Detection of human papillomavirus genome and analysis of expression of c-myc and Ha-ras oncogenes in invasive cervical carcinomas

Tsuyoshi Iwasaka, Masatoshi Yokoyama, Mamoru Oouchida, Norito Matsuo, Kouichi Hara, Kouzou Fukuyama, Toru Hachisuga, Kouichi Fukuda, Hajime Sugimori

Research output: Contribution to journalArticle

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Abstract

Invasive carcinomas of the uterine cervix of 38 patients were examined for the presence of human papillomavirus (HPV) genomes and for the state of the c-myc and Ha-ras oncogenes. A combination of Southern blot hybridization and polymerase chain reaction revealed the presence of the genome of HPV type 16 in 17 tumors (45%), that of HPV type 18 in 3 tumors (8%), and that of unknown types in 16 others (42%), while no viral DNA sequences were detected in 2 tumors. Of the 38 tumors, c-myc amplification was found in only 1 tumor, while there was no Ha-ras amplification. Overexpression of the c-myc gene was observed in 15 (44%) of the 34 tumors analyzed, while there was no overexpression of Ha-ras. Of the 23 squamous cell carcinomas analyzed, relapse-free rates at 24 months were 55% in tumors with c-myc overexpression and 100% in case of tumors with no c-myc overexpression, respectively. The results suggest the possibility that activation of the c-myc oncogene is involved in tumor progression.

Original languageEnglish
Pages (from-to)298-303
Number of pages6
JournalGynecologic Oncology
Volume46
Issue number3
DOIs
Publication statusPublished - 1992
Externally publishedYes

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ras Genes
Human Genome
Carcinoma
Neoplasms
myc Genes
Human papillomavirus 18
Human papillomavirus 16
Viral DNA
Southern Blotting
Cervix Uteri
Squamous Cell Carcinoma
Genome
Recurrence
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Oncology

Cite this

Detection of human papillomavirus genome and analysis of expression of c-myc and Ha-ras oncogenes in invasive cervical carcinomas. / Iwasaka, Tsuyoshi; Yokoyama, Masatoshi; Oouchida, Mamoru; Matsuo, Norito; Hara, Kouichi; Fukuyama, Kouzou; Hachisuga, Toru; Fukuda, Kouichi; Sugimori, Hajime.

In: Gynecologic Oncology, Vol. 46, No. 3, 1992, p. 298-303.

Research output: Contribution to journalArticle

Iwasaka, Tsuyoshi ; Yokoyama, Masatoshi ; Oouchida, Mamoru ; Matsuo, Norito ; Hara, Kouichi ; Fukuyama, Kouzou ; Hachisuga, Toru ; Fukuda, Kouichi ; Sugimori, Hajime. / Detection of human papillomavirus genome and analysis of expression of c-myc and Ha-ras oncogenes in invasive cervical carcinomas. In: Gynecologic Oncology. 1992 ; Vol. 46, No. 3. pp. 298-303.
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AU - Matsuo, Norito

AU - Hara, Kouichi

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AU - Fukuda, Kouichi

AU - Sugimori, Hajime

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