Detection of landiolol using high-performance liquid chromatography/fluorescence: A blood esterase-sensitive ultra-short-acting β1-receptor antagonist

Manabu Suno, Takayuki Kunisawa, Akio Yamagishi, Takashi Ono, Joe Yamamoto, Takehiro Yamada, Yoshikazu Tasaki, Keiko Shimizu, Hiroshi Iwasaki, Kazuo Matsubara

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Landiolol hydrochloride, a new adrenergic β1-selective antagonist having an ultra-short half-life, is used to prevent tachyarrhythmia during surgery. Since landiolol is thought to be rapidly hydrolyzed to an inactivate metabolite by esterases, quantification of the drug concentration in the blood is impractical. The landiolol concentration in blood was halved within 5 min after blood sampling. This degradation was effectively prevented by pre-treatment with neostigmine (100 μg) in the sampling tube, but not by EDTA pre-treatment, indicating that landiolol could be metabolized by pseudocholinesterase in plasma. After the one-step solid-phase extraction, fluorescence detection of landiolol reduced chromatographic background signals and then improved assay sensitivity to the lower limit of 10 ng/ml in blood; this reproducible approach yielded coefficient variation of less than 6%. The blood concentration-time profile of landiolol hydrochloride in patients of the present investigation afforded more practical assessment than previously reported studies, thus improving accuracy and facilitating detailed pharmacokinetic study in relation to the pharmacological action of drug.

Original languageEnglish
Pages (from-to)1705-1708
Number of pages4
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume877
Issue number16-17
DOIs
Publication statusPublished - Jun 1 2009
Externally publishedYes

Keywords

  • HPLC/fluorescence
  • Landiolol
  • One-step solid-phase extraction
  • Pseudocholinesterase
  • Ultra-short-acting β-receptor antagonist

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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