Detection of herpes simplex and varicella-zoster virus DNA by field-inversion gel electrophoresis from clinical materials

Y. Arao, M. Yoshida, T. Sata, A. Nakatsukasa, K. Miyoshi, M. Yamada, F. Uno, T. Kurata, S. Nii

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A simple method using field-inversion gel electrophoresis (FIGE) was applied to detect herpes simplex virus (HSV) and varicella-zoster virus (VZV) genomes in clinical specimens. The whole genomes of these viruses could be detected in small vesicle tissues by the FIGE method regardless of their clinical stages of skin lesions. And the sensitivity of the FIGE method was equivalent to that of an immunofluorescent (IF) method. These data indicated usefulness of the FIGE method to detect the whole genomes of HSV and VZV in clinical specimens.

Original languageEnglish
Pages (from-to)1217-1221
Number of pages5
JournalMicrobiology and Immunology
Volume36
Issue number11
Publication statusPublished - 1992

Fingerprint

Herpes Simplex
Human Herpesvirus 3
Pulsed Field Gel Electrophoresis
DNA
Genome
Simplexvirus
Viruses
Skin

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology
  • Microbiology (medical)

Cite this

Detection of herpes simplex and varicella-zoster virus DNA by field-inversion gel electrophoresis from clinical materials. / Arao, Y.; Yoshida, M.; Sata, T.; Nakatsukasa, A.; Miyoshi, K.; Yamada, M.; Uno, F.; Kurata, T.; Nii, S.

In: Microbiology and Immunology, Vol. 36, No. 11, 1992, p. 1217-1221.

Research output: Contribution to journalArticle

Arao, Y, Yoshida, M, Sata, T, Nakatsukasa, A, Miyoshi, K, Yamada, M, Uno, F, Kurata, T & Nii, S 1992, 'Detection of herpes simplex and varicella-zoster virus DNA by field-inversion gel electrophoresis from clinical materials', Microbiology and Immunology, vol. 36, no. 11, pp. 1217-1221.
Arao, Y. ; Yoshida, M. ; Sata, T. ; Nakatsukasa, A. ; Miyoshi, K. ; Yamada, M. ; Uno, F. ; Kurata, T. ; Nii, S. / Detection of herpes simplex and varicella-zoster virus DNA by field-inversion gel electrophoresis from clinical materials. In: Microbiology and Immunology. 1992 ; Vol. 36, No. 11. pp. 1217-1221.
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AU - Uno, F.

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