Detection of Fibrillization Process of Amyloid Beta Protein Using Arrayed Biosensor with Liposome Encapsulating Fluorescent Molecules

R. Imamura, Toshinori Shimanouchi, N. Murata, K. Yamashita, M. Fukuzawa, M. Noda

Research output: Contribution to journalArticle

Abstract

We have successfully detected fibrillization process of amyloid-beta(1-40) protein (Aβ(1-40)) as causative compounds of Alzheimer's disease by our developed bio array sensing system utilizing different phospholipid liposomes encapsulating fluorescent molecules. The fluorescent intensity is dependent on the leakage of fluorescent molecules through perturbed lipid membrane of liposome. Therefore, the intensity considerably depends on the liposome-Aβ interaction strength on fibrillization process of Aβ(1-40). It is noted that the behavior of the fluorescent intensity corresponded to that of our cantilever sensor when measuring the interaction. Moreover, difference in Aβ(1-40) concentration or liposome-Aβ(1-40) interaction strength was successfully discriminated by principal component analysis. Consequently, we believe that the fluorescent liposome sensor is very effective for discriminating aggregation and fibrillization processes and concentrations of Aβ(1-40).

Original languageEnglish
Pages (from-to)1414-1417
Number of pages4
JournalProcedia Engineering
Volume168
DOIs
Publication statusPublished - 2016

Keywords

  • Amyloid Beta
  • Array
  • Biosensor
  • Fluorescence
  • Liposome
  • Pricipal Component Analysis

ASJC Scopus subject areas

  • Engineering(all)

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