Detection of interfacial phenomena with osteoblast-like cell adhesion on hydroxyapatite and oxidized polystyrene by the quartz crystal microbalance with dissipation

Motohiro Tagaya, Toshiyuki Ikoma, Taro Takemura, Nobutaka Hanagata, Mitsuhiro Okuda, Tomohiko Yoshioka, Junzo Tanaka

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The adhesion process of osteoblast-like cells on hydroxyapatite (HAp) and oxidized polystyrene (PSox) was investigated using a quartz crystal microbalance with dissipation (QCM-D), confocal laser scanning microscope (CLSM), and atomic force microscope (AFM) techniques in order to clarify the interfacial phenomena between the surfaces and cells. The interfacial viscoelastic properties (shear viscosity (ηad), elastic shear modulus (μad), and tan δ) of the preadsorbed protein layer and the interface layer between the surfaces and cells were estimated using a Voigt-based viscoelastic model from the measured frequency (Δf) and dissipation shift (ΔD) curves. In the ΔD-Δf plots, the cell adhesion process on HAp was classified as (1) a mass increase only, (2) increases in both mass and ΔD, and (3) slight decreases in mass and ΔD. On PSox, only ΔD increases were observed, indicating that the adhesion behavior depended on the surface properties. The interfacial μad value between the material surfaces and cells increased with the number of adherent cells, whereas ηad and tanδ decreased slightly, irrespective of the surface. Thus, the interfacial layer changed the elasticity to viscosity with an increase in the number. The tan δ values on HAp were higher than those on PSox and exceeded 1.0. Furthermore, the pseudopod-like structures of the cells on HAp had periodic stripe patterns stained with a type I collagen antibody, whereas those on PSox had cell-membrane-like structures unstained with type I collagen. These results indicate that the interfacial layers on PSox and HAp exhibit elasticity and viscosity, respectively, indicating that the rearrangements of the extracellular matrix and cytoskeleton changes cause different cell-surface interactions. Therefore, the different cell adhesion process, interfacial viscoelasticity, and morphology depending on the surfaces were successfully monitored in situ and evaluated by the QCM-D technique combined with other techniques.

Original languageEnglish
Pages (from-to)7635-7644
Number of pages10
JournalLangmuir
Volume27
Issue number12
DOIs
Publication statusPublished - Jun 21 2011
Externally publishedYes

Fingerprint

osteoblasts
Quartz crystal microbalances
Polystyrenes
Osteoblasts
Cell adhesion
Durapatite
quartz crystals
Hydroxyapatite
microbalances
polystyrene
adhesion
dissipation
cells
Collagen Type I
Collagen
Elasticity
Microscopes
Adhesion
Viscosity
collagens

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Detection of interfacial phenomena with osteoblast-like cell adhesion on hydroxyapatite and oxidized polystyrene by the quartz crystal microbalance with dissipation. / Tagaya, Motohiro; Ikoma, Toshiyuki; Takemura, Taro; Hanagata, Nobutaka; Okuda, Mitsuhiro; Yoshioka, Tomohiko; Tanaka, Junzo.

In: Langmuir, Vol. 27, No. 12, 21.06.2011, p. 7635-7644.

Research output: Contribution to journalArticle

Tagaya, Motohiro ; Ikoma, Toshiyuki ; Takemura, Taro ; Hanagata, Nobutaka ; Okuda, Mitsuhiro ; Yoshioka, Tomohiko ; Tanaka, Junzo. / Detection of interfacial phenomena with osteoblast-like cell adhesion on hydroxyapatite and oxidized polystyrene by the quartz crystal microbalance with dissipation. In: Langmuir. 2011 ; Vol. 27, No. 12. pp. 7635-7644.
@article{6bd58894475d4d109cabbdaaf6fe6992,
title = "Detection of interfacial phenomena with osteoblast-like cell adhesion on hydroxyapatite and oxidized polystyrene by the quartz crystal microbalance with dissipation",
abstract = "The adhesion process of osteoblast-like cells on hydroxyapatite (HAp) and oxidized polystyrene (PSox) was investigated using a quartz crystal microbalance with dissipation (QCM-D), confocal laser scanning microscope (CLSM), and atomic force microscope (AFM) techniques in order to clarify the interfacial phenomena between the surfaces and cells. The interfacial viscoelastic properties (shear viscosity (ηad), elastic shear modulus (μad), and tan δ) of the preadsorbed protein layer and the interface layer between the surfaces and cells were estimated using a Voigt-based viscoelastic model from the measured frequency (Δf) and dissipation shift (ΔD) curves. In the ΔD-Δf plots, the cell adhesion process on HAp was classified as (1) a mass increase only, (2) increases in both mass and ΔD, and (3) slight decreases in mass and ΔD. On PSox, only ΔD increases were observed, indicating that the adhesion behavior depended on the surface properties. The interfacial μad value between the material surfaces and cells increased with the number of adherent cells, whereas ηad and tanδ decreased slightly, irrespective of the surface. Thus, the interfacial layer changed the elasticity to viscosity with an increase in the number. The tan δ values on HAp were higher than those on PSox and exceeded 1.0. Furthermore, the pseudopod-like structures of the cells on HAp had periodic stripe patterns stained with a type I collagen antibody, whereas those on PSox had cell-membrane-like structures unstained with type I collagen. These results indicate that the interfacial layers on PSox and HAp exhibit elasticity and viscosity, respectively, indicating that the rearrangements of the extracellular matrix and cytoskeleton changes cause different cell-surface interactions. Therefore, the different cell adhesion process, interfacial viscoelasticity, and morphology depending on the surfaces were successfully monitored in situ and evaluated by the QCM-D technique combined with other techniques.",
author = "Motohiro Tagaya and Toshiyuki Ikoma and Taro Takemura and Nobutaka Hanagata and Mitsuhiro Okuda and Tomohiko Yoshioka and Junzo Tanaka",
year = "2011",
month = "6",
day = "21",
doi = "10.1021/la200008z",
language = "English",
volume = "27",
pages = "7635--7644",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "12",

}

TY - JOUR

T1 - Detection of interfacial phenomena with osteoblast-like cell adhesion on hydroxyapatite and oxidized polystyrene by the quartz crystal microbalance with dissipation

AU - Tagaya, Motohiro

AU - Ikoma, Toshiyuki

AU - Takemura, Taro

AU - Hanagata, Nobutaka

AU - Okuda, Mitsuhiro

AU - Yoshioka, Tomohiko

AU - Tanaka, Junzo

PY - 2011/6/21

Y1 - 2011/6/21

N2 - The adhesion process of osteoblast-like cells on hydroxyapatite (HAp) and oxidized polystyrene (PSox) was investigated using a quartz crystal microbalance with dissipation (QCM-D), confocal laser scanning microscope (CLSM), and atomic force microscope (AFM) techniques in order to clarify the interfacial phenomena between the surfaces and cells. The interfacial viscoelastic properties (shear viscosity (ηad), elastic shear modulus (μad), and tan δ) of the preadsorbed protein layer and the interface layer between the surfaces and cells were estimated using a Voigt-based viscoelastic model from the measured frequency (Δf) and dissipation shift (ΔD) curves. In the ΔD-Δf plots, the cell adhesion process on HAp was classified as (1) a mass increase only, (2) increases in both mass and ΔD, and (3) slight decreases in mass and ΔD. On PSox, only ΔD increases were observed, indicating that the adhesion behavior depended on the surface properties. The interfacial μad value between the material surfaces and cells increased with the number of adherent cells, whereas ηad and tanδ decreased slightly, irrespective of the surface. Thus, the interfacial layer changed the elasticity to viscosity with an increase in the number. The tan δ values on HAp were higher than those on PSox and exceeded 1.0. Furthermore, the pseudopod-like structures of the cells on HAp had periodic stripe patterns stained with a type I collagen antibody, whereas those on PSox had cell-membrane-like structures unstained with type I collagen. These results indicate that the interfacial layers on PSox and HAp exhibit elasticity and viscosity, respectively, indicating that the rearrangements of the extracellular matrix and cytoskeleton changes cause different cell-surface interactions. Therefore, the different cell adhesion process, interfacial viscoelasticity, and morphology depending on the surfaces were successfully monitored in situ and evaluated by the QCM-D technique combined with other techniques.

AB - The adhesion process of osteoblast-like cells on hydroxyapatite (HAp) and oxidized polystyrene (PSox) was investigated using a quartz crystal microbalance with dissipation (QCM-D), confocal laser scanning microscope (CLSM), and atomic force microscope (AFM) techniques in order to clarify the interfacial phenomena between the surfaces and cells. The interfacial viscoelastic properties (shear viscosity (ηad), elastic shear modulus (μad), and tan δ) of the preadsorbed protein layer and the interface layer between the surfaces and cells were estimated using a Voigt-based viscoelastic model from the measured frequency (Δf) and dissipation shift (ΔD) curves. In the ΔD-Δf plots, the cell adhesion process on HAp was classified as (1) a mass increase only, (2) increases in both mass and ΔD, and (3) slight decreases in mass and ΔD. On PSox, only ΔD increases were observed, indicating that the adhesion behavior depended on the surface properties. The interfacial μad value between the material surfaces and cells increased with the number of adherent cells, whereas ηad and tanδ decreased slightly, irrespective of the surface. Thus, the interfacial layer changed the elasticity to viscosity with an increase in the number. The tan δ values on HAp were higher than those on PSox and exceeded 1.0. Furthermore, the pseudopod-like structures of the cells on HAp had periodic stripe patterns stained with a type I collagen antibody, whereas those on PSox had cell-membrane-like structures unstained with type I collagen. These results indicate that the interfacial layers on PSox and HAp exhibit elasticity and viscosity, respectively, indicating that the rearrangements of the extracellular matrix and cytoskeleton changes cause different cell-surface interactions. Therefore, the different cell adhesion process, interfacial viscoelasticity, and morphology depending on the surfaces were successfully monitored in situ and evaluated by the QCM-D technique combined with other techniques.

UR - http://www.scopus.com/inward/record.url?scp=79959202080&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959202080&partnerID=8YFLogxK

U2 - 10.1021/la200008z

DO - 10.1021/la200008z

M3 - Article

VL - 27

SP - 7635

EP - 7644

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 12

ER -