Detection of identical isolates of Enterococcus faecalis from the blood and oral mucosa in a patient with infective endocarditis

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3 Citations (Scopus)

Abstract

The detection of infective endocarditis (IE) of oral origin has been previously discussed. However, there are few reports confirming this infection using molecular biological techniques. We herein describe the case of a 67-year-old man who developed IE. Blood culture samples and strains obtained from the gingival and buccal mucosa showed 100% identity to Enterococcus faecalis JCM 5803 on sequencing of 16S rRNA gene fragments. A random amplification of polymorphic DNA (RAPD) analysis showed the same pattern for these samples, thus confirming the identity of E. faecalis isolates in the blood and oral mucosa. Our observations provide novel information regarding the level of identity between IE pathogens and oral bacteria.

Original languageEnglish
Pages (from-to)1809-1814
Number of pages6
JournalInternal Medicine
Volume54
Issue number14
DOIs
Publication statusPublished - Jul 15 2015

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Enterococcus faecalis
Mouth Mucosa
Endocarditis
rRNA Genes
Bacteria
DNA
Infection

Keywords

  • Causative pathogen
  • Identity
  • Infective endocarditis
  • Oral bacteria

ASJC Scopus subject areas

  • Internal Medicine

Cite this

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title = "Detection of identical isolates of Enterococcus faecalis from the blood and oral mucosa in a patient with infective endocarditis",
abstract = "The detection of infective endocarditis (IE) of oral origin has been previously discussed. However, there are few reports confirming this infection using molecular biological techniques. We herein describe the case of a 67-year-old man who developed IE. Blood culture samples and strains obtained from the gingival and buccal mucosa showed 100{\%} identity to Enterococcus faecalis JCM 5803 on sequencing of 16S rRNA gene fragments. A random amplification of polymorphic DNA (RAPD) analysis showed the same pattern for these samples, thus confirming the identity of E. faecalis isolates in the blood and oral mucosa. Our observations provide novel information regarding the level of identity between IE pathogens and oral bacteria.",
keywords = "Causative pathogen, Identity, Infective endocarditis, Oral bacteria",
author = "Akemi Okui and Yoshihiko Soga and Susumu Kokeguchi and Motoko Nose and Reiko Yamanaka and Nobuchika Kusano and Manabu Morita",
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T1 - Detection of identical isolates of Enterococcus faecalis from the blood and oral mucosa in a patient with infective endocarditis

AU - Okui, Akemi

AU - Soga, Yoshihiko

AU - Kokeguchi, Susumu

AU - Nose, Motoko

AU - Yamanaka, Reiko

AU - Kusano, Nobuchika

AU - Morita, Manabu

PY - 2015/7/15

Y1 - 2015/7/15

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AB - The detection of infective endocarditis (IE) of oral origin has been previously discussed. However, there are few reports confirming this infection using molecular biological techniques. We herein describe the case of a 67-year-old man who developed IE. Blood culture samples and strains obtained from the gingival and buccal mucosa showed 100% identity to Enterococcus faecalis JCM 5803 on sequencing of 16S rRNA gene fragments. A random amplification of polymorphic DNA (RAPD) analysis showed the same pattern for these samples, thus confirming the identity of E. faecalis isolates in the blood and oral mucosa. Our observations provide novel information regarding the level of identity between IE pathogens and oral bacteria.

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