Detection of Legionella pneumophila using a nested polymerase chain reaction

F. Higa, M. Koide, T. Shinzato, A. Nakamoto, T. Miyara, M. Gaja, M. Tateyama, I. Owan, Nobuchika Kusano, K. Kitsukawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We evaluated the usefulness of a Nested PCR method for detecting Legionella pneumophila. This method resulted in L. pneumophila specific detection as far as we evaluated. The first and second step PCR achieved the sensitivity as small as 10 pg and 10 fg of the target DNA, respectively. In the detection from Legionella seeded sputa, the method could detect 0.1 cfu/ml of the bacteria, and it took about 12 hours to detect the target DNA. We demonstrated that the Nested PCR method was superior in sensitivity and rapidity for isolation of the bacteria to the conventional using low pH treatment and selective media for Legionella.

Original languageEnglish
Pages (from-to)1084-1089
Number of pages6
JournalKansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases
Volume66
Issue number8
Publication statusPublished - Aug 1992
Externally publishedYes

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Legionella pneumophila
Legionella
Polymerase Chain Reaction
Bacteria
DNA
Sputum

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Higa, F., Koide, M., Shinzato, T., Nakamoto, A., Miyara, T., Gaja, M., ... Kitsukawa, K. (1992). Detection of Legionella pneumophila using a nested polymerase chain reaction. Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases, 66(8), 1084-1089.

Detection of Legionella pneumophila using a nested polymerase chain reaction. / Higa, F.; Koide, M.; Shinzato, T.; Nakamoto, A.; Miyara, T.; Gaja, M.; Tateyama, M.; Owan, I.; Kusano, Nobuchika; Kitsukawa, K.

In: Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases, Vol. 66, No. 8, 08.1992, p. 1084-1089.

Research output: Contribution to journalArticle

Higa, F, Koide, M, Shinzato, T, Nakamoto, A, Miyara, T, Gaja, M, Tateyama, M, Owan, I, Kusano, N & Kitsukawa, K 1992, 'Detection of Legionella pneumophila using a nested polymerase chain reaction', Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases, vol. 66, no. 8, pp. 1084-1089.
Higa, F. ; Koide, M. ; Shinzato, T. ; Nakamoto, A. ; Miyara, T. ; Gaja, M. ; Tateyama, M. ; Owan, I. ; Kusano, Nobuchika ; Kitsukawa, K. / Detection of Legionella pneumophila using a nested polymerase chain reaction. In: Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases. 1992 ; Vol. 66, No. 8. pp. 1084-1089.
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