was performed. Results: Diffuse lamellar keratitis-like inflammation composed mainly of neutrophils was reproduced by LPS instillation in rabbit eyes. In eyes with severe inflammation, IL-8 immunoreactivity was found in the stromal keratocytes and infiltrating neutrophils. Conclusions: The major cell type in the DLK infiltration induced by LPS instillation in rabbit eyes was the neutrophil. Interleukin-8, a prototype of CXC chemokine produced by keratocytes and neutrophils, may contribute to the development of DLK.

Original languageEnglish
Pages (from-to)1996-2000
Number of pages5
JournalJournal of Cataract and Refractive Surgery
Volume29
Issue number10
DOIs
Publication statusPublished - Oct 2003
Externally publishedYes

Fingerprint

Laser In Situ Keratomileusis
Keratitis
Interleukin-8
Neutrophils
Rabbits
Lipopolysaccharides
Inflammation
CXC Chemokines
Tokyo
Ophthalmology
Hematoxylin
Eosine Yellowish-(YS)
Ambulatory Surgical Procedures
Microscopy
Tooth
Japan
Phosphates
Staining and Labeling
Population

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Detection of neutrophils and possible involvement of interleukin-8 in diffuse lamellar keratitis after laser in situ keratomileusis. / Asano-Kato, Naoko; Toda, Ikuko; Shimmura, Shigeto; Noda-Tsuruya, Tomoko; Fukagawa, Kazumi; Yoshinaga, Masaru; Matsukawa, Akihiro; Tsubota, Kazuo.

In: Journal of Cataract and Refractive Surgery, Vol. 29, No. 10, 10.2003, p. 1996-2000.

Research output: Contribution to journalArticle

Asano-Kato, Naoko ; Toda, Ikuko ; Shimmura, Shigeto ; Noda-Tsuruya, Tomoko ; Fukagawa, Kazumi ; Yoshinaga, Masaru ; Matsukawa, Akihiro ; Tsubota, Kazuo. / Detection of neutrophils and possible involvement of interleukin-8 in diffuse lamellar keratitis after laser in situ keratomileusis. In: Journal of Cataract and Refractive Surgery. 2003 ; Vol. 29, No. 10. pp. 1996-2000.
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N2 - Purpose: To investigate the cell populations in diffuse lamellar keratitis (DLK) infiltration after laser in situ keratomileusis and the possible mechanism underlying the infiltration. Setting: Department of Ophthalmology, Tokyo Dental College, Chiba, Japan. Methods: To develop DLK in rabbit eyes, 25 μL of lipopolysaccharide (LPS) solution at a concentration of 50 μg/mL was applied to the stromal bed beneath corneal flaps. For control rabbits, phosphate-buffered saline was applied. Postoperative examination by slitlamp microscopy was performed for 3 days after surgery. Rabbit eyes were excised and examined for histopathology with hematoxylin and eosin staining. Immunohistochemical analysis for interleukin (IL)-8 was performed. Results: Diffuse lamellar keratitis-like inflammation composed mainly of neutrophils was reproduced by LPS instillation in rabbit eyes. In eyes with severe inflammation, IL-8 immunoreactivity was found in the stromal keratocytes and infiltrating neutrophils. Conclusions: The major cell type in the DLK infiltration induced by LPS instillation in rabbit eyes was the neutrophil. Interleukin-8, a prototype of CXC chemokine produced by keratocytes and neutrophils, may contribute to the development of DLK.

AB - Purpose: To investigate the cell populations in diffuse lamellar keratitis (DLK) infiltration after laser in situ keratomileusis and the possible mechanism underlying the infiltration. Setting: Department of Ophthalmology, Tokyo Dental College, Chiba, Japan. Methods: To develop DLK in rabbit eyes, 25 μL of lipopolysaccharide (LPS) solution at a concentration of 50 μg/mL was applied to the stromal bed beneath corneal flaps. For control rabbits, phosphate-buffered saline was applied. Postoperative examination by slitlamp microscopy was performed for 3 days after surgery. Rabbit eyes were excised and examined for histopathology with hematoxylin and eosin staining. Immunohistochemical analysis for interleukin (IL)-8 was performed. Results: Diffuse lamellar keratitis-like inflammation composed mainly of neutrophils was reproduced by LPS instillation in rabbit eyes. In eyes with severe inflammation, IL-8 immunoreactivity was found in the stromal keratocytes and infiltrating neutrophils. Conclusions: The major cell type in the DLK infiltration induced by LPS instillation in rabbit eyes was the neutrophil. Interleukin-8, a prototype of CXC chemokine produced by keratocytes and neutrophils, may contribute to the development of DLK.

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