Detection of Hepatitis C Virus Infection by Enzyme‐linked Immunosorbent Assay System Using Core Protein Expressed in Escherichia coli

Kanae Muraiso; Makoto Hijikata; Nobuyuki Kato; Kunitada Shimotohno; Nobuo Okazaki; Showgo Ohkoshi; Masashi Uura; Shuichi Kaneko; Kenichi Kobayashi; Masao Omata; Hiroo Ohnishi; Yasutoshi Mutou; Nobu Hattori

doi:10.1111/j.1349-7006.1991.tb01914.x

Detection of Hepatitis C Virus Infection by Enzyme‐linked Immunosorbent Assay System Using Core Protein Expressed in Escherichia coli

Kanae Muraiso, Makoto Hijikata, Nobuyuki Kato, Kunitada Shimotohno, Nobuo Okazaki, Showgo Ohkoshi, Masashi Uura, Shuichi Kaneko, Kenichi Kobayashi, Masao Omata, Hiroo Ohnishi, Yasutoshi Mutou, Nobu Hattori

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Enzyme‐linked immunosorbent assay of the core protein of hepatitis C virus (HCV) expressed in E. coli led to detection of the antibody against this virus in patients with chronic hepatitis. Some of the negative results obtained using a different viral protein became positive with this E. coli‐expressed viral protein, and were also positive for the viral RNA. Thus, use of the core protein of HCV facilitates accurate detection of HCV infection.

Original language	English
Pages (from-to)	879-882
Number of pages	4
Journal	Japanese Journal of Cancer Research
Volume	82
Issue number	8
DOIs	https://doi.org/10.1111/j.1349-7006.1991.tb01914.x
Publication status	Published - Aug 1991

Keywords

ELISA
Hepatitis
Hepatitis C virus
Serodiagnosis

ASJC Scopus subject areas

Oncology
Cancer Research

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Muraiso, K., Hijikata, M., Kato, N., Shimotohno, K., Okazaki, N., Ohkoshi, S., Uura, M., Kaneko, S., Kobayashi, K., Omata, M., Ohnishi, H., Mutou, Y., & Hattori, N. (1991). Detection of Hepatitis C Virus Infection by Enzyme‐linked Immunosorbent Assay System Using Core Protein Expressed in Escherichia coli. Japanese Journal of Cancer Research, 82(8), 879-882. https://doi.org/10.1111/j.1349-7006.1991.tb01914.x

Detection of Hepatitis C Virus Infection by Enzyme‐linked Immunosorbent Assay System Using Core Protein Expressed in Escherichia coli. / Muraiso, Kanae; Hijikata, Makoto; Kato, Nobuyuki; Shimotohno, Kunitada; Okazaki, Nobuo; Ohkoshi, Showgo; Uura, Masashi; Kaneko, Shuichi; Kobayashi, Kenichi; Omata, Masao; Ohnishi, Hiroo; Mutou, Yasutoshi; Hattori, Nobu.

In: Japanese Journal of Cancer Research, Vol. 82, No. 8, 08.1991, p. 879-882.

Research output: Contribution to journal › Article › peer-review

Muraiso, K, Hijikata, M, Kato, N, Shimotohno, K, Okazaki, N, Ohkoshi, S, Uura, M, Kaneko, S, Kobayashi, K, Omata, M, Ohnishi, H, Mutou, Y & Hattori, N 1991, 'Detection of Hepatitis C Virus Infection by Enzyme‐linked Immunosorbent Assay System Using Core Protein Expressed in Escherichia coli', Japanese Journal of Cancer Research, vol. 82, no. 8, pp. 879-882. https://doi.org/10.1111/j.1349-7006.1991.tb01914.x

Muraiso, Kanae ; Hijikata, Makoto ; Kato, Nobuyuki ; Shimotohno, Kunitada ; Okazaki, Nobuo ; Ohkoshi, Showgo ; Uura, Masashi ; Kaneko, Shuichi ; Kobayashi, Kenichi ; Omata, Masao ; Ohnishi, Hiroo ; Mutou, Yasutoshi ; Hattori, Nobu. / Detection of Hepatitis C Virus Infection by Enzyme‐linked Immunosorbent Assay System Using Core Protein Expressed in Escherichia coli. In: Japanese Journal of Cancer Research. 1991 ; Vol. 82, No. 8. pp. 879-882.

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