Detection of helicobacter hepaticus in human bile samples of patients with biliary disease

Toshihide Hamada, Kenji Yokota, Kiyoshi Ayada, Kazuyuki Hirai, Tomoari Kamada, Ken Haruma, Kazuaki Chayama, Keiji Oguma

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Background: Since the discovery of Helicobacter pylori, various enterohepatic Helicobacter spices have been detected in the guts of humans and animals. Some enterohepatic Helicobacters have been associated with inflammatory bowel disease or liver disease in mice. However the association of these bacteria with human diseases remains unknown. Materials and Methods: We collected 126 bile samples from patients with cholelithiasis, cholecystitis, gallbladder polyp, and other nonbiliary diseases. Samples were screened for the presence of enterohepatic Helicobacter spp. using cultures, nested PCR, or in situ hybridization. We tested for antibodies to H. pylori and H. hepaticus by Western blot analysis. Results: Attempts at cultivation were unsuccessful. However, H. hepaticus was detected in bile samples with nested PCR whereas H. bilis was not. Helicobacter hepaticus in the bile was confirmed by in situ hybridization, but H. hepaticus from bile samples was coccoid in appearance. We detected immunoglobulin G antibodies to H. hepaticus in bile samples by Western blotting. Helicobacter hepaticus was detected in 40 (32%) of total 126 samples as H. hepaticus positive if at least one of the three methods with nested PCR, in situ, or Western blotting. Patients with cholelithiasis (41%) and cholecystitis with gastric cancer (36%) had significantly higher (p =.029) prevalence of H. hepaticus infection than samples from patients with other diseases. Conclusion: Helicobacter hepaticus may closely associate with diseases of the liver and biliary tract in humans.

Original languageEnglish
Pages (from-to)545-551
Number of pages7
JournalHelicobacter
Volume14
Issue number6
DOIs
Publication statusPublished - Dec 2009

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Helicobacter hepaticus
Bile
Helicobacter
Cholecystitis
Cholelithiasis
Western Blotting
Helicobacter pylori
Polymerase Chain Reaction
In Situ Hybridization
Biliary Tract Diseases
Spices
Antibodies
Helicobacter Infections
Polyps
Gallbladder
Inflammatory Bowel Diseases
Stomach Neoplasms
Liver Diseases
Immunoglobulin G

Keywords

  • Bile
  • Cholecystitis
  • Gallbladder stone
  • Helicobacter hepaticus

ASJC Scopus subject areas

  • Gastroenterology
  • Infectious Diseases

Cite this

Detection of helicobacter hepaticus in human bile samples of patients with biliary disease. / Hamada, Toshihide; Yokota, Kenji; Ayada, Kiyoshi; Hirai, Kazuyuki; Kamada, Tomoari; Haruma, Ken; Chayama, Kazuaki; Oguma, Keiji.

In: Helicobacter, Vol. 14, No. 6, 12.2009, p. 545-551.

Research output: Contribution to journalArticle

Hamada, T, Yokota, K, Ayada, K, Hirai, K, Kamada, T, Haruma, K, Chayama, K & Oguma, K 2009, 'Detection of helicobacter hepaticus in human bile samples of patients with biliary disease', Helicobacter, vol. 14, no. 6, pp. 545-551. https://doi.org/10.1111/j.1523-5378.2009.00729.x
Hamada, Toshihide ; Yokota, Kenji ; Ayada, Kiyoshi ; Hirai, Kazuyuki ; Kamada, Tomoari ; Haruma, Ken ; Chayama, Kazuaki ; Oguma, Keiji. / Detection of helicobacter hepaticus in human bile samples of patients with biliary disease. In: Helicobacter. 2009 ; Vol. 14, No. 6. pp. 545-551.
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abstract = "Background: Since the discovery of Helicobacter pylori, various enterohepatic Helicobacter spices have been detected in the guts of humans and animals. Some enterohepatic Helicobacters have been associated with inflammatory bowel disease or liver disease in mice. However the association of these bacteria with human diseases remains unknown. Materials and Methods: We collected 126 bile samples from patients with cholelithiasis, cholecystitis, gallbladder polyp, and other nonbiliary diseases. Samples were screened for the presence of enterohepatic Helicobacter spp. using cultures, nested PCR, or in situ hybridization. We tested for antibodies to H. pylori and H. hepaticus by Western blot analysis. Results: Attempts at cultivation were unsuccessful. However, H. hepaticus was detected in bile samples with nested PCR whereas H. bilis was not. Helicobacter hepaticus in the bile was confirmed by in situ hybridization, but H. hepaticus from bile samples was coccoid in appearance. We detected immunoglobulin G antibodies to H. hepaticus in bile samples by Western blotting. Helicobacter hepaticus was detected in 40 (32{\%}) of total 126 samples as H. hepaticus positive if at least one of the three methods with nested PCR, in situ, or Western blotting. Patients with cholelithiasis (41{\%}) and cholecystitis with gastric cancer (36{\%}) had significantly higher (p =.029) prevalence of H. hepaticus infection than samples from patients with other diseases. Conclusion: Helicobacter hepaticus may closely associate with diseases of the liver and biliary tract in humans.",
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AU - Kamada, Tomoari

AU - Haruma, Ken

AU - Chayama, Kazuaki

AU - Oguma, Keiji

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