Detection of Legionella spp. in cooling tower water by the polymerase chain reaction method

M. Koide, A. Saito, Nobuchika Kusano, F. Higa

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The presence of Legionella spp. in cooling tower water was investigated by using the polymerase chain reaction. Total Legionella spp. detection was performed with 20-mer 5S rRNA complementary DNA sequence primers, and specific Legionella pneumophila detection was performed with 20-mer and then 21-mer macrophage infectivity potentiator gene sequence primers. Of 27 cooling tower water samples, 25 were positive for Legionella spp., and 14 of these contained L. pneumophila.

Original languageEnglish
Pages (from-to)1943-1946
Number of pages4
JournalApplied and Environmental Microbiology
Volume59
Issue number6
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Legionella
Legionella pneumophila
polymerase chain reaction
cooling
Polymerase Chain Reaction
Water
infectivity
nucleotide sequences
complementary DNA
DNA Primers
water
DNA
gene
macrophages
pathogenicity
Complementary DNA
methodology
Macrophages
ribosomal RNA
Genes

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science(all)
  • Microbiology

Cite this

Detection of Legionella spp. in cooling tower water by the polymerase chain reaction method. / Koide, M.; Saito, A.; Kusano, Nobuchika; Higa, F.

In: Applied and Environmental Microbiology, Vol. 59, No. 6, 1993, p. 1943-1946.

Research output: Contribution to journalArticle

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