Detection of human papillomavirus genome and analysis of expression of c-myc and Ha-ras oncogenes in invasive cervical carcinomas

Tsuyoshi Iwasaka, Masatoshi Yokoyama, Mamoru Oh-uchida, Norito Matsuo, Kouichi Hara, Kouzou Fukuyama, Toru Hachisuga, Kouichi Fukuda, Hajime Sugimori

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28 Citations (Scopus)

Abstract

Invasive carcinomas of the uterine cervix of 38 patients were examined for the presence of human papillomavirus (HPV) genomes and for the state of the c-myc and Ha-ras oncogenes. A combination of Southern blot hybridization and polymerase chain reaction revealed the presence of the genome of HPV type 16 in 17 tumors (45%), that of HPV type 18 in 3 tumors (8%), and that of unknown types in 16 others (42%), while no viral DNA sequences were detected in 2 tumors. Of the 38 tumors, c-myc amplification was found in only 1 tumor, while there was no Ha-ras amplification. Overexpression of the c-myc gene was observed in 15 (44%) of the 34 tumors analyzed, while there was no overexpression of Ha-ras. Of the 23 squamous cell carcinomas analyzed, relapse-free rates at 24 months were 55% in tumors with c-myc overexpression and 100% in case of tumors with no c-myc overexpression, respectively. The results suggest the possibility that activation of the c-myc oncogene is involved in tumor progression.

Original languageEnglish
Pages (from-to)298-303
Number of pages6
JournalGynecologic Oncology
Volume46
Issue number3
DOIs
Publication statusPublished - Sep 1992
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynaecology

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