Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies specific for the native conformation

Norihisa Yasui, Emiko Mihara, Maiko Nampo, Keiko Tamura-Kawakami, Hideaki Unno, Kyoichi Matsumoto, Junichi Takagi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

LRP6 is a cell surface molecule that plays a critical role in the Wnt signaling pathway, and is implicated in numerous human diseases. Studies of cellular signaling mediated by LRP6 have relied on overexpression experiments, due to the lack of good monoclonal antibodies (mAbs) reactive with native LRP6 ectodomain. By using native recombinant LRP6 ectodomain fragment produced in mammalian expression system, we succeeded in developing a panel of anti-human LRP6 mAbs. Selected mAbs were capable of staining endogenous LRP6 on cell surface by using flow cytometry and immunofluorescence microscopy, and enriching detergent-solubilized LRP6 from cell lysate by immunoprecipitation.

Original languageEnglish
Pages (from-to)153-160
Number of pages8
JournalJournal of Immunological Methods
Volume352
Issue number1-2
DOIs
Publication statusPublished - Jan 31 2010
Externally publishedYes

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Monoclonal Antibodies
Wnt Signaling Pathway
Fluorescence Microscopy
Immunoprecipitation
Detergents
Flow Cytometry
Staining and Labeling

Keywords

  • LRP6
  • Monoclonal antibody
  • Wnt

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies specific for the native conformation. / Yasui, Norihisa; Mihara, Emiko; Nampo, Maiko; Tamura-Kawakami, Keiko; Unno, Hideaki; Matsumoto, Kyoichi; Takagi, Junichi.

In: Journal of Immunological Methods, Vol. 352, No. 1-2, 31.01.2010, p. 153-160.

Research output: Contribution to journalArticle

Yasui, Norihisa ; Mihara, Emiko ; Nampo, Maiko ; Tamura-Kawakami, Keiko ; Unno, Hideaki ; Matsumoto, Kyoichi ; Takagi, Junichi. / Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies specific for the native conformation. In: Journal of Immunological Methods. 2010 ; Vol. 352, No. 1-2. pp. 153-160.
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