Abstract
We evaluated the usefulness of a Nested PCR method for detecting Legionella pneumophila. This method resulted in L. pneumophila specific detection as far as we evaluated. The first and second step PCR achieved the sensitivity as small as 10 pg and 10 fg of the target DNA, respectively. In the detection from Legionella seeded sputa, the method could detect 0.1 cfu/ml of the bacteria, and it took about 12 hours to detect the target DNA. We demonstrated that the Nested PCR method was superior in sensitivity and rapidity for isolation of the bacteria to the conventional using low pH treatment and selective media for Legionella.
| Original language | English |
|---|---|
| Pages (from-to) | 1084-1089 |
| Number of pages | 6 |
| Journal | Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases |
| Volume | 66 |
| Issue number | 8 |
| Publication status | Published - Aug 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine(all)
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Detection of Legionella pneumophila using a nested polymerase chain reaction. / Higa, F.; Koide, M.; Shinzato, T.; Nakamoto, A.; Miyara, T.; Gaja, M.; Tateyama, M.; Owan, I.; Kusano, Nobuchika; Kitsukawa, K.
In: Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases, Vol. 66, No. 8, 08.1992, p. 1084-1089.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of Legionella pneumophila using a nested polymerase chain reaction
AU - Higa, F.
AU - Koide, M.
AU - Shinzato, T.
AU - Nakamoto, A.
AU - Miyara, T.
AU - Gaja, M.
AU - Tateyama, M.
AU - Owan, I.
AU - Kusano, Nobuchika
AU - Kitsukawa, K.
PY - 1992/8
Y1 - 1992/8
N2 - We evaluated the usefulness of a Nested PCR method for detecting Legionella pneumophila. This method resulted in L. pneumophila specific detection as far as we evaluated. The first and second step PCR achieved the sensitivity as small as 10 pg and 10 fg of the target DNA, respectively. In the detection from Legionella seeded sputa, the method could detect 0.1 cfu/ml of the bacteria, and it took about 12 hours to detect the target DNA. We demonstrated that the Nested PCR method was superior in sensitivity and rapidity for isolation of the bacteria to the conventional using low pH treatment and selective media for Legionella.
AB - We evaluated the usefulness of a Nested PCR method for detecting Legionella pneumophila. This method resulted in L. pneumophila specific detection as far as we evaluated. The first and second step PCR achieved the sensitivity as small as 10 pg and 10 fg of the target DNA, respectively. In the detection from Legionella seeded sputa, the method could detect 0.1 cfu/ml of the bacteria, and it took about 12 hours to detect the target DNA. We demonstrated that the Nested PCR method was superior in sensitivity and rapidity for isolation of the bacteria to the conventional using low pH treatment and selective media for Legionella.
UR - http://www.scopus.com/inward/record.url?scp=0026903758&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026903758&partnerID=8YFLogxK
M3 - Article
C2 - 1402113
AN - SCOPUS:0026903758
VL - 66
SP - 1084
EP - 1089
JO - Nippon Densenbyo Gakkai zasshi
JF - Nippon Densenbyo Gakkai zasshi
SN - 0387-5911
IS - 8
ER -