Abstract
We have successfully detected fibrillization process of amyloid-beta(1-40) protein (Aβ(1-40)) as causative compounds of Alzheimer's disease by our developed bio array sensing system utilizing different phospholipid liposomes encapsulating fluorescent molecules. The fluorescent intensity is dependent on the leakage of fluorescent molecules through perturbed lipid membrane of liposome. Therefore, the intensity considerably depends on the liposome-Aβ interaction strength on fibrillization process of Aβ(1-40). It is noted that the behavior of the fluorescent intensity corresponded to that of our cantilever sensor when measuring the interaction. Moreover, difference in Aβ(1-40) concentration or liposome-Aβ(1-40) interaction strength was successfully discriminated by principal component analysis. Consequently, we believe that the fluorescent liposome sensor is very effective for discriminating aggregation and fibrillization processes and concentrations of Aβ(1-40).
| Original language | English |
|---|---|
| Pages (from-to) | 1414-1417 |
| Number of pages | 4 |
| Journal | Procedia Engineering |
| Volume | 168 |
| DOIs | |
| Publication status | Published - 2016 |
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Keywords
- Amyloid Beta
- Array
- Biosensor
- Fluorescence
- Liposome
- Pricipal Component Analysis
ASJC Scopus subject areas
- Engineering(all)
Cite this
Detection of Fibrillization Process of Amyloid Beta Protein Using Arrayed Biosensor with Liposome Encapsulating Fluorescent Molecules. / Imamura, R.; Shimanouchi, Toshinori; Murata, N.; Yamashita, K.; Fukuzawa, M.; Noda, M.
In: Procedia Engineering, Vol. 168, 2016, p. 1414-1417.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of Fibrillization Process of Amyloid Beta Protein Using Arrayed Biosensor with Liposome Encapsulating Fluorescent Molecules
AU - Imamura, R.
AU - Shimanouchi, Toshinori
AU - Murata, N.
AU - Yamashita, K.
AU - Fukuzawa, M.
AU - Noda, M.
PY - 2016
Y1 - 2016
N2 - We have successfully detected fibrillization process of amyloid-beta(1-40) protein (Aβ(1-40)) as causative compounds of Alzheimer's disease by our developed bio array sensing system utilizing different phospholipid liposomes encapsulating fluorescent molecules. The fluorescent intensity is dependent on the leakage of fluorescent molecules through perturbed lipid membrane of liposome. Therefore, the intensity considerably depends on the liposome-Aβ interaction strength on fibrillization process of Aβ(1-40). It is noted that the behavior of the fluorescent intensity corresponded to that of our cantilever sensor when measuring the interaction. Moreover, difference in Aβ(1-40) concentration or liposome-Aβ(1-40) interaction strength was successfully discriminated by principal component analysis. Consequently, we believe that the fluorescent liposome sensor is very effective for discriminating aggregation and fibrillization processes and concentrations of Aβ(1-40).
AB - We have successfully detected fibrillization process of amyloid-beta(1-40) protein (Aβ(1-40)) as causative compounds of Alzheimer's disease by our developed bio array sensing system utilizing different phospholipid liposomes encapsulating fluorescent molecules. The fluorescent intensity is dependent on the leakage of fluorescent molecules through perturbed lipid membrane of liposome. Therefore, the intensity considerably depends on the liposome-Aβ interaction strength on fibrillization process of Aβ(1-40). It is noted that the behavior of the fluorescent intensity corresponded to that of our cantilever sensor when measuring the interaction. Moreover, difference in Aβ(1-40) concentration or liposome-Aβ(1-40) interaction strength was successfully discriminated by principal component analysis. Consequently, we believe that the fluorescent liposome sensor is very effective for discriminating aggregation and fibrillization processes and concentrations of Aβ(1-40).
KW - Amyloid Beta
KW - Array
KW - Biosensor
KW - Fluorescence
KW - Liposome
KW - Pricipal Component Analysis
UR - http://www.scopus.com/inward/record.url?scp=85009986102&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85009986102&partnerID=8YFLogxK
U2 - 10.1016/j.proeng.2016.11.400
DO - 10.1016/j.proeng.2016.11.400
M3 - Article
VL - 168
SP - 1414
EP - 1417
JO - Procedia Engineering
JF - Procedia Engineering
SN - 1877-7058
ER -