Abstract
Materials and Methods: We examined HCV-RNA immunoprecipitated with anti-human IgG, A and M antibodies by reverse transcription-polymerase chain reaction. Results: In thirty-nine (91%) of 43 patients, composed of 35 chronic hepatitis (CH) and 8 liver cirrhosis (LC), HCV-RNA was detected in the CIC. All 43 patients analyzed were classified into the following three categories; HCV-RNA was detected only in the supernatant (S pattern, 4 patients), both in the supernatant and the precipitate (SP pattern, 27 patients), and only in the precipitate (P pattern, 12 patients). SP pattern was most common in chronic HCV infection, and the frequency of SP pattern decreased with the progression of liver disease. P pattern was significantly more frequent in patients with higher γ-globulin, levels, histologically indicated LC, and antibody to HCV envelope protein. Conclusion: We found that HCV formed a CIC in most patients with chronic HCV infection, and that the formation of CIC might be related to the stage of chronic HCV infection.
| Original language | English |
|---|---|
| Pages (from-to) | 582-585 |
| Number of pages | 4 |
| Journal | Hepato-Gastroenterology |
| Volume | 43 |
| Issue number | 9 |
| Publication status | Published - 1996 |
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Keywords
- Circulating immune complex
- Hepatitis C virus
- RNA
- RT-PCR
ASJC Scopus subject areas
- Gastroenterology
Cite this
Detection of hepatitis C virus RNA in circulating immune complexes by RT-PCR. / Morita, Takechiyo; Hada, Hajime; Koide, Norio; Shiraha, Hidenori; Shinji, Toshiyuki; Nakamura, Masaki; Ujike, Kouzo; Wato, Masaki; Shimomura, Hiroyuki; Tsuji, Takao.
In: Hepato-Gastroenterology, Vol. 43, No. 9, 1996, p. 582-585.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of hepatitis C virus RNA in circulating immune complexes by RT-PCR
AU - Morita, Takechiyo
AU - Hada, Hajime
AU - Koide, Norio
AU - Shiraha, Hidenori
AU - Shinji, Toshiyuki
AU - Nakamura, Masaki
AU - Ujike, Kouzo
AU - Wato, Masaki
AU - Shimomura, Hiroyuki
AU - Tsuji, Takao
PY - 1996
Y1 - 1996
N2 - Materials and Methods: We examined HCV-RNA immunoprecipitated with anti-human IgG, A and M antibodies by reverse transcription-polymerase chain reaction. Results: In thirty-nine (91%) of 43 patients, composed of 35 chronic hepatitis (CH) and 8 liver cirrhosis (LC), HCV-RNA was detected in the CIC. All 43 patients analyzed were classified into the following three categories; HCV-RNA was detected only in the supernatant (S pattern, 4 patients), both in the supernatant and the precipitate (SP pattern, 27 patients), and only in the precipitate (P pattern, 12 patients). SP pattern was most common in chronic HCV infection, and the frequency of SP pattern decreased with the progression of liver disease. P pattern was significantly more frequent in patients with higher γ-globulin, levels, histologically indicated LC, and antibody to HCV envelope protein. Conclusion: We found that HCV formed a CIC in most patients with chronic HCV infection, and that the formation of CIC might be related to the stage of chronic HCV infection.
AB - Materials and Methods: We examined HCV-RNA immunoprecipitated with anti-human IgG, A and M antibodies by reverse transcription-polymerase chain reaction. Results: In thirty-nine (91%) of 43 patients, composed of 35 chronic hepatitis (CH) and 8 liver cirrhosis (LC), HCV-RNA was detected in the CIC. All 43 patients analyzed were classified into the following three categories; HCV-RNA was detected only in the supernatant (S pattern, 4 patients), both in the supernatant and the precipitate (SP pattern, 27 patients), and only in the precipitate (P pattern, 12 patients). SP pattern was most common in chronic HCV infection, and the frequency of SP pattern decreased with the progression of liver disease. P pattern was significantly more frequent in patients with higher γ-globulin, levels, histologically indicated LC, and antibody to HCV envelope protein. Conclusion: We found that HCV formed a CIC in most patients with chronic HCV infection, and that the formation of CIC might be related to the stage of chronic HCV infection.
KW - Circulating immune complex
KW - Hepatitis C virus
KW - RNA
KW - RT-PCR
UR - http://www.scopus.com/inward/record.url?scp=8944262041&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=8944262041&partnerID=8YFLogxK
M3 - Article
C2 - 8799399
AN - SCOPUS:8944262041
VL - 43
SP - 582
EP - 585
JO - Acta hepato-splenologica
JF - Acta hepato-splenologica
SN - 0172-6390
IS - 9
ER -