Detection of Legionella spp. in cooling tower water by the polymerase chain reaction method

M. Koide, A. Saito, N. Kusano, F. Higa

Research output: Contribution to journalComment/debate

60 Citations (Scopus)

Abstract

The presence of Legionella spp. in cooling tower water was investigated by using the polymerase chain reaction. Total Legionella spp. detection was performed with 20-mer 5S rRNA complementary DNA sequence primers, and specific Legionella pneumophila detection was performed with 20-mer and then 21-mer macrophage infectivity potentiator gene sequence primers. Of 27 cooling tower water samples, 25 were positive for Legionella spp., and 14 of these contained L. pneumophila.

Original languageEnglish
Pages (from-to)1943-1946
Number of pages4
JournalApplied and environmental microbiology
Volume59
Issue number6
DOIs
Publication statusPublished - Jan 1 1993
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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