Detection of codon 61 point mutations of the K-ras gene in lung and colorectal cancers by enriched PCR

Shinichi Toyooka, Kazunori Tsukuda, Mamoru Ouchida, Motohiko Tanino, Yasuhiko Inaki, Kazuyasu Kobayashi, Masaaki Yano, Junichi Soh, Takuya Kobatake, Nobuyoshi Shimizu, Kenji Shimizu

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45 Citations (Scopus)

Abstract

Mutation of the Kirsten ras (K-ras) gene is one of most common alterations in solid tumors including lung and colorectal cancers. We developed new enriched PCR-RFLP assay to detect mutations of K-ras codon 61 at the 1st and 2nd letters and non-enriched PCR-RFLP assay to detect the 3rd letter mutation. One mutant allele among 103 wild-type alleles was detected by enriched PCR-RFLP assay, while one mutant in 10 wild-type alleles was detected by non-enriched PCR-RFLP assay for codon 61 3rd letter. We then examined K-ras codon 12, 13 and 61 mutations in lung and colorectal cancers using these assays. K-ras codon 12 mutation was detected in 10 of 109 (9%) lung cancer and 19 of 83 (23%) colorectal cancer cases. K-ras codon 13 mutation was detected in 2 of 83 (2%) colorectal and 0 of 109 NSCLC cases, respectively. There was no K-ras codon 61 mutation in either type of cancer. Our results demonstrate that enriched PCR-RFLP is a sensitive assay to detect K-ras codon 61 mutation, however, it was extremely rare in lung and colorectal cancers, suggesting organ-specific pathways in mutagenesis of the ras gene family.

Original languageEnglish
Pages (from-to)1455-1459
Number of pages5
JournalOncology reports
Volume10
Issue number5
DOIs
Publication statusPublished - Sep 2003

Keywords

  • Colorectal cancer
  • Enriched PCR
  • Kirsten ras
  • Lung cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Toyooka, S., Tsukuda, K., Ouchida, M., Tanino, M., Inaki, Y., Kobayashi, K., Yano, M., Soh, J., Kobatake, T., Shimizu, N., & Shimizu, K. (2003). Detection of codon 61 point mutations of the K-ras gene in lung and colorectal cancers by enriched PCR. Oncology reports, 10(5), 1455-1459. https://doi.org/10.3892/or.10.5.1455