Detection of hepatitis C virus infection by enzyme-linked immunosorbent assay system using core protein expressed in Escherichia coli

Kanae Muraiso, Makoto Hijikata, Nobuyuki Kato, Kunitada Shimotohno, Nobuo Okazaki, Showgo Ohkoshi, Masashi Uura, Shuichi Kaneko, Kenichi Kobayashi, Masao Omata, Hiroo Ohnishi, Yasutoshi Mutou, Nobu Hattori

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Enzyme-linked immunosorbent assay of the core protein of hepatitis C virus (HCV) expressed in E. coli led to detection of the antibody against this virus in patients with chronic hepatitis. Some of the negative results obtained using a different viral protein became positive with this E. coli-expressed viral protein, and were also positive for the viral RNA. Thus, use of the core protein of HCV facilitates accurate detection of HCV infection.

Original languageEnglish
Pages (from-to)879-882
Number of pages4
JournalJapanese Journal of Cancer Research
Volume82
Issue number8
Publication statusPublished - Aug 1991
Externally publishedYes

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Viral Proteins
Virus Diseases
Hepacivirus
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Viral RNA
Chronic Hepatitis
Proteins
Viruses
Antibodies
Hepatitis C virus nucleocapsid protein

Keywords

  • ELISA
  • Hepatitis
  • Hepatitis C virus
  • Serodiagnosis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Detection of hepatitis C virus infection by enzyme-linked immunosorbent assay system using core protein expressed in Escherichia coli. / Muraiso, Kanae; Hijikata, Makoto; Kato, Nobuyuki; Shimotohno, Kunitada; Okazaki, Nobuo; Ohkoshi, Showgo; Uura, Masashi; Kaneko, Shuichi; Kobayashi, Kenichi; Omata, Masao; Ohnishi, Hiroo; Mutou, Yasutoshi; Hattori, Nobu.

In: Japanese Journal of Cancer Research, Vol. 82, No. 8, 08.1991, p. 879-882.

Research output: Contribution to journalArticle

Muraiso, K, Hijikata, M, Kato, N, Shimotohno, K, Okazaki, N, Ohkoshi, S, Uura, M, Kaneko, S, Kobayashi, K, Omata, M, Ohnishi, H, Mutou, Y & Hattori, N 1991, 'Detection of hepatitis C virus infection by enzyme-linked immunosorbent assay system using core protein expressed in Escherichia coli', Japanese Journal of Cancer Research, vol. 82, no. 8, pp. 879-882.
Muraiso, Kanae ; Hijikata, Makoto ; Kato, Nobuyuki ; Shimotohno, Kunitada ; Okazaki, Nobuo ; Ohkoshi, Showgo ; Uura, Masashi ; Kaneko, Shuichi ; Kobayashi, Kenichi ; Omata, Masao ; Ohnishi, Hiroo ; Mutou, Yasutoshi ; Hattori, Nobu. / Detection of hepatitis C virus infection by enzyme-linked immunosorbent assay system using core protein expressed in Escherichia coli. In: Japanese Journal of Cancer Research. 1991 ; Vol. 82, No. 8. pp. 879-882.
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