Detection of Hepatitis C Virus Infection by Enzyme‐linked Immunosorbent Assay System Using Core Protein Expressed in Escherichia coli

Kanae Muraiso; Makoto Hijikata; Nobuyuki Kato; Kunitada Shimotohno; Nobuo Okazaki; Showgo Ohkoshi; Masashi Uura; Shuichi Kaneko; Kenichi Kobayashi; Masao Omata; Hiroo Ohnishi; Yasutoshi Mutou; Nobu Hattori

doi:10.1111/j.1349-7006.1991.tb01914.x

Detection of Hepatitis C Virus Infection by Enzyme‐linked Immunosorbent Assay System Using Core Protein Expressed in Escherichia coli

Kanae Muraiso, Makoto Hijikata, Nobuyuki Kato, Kunitada Shimotohno, Nobuo Okazaki, Showgo Ohkoshi, Masashi Uura, Shuichi Kaneko, Kenichi Kobayashi, Masao Omata, Hiroo Ohnishi, Yasutoshi Mutou, Nobu Hattori

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Enzyme‐linked immunosorbent assay of the core protein of hepatitis C virus (HCV) expressed in E. coli led to detection of the antibody against this virus in patients with chronic hepatitis. Some of the negative results obtained using a different viral protein became positive with this E. coli‐expressed viral protein, and were also positive for the viral RNA. Thus, use of the core protein of HCV facilitates accurate detection of HCV infection.

Original language	English
Pages (from-to)	879-882
Number of pages	4
Journal	Japanese Journal of Cancer Research
Volume	82
Issue number	8
DOIs	https://doi.org/10.1111/j.1349-7006.1991.tb01914.x
Publication status	Published - Aug 1991
Externally published	Yes

Keywords

ELISA
Hepatitis
Hepatitis C virus
Serodiagnosis

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1349-7006.1991.tb01914.x

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Muraiso, K., Hijikata, M., Kato, N., Shimotohno, K., Okazaki, N., Ohkoshi, S., Uura, M., Kaneko, S., Kobayashi, K., Omata, M., Ohnishi, H., Mutou, Y., & Hattori, N. (1991). Detection of Hepatitis C Virus Infection by Enzyme‐linked Immunosorbent Assay System Using Core Protein Expressed in Escherichia coli. Japanese Journal of Cancer Research, 82(8), 879-882. https://doi.org/10.1111/j.1349-7006.1991.tb01914.x

Muraiso, K, Hijikata, M, Kato, N, Shimotohno, K, Okazaki, N, Ohkoshi, S, Uura, M, Kaneko, S, Kobayashi, K, Omata, M, Ohnishi, H, Mutou, Y & Hattori, N 1991, 'Detection of Hepatitis C Virus Infection by Enzyme‐linked Immunosorbent Assay System Using Core Protein Expressed in Escherichia coli', Japanese Journal of Cancer Research, vol. 82, no. 8, pp. 879-882. https://doi.org/10.1111/j.1349-7006.1991.tb01914.x

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abstract = "Enzyme‐linked immunosorbent assay of the core protein of hepatitis C virus (HCV) expressed in E. coli led to detection of the antibody against this virus in patients with chronic hepatitis. Some of the negative results obtained using a different viral protein became positive with this E. coli‐expressed viral protein, and were also positive for the viral RNA. Thus, use of the core protein of HCV facilitates accurate detection of HCV infection.",

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author = "Kanae Muraiso and Makoto Hijikata and Nobuyuki Kato and Kunitada Shimotohno and Nobuo Okazaki and Showgo Ohkoshi and Masashi Uura and Shuichi Kaneko and Kenichi Kobayashi and Masao Omata and Hiroo Ohnishi and Yasutoshi Mutou and Nobu Hattori",

year = "1991",

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doi = "10.1111/j.1349-7006.1991.tb01914.x",

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T1 - Detection of Hepatitis C Virus Infection by Enzyme‐linked Immunosorbent Assay System Using Core Protein Expressed in Escherichia coli

AU - Muraiso, Kanae

AU - Hijikata, Makoto

AU - Kato, Nobuyuki

AU - Shimotohno, Kunitada

AU - Okazaki, Nobuo

AU - Ohkoshi, Showgo

AU - Uura, Masashi

AU - Kaneko, Shuichi

AU - Kobayashi, Kenichi

AU - Omata, Masao

AU - Ohnishi, Hiroo

AU - Mutou, Yasutoshi

AU - Hattori, Nobu

PY - 1991/8

Y1 - 1991/8

N2 - Enzyme‐linked immunosorbent assay of the core protein of hepatitis C virus (HCV) expressed in E. coli led to detection of the antibody against this virus in patients with chronic hepatitis. Some of the negative results obtained using a different viral protein became positive with this E. coli‐expressed viral protein, and were also positive for the viral RNA. Thus, use of the core protein of HCV facilitates accurate detection of HCV infection.

AB - Enzyme‐linked immunosorbent assay of the core protein of hepatitis C virus (HCV) expressed in E. coli led to detection of the antibody against this virus in patients with chronic hepatitis. Some of the negative results obtained using a different viral protein became positive with this E. coli‐expressed viral protein, and were also positive for the viral RNA. Thus, use of the core protein of HCV facilitates accurate detection of HCV infection.

KW - ELISA

KW - Hepatitis

KW - Hepatitis C virus

KW - Serodiagnosis

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Detection of Hepatitis C Virus Infection by Enzyme‐linked Immunosorbent Assay System Using Core Protein Expressed in Escherichia coli

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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