Detection of Epstein-Barr Virus RNA and Related Antigens in Non-Neoplastic Lymphoid Lesions

Yuji Tonoyama, Norihiro Teramoto, Ashit Baran Sarker, Tadashi Yoshino, Kazuhiko Hayashi, Kiyoshi Takahashi, Tadaatsu Akagi

Research output: Contribution to journalArticle

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Abstract

To elucidate the latent state and reactivation of Epstein-Barr virus (EBV) in non-neoplastic lymphoid lesions, we investigated 144 non-neoplastic lymphoid lesions by in situ hybridization (ISH) to detect the expression of EBV-encoded small RNAs (EBER)-1 and BCRF-1 and by immunostaining for latent membrane protein (LMP)-1 and ZEBRA. ISH for EBER-1 detected EBER-1-positive cells (EPC) in 31 of the 144 examined lesions (22%). EPC were detected in 4 of 49 cases of nonspecific lymphoid hyperplasia, in 16 of 20 abscess-forming granulomatous lymphadenitis (AFGL), 5 of 25 Kikuchi's disease, and in 3 of 3 infectious mononucleosis. LMP-1 was expressed in 6 of 124 non-neoplastic lymphoid lesions (4.8%). LMP-1-positive cells were observed in 6 of the 31 EBER-1-positive cases (19%). EPC were detected significantly more frequently in LMP-1- and ZEBRA-positive specimens than in the LMP-1- and ZEBRA-negative specimens. BCRF-1 was expressed in 4 of 11 cases examined: 2 of 3 AFGL, 1 of 2 Kikuchi's disease, and in the 1 case of atypical lymphoid hyperplasia. This study suggests that Epstein-Barr virus is prevalent and can be reactivated in the lymph nodes effaced by destructive inflammation, such as AFGL. Such inflammation may provide a local milieu that is conducive for EBV to enter the lytic cycle.

Original languageEnglish
Pages (from-to)89-96
Number of pages8
JournalActa Medica Okayama
Volume50
Issue number2
Publication statusPublished - Apr 1996

Fingerprint

Human Herpesvirus 4
Viruses
Membrane Proteins
RNA
Antigens
Lymphadenitis
Histiocytic Necrotizing Lymphadenitis
Abscess
Hyperplasia
In Situ Hybridization
Cells
Inflammation
Infectious Mononucleosis
Epstein-Barr virus encoded RNA 1
Lymph Nodes

Keywords

  • BCRF-I
  • EBER-I
  • LMP-I
  • Lymphoid lesion
  • ZEBRA

ASJC Scopus subject areas

  • Surgery
  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tonoyama, Y., Teramoto, N., Sarker, A. B., Yoshino, T., Hayashi, K., Takahashi, K., & Akagi, T. (1996). Detection of Epstein-Barr Virus RNA and Related Antigens in Non-Neoplastic Lymphoid Lesions. Acta Medica Okayama, 50(2), 89-96.

Detection of Epstein-Barr Virus RNA and Related Antigens in Non-Neoplastic Lymphoid Lesions. / Tonoyama, Yuji; Teramoto, Norihiro; Sarker, Ashit Baran; Yoshino, Tadashi; Hayashi, Kazuhiko; Takahashi, Kiyoshi; Akagi, Tadaatsu.

In: Acta Medica Okayama, Vol. 50, No. 2, 04.1996, p. 89-96.

Research output: Contribution to journalArticle

Tonoyama, Y, Teramoto, N, Sarker, AB, Yoshino, T, Hayashi, K, Takahashi, K & Akagi, T 1996, 'Detection of Epstein-Barr Virus RNA and Related Antigens in Non-Neoplastic Lymphoid Lesions', Acta Medica Okayama, vol. 50, no. 2, pp. 89-96.
Tonoyama Y, Teramoto N, Sarker AB, Yoshino T, Hayashi K, Takahashi K et al. Detection of Epstein-Barr Virus RNA and Related Antigens in Non-Neoplastic Lymphoid Lesions. Acta Medica Okayama. 1996 Apr;50(2):89-96.
Tonoyama, Yuji ; Teramoto, Norihiro ; Sarker, Ashit Baran ; Yoshino, Tadashi ; Hayashi, Kazuhiko ; Takahashi, Kiyoshi ; Akagi, Tadaatsu. / Detection of Epstein-Barr Virus RNA and Related Antigens in Non-Neoplastic Lymphoid Lesions. In: Acta Medica Okayama. 1996 ; Vol. 50, No. 2. pp. 89-96.
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