Detection of micrococcus Luteus biofilm formation in microfluidic environments by pH measurement using an ion-sensitive field-effect transistor

Koji Matsuura, Yuka Asano, Akira Yamada, Keiji Naruse

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Biofilm formation in microfluidic channels is difficult to detect because sampling volumes are too small for conventional turbidity measurements. To detect biofilm formation, we used an ion-sensitive field-effect transistor (ISFET) measurement system to measure pH changes in small volumes of bacterial suspension. Cells of Micrococcus luteus (M. luteus) were cultured in polystyrene (PS) microtubes and polymethylmethacrylate (PMMA)-based microfluidic channels laminated with polyvinylidene chloride. In microtubes, concentrations of bacteria and pH in the suspension were analyzed by measuring turbidity and using an ISFET sensor, respectively. In microfluidic channels containing 20 μL of bacterial suspension, we measured pH changes using the ISFET sensor and monitored biofilm formation using a microscope. We detected acidification and alkalinization phases of M. luteus from the ISFET sensor signals in both microtubes and microfluidic channels. In the alkalinization phase, after 2 day culture, dense biofilm formation was observed at the bottom of the microfluidic channels. In this study, we used an ISFET sensor to detect Biofilm formation in clinical and industrial microfluidic environments by detecting alkalinization of the culture medium.

Original languageEnglish
Pages (from-to)2484-2493
Number of pages10
JournalSensors (Switzerland)
Volume13
Issue number2
DOIs
Publication statusPublished - 2013

Keywords

  • Alkalinization
  • Ion-sensing field-effect transistor
  • Micrococcus luteus biofilm
  • Microfluidic channel

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Electrical and Electronic Engineering

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