Abstract
We have successfully detected fibrillization process of amyloid-beta(1-40) protein (Aβ(1-40)) as causative compounds of Alzheimer's disease by our developed bio array sensing system utilizing different phospholipid liposomes encapsulating fluorescent molecules. The fluorescent intensity is dependent on the leakage of fluorescent molecules through perturbed lipid membrane of liposome. Therefore, the intensity considerably depends on the liposome-Aβ interaction strength on fibrillization process of Aβ(1-40). It is noted that the behavior of the fluorescent intensity corresponded to that of our cantilever sensor when measuring the interaction. Moreover, difference in Aβ(1-40) concentration or liposome-Aβ(1-40) interaction strength was successfully discriminated by principal component analysis. Consequently, we believe that the fluorescent liposome sensor is very effective for discriminating aggregation and fibrillization processes and concentrations of Aβ(1-40).
Original language | English |
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Pages (from-to) | 1414-1417 |
Number of pages | 4 |
Journal | Procedia Engineering |
Volume | 168 |
DOIs | |
Publication status | Published - 2016 |
Event | 30th Eurosensors Conference, Eurosensors 2016 - Budapest, Hungary Duration: Sep 4 2016 → Sep 7 2016 |
Keywords
- Amyloid Beta
- Array
- Biosensor
- Fluorescence
- Liposome
- Pricipal Component Analysis
ASJC Scopus subject areas
- Engineering(all)